Flavobacterium aquatile and Flavobacterium meningosepticum: Glucose Nonfermenters with Similar Flagellar Morphologies

Webster, John; Hugh, Rudolph

doi:10.1099/00207713-29-4-333

Cited by 8 publications

(4 citation statements)

References 23 publications

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“…These pseudoflagella were seen on cells after silver impregnation and on chromium-shadowed cells under the electron microscope, but they were not seen on cells stained by Leifson's procedure. In the case of F. meningosepticum, the results of Webster and Hugh (27) represent the first reported observation of motility in this species, and we believe further confirmation by electron microscopy of the nature of the structures observed is required.…”

Section: Discussionmentioning

confidence: 63%

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Flavobacterium multivorum, a New Species Isolated from Human Clinical Specimens and Previously Known as Group IIk, Biotype 2

Holmes¹,

Owen²,

Weaver

1981

International Journal of Systematic Bacteriology

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Thirty-two gram-negative bacterial strains, most of which were yellow-pig-mented and isolated from clinical sources, were compared with 17 culture collection strains representing 13 named strains of four Flavobacterium species and 4 unnamed strains of Flavobacterium species. Twenty-nine of the strains were originally received as or subsequently assigned to group IIk, biotype 2. All 49 strains were examined for 127 characteristics, including reactions to 58 enzyme tests (API ZYM system), and the data were analyzed by numerical taxonomic methods involving estimation of Gower's general similarity coefficient and clustering by unweighted pair-group average linkage and single-linkage analyses. Twenty-eight of the group IIk, biotype 2, strains formed a single, well-defined cluster (92.0% similarity level). These 28 strains were aerobic, gram-negative, nonmotile, oxidative rods, the mean base composition of the deoxyribonucleic acids of 11 strains was 39.6 f 0.5 mol% guanine plus cytosine. Distinctive features of the 28 strains were the ability to produce acid from a range of carbohydrates and their resistance to antimicrobial agents. The 28 strains were classified in the genus Flauobacterium, but they could not be assigned to any previously defined species. Therefore a new species, with the name Flavobacterium multivorum, is proposed for these 28 strains; the type strain is B5533 (= NCTC 11343). Tatum et al. (25) described 140 strains of gram-negative, yellow-pigmented, rod-shaped bacteria thought to resemble Pseudomonas or possibly Xanthomonas species isolated from human and other sources of clinical interest. These bacteria were referred to as "group IIk," and two biotypes were recognized (although the word "biovar" is now recommended in place of "bio-type" [16], we continue to use "biotype" here because of its historical association with group IIk). The 100 strains of biotype 1 were deeply yellow pigmented, whereas the 40 strains of bio-type 2 were usually pale to light yellow. The motility observations of the group IIk strains in semisolid agar medium were sometimes equivocal , hence, wet-mount preparations were recommended. Five percent of the biotype 2 strains were reported as motile, and for both biotypes of group IIk, strains were described as monotri-chous (25). Holmes et al. (10) studied various yellow-pig-mented isolates originally received at the National Collection of Type Cultures (NCTC), London, United Kingdom, for identification and showed, by numerical analysis of phenotypic characteristics, that 27 strains formed a cluster with two reference strains of group IIk, biotype 1. The name Pseudomonas paucimobilis was proposed by Holmes et al. (10) for these 29 strains because most motile strains contained few actively motile cells. Motile strains had a single polar flagellum. Holmes et al. excluded two reference strains of group IIk, biotype 2, and two biochemically similar strains from the P. paucimo bilis cluster. Deoxyribonucleic acid (DNA) base compositions (moles percent gua-nine plus cytosine [G+C] p...

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Section: Discussionmentioning

confidence: 63%

“…1979, C(H)32, p. 3511, were nonmotile. The presence of flagella has been reported on cells of the type strain of Flavobacterium aquatile and of the type strain of F. meningosepticurn (27). Weeks (28) described structures on F. aquatile strain F36 that did not appear to be true flagella and which he considered similar to pseudoflagella.…”

Section: Discussionmentioning

confidence: 99%

Flavobacterium multivorum, a New Species Isolated from Human Clinical Specimens and Previously Known as Group IIk, Biotype 2

Holmes¹,

Owen²,

Weaver

1981

International Journal of Systematic Bacteriology

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“…burtonensis ACAM 188 T [14], F. aquatile LMG 4008 T [15] and W. aurantia WNB302 T [16] were used as the reference strains to compare with strain CWB-1 T .…”

Section: Phylogeny Based On the 16s Rrna And Rpob Gene Sequencesmentioning

confidence: 99%

Paucihalobacter ruber gen. nov., sp. nov., isolated from a haloalkaline lake sediment sample

Wang

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped bacterium, designated CWB-1T, was isolated from a haloalkaline lake sediment sample collected from the bottom of Chaiwopu Lake, Urumchi, Xinjiang Province, PR China. Strain CWB-1T grew at 4–40 °C (optimum, 30–35 °C), pH 6.5–9.0 (optimum, pH 6.5–7.0) and with 0.5–5.5 % (w/v) NaCl (optimum, 2.5–3.0 %). Phylogenetic analyses based on the 16S rRNA gene sequence and the whole genome sequence both revealed that strain CWB-1T belonged to the family Flavobacteriaceae . The strain had the highest similarity of the 16S rRNA gene sequence to Psychroserpens jangbogonensis PAMC 27130T (92.8 %). The genome of strain CWB-1T was 3 548 011 bp long with 36.3 % DNA G+C content. The predominant fatty acids (>10 %) in the CWB-1T cells were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 1 (iso-C15 : 1 H/C13 : 0 3-OH). The major respiratory quinone was menaquinone-6 and the major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and two unidentified lipids. Based on the phylogenetic analyses, as well as the phenotypic characteristics, a novel genus and species of the family Flavobacteriaceae , Paucihalobacter ruber gen. nov., sp. nov., is proposed. The type strain is CWB-1T (=KCTC 72450T=CGMCC 1.17149T).

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“…In addition, other microbial populations that metabolized on the exudates from microalgae co-exist in the photo-bioreactors. For example, certain species within Flavobacterium are fermentative bacteria and can utilize the metabolites from microalgae as carbon sources [41][42][43]. This observation reiterates that in large-scale applications of microalgal systems, it may be difficult to implement pure cultures for photo-bioreactor operation, and that maintaining a mixed culture may be more effective for further treatment of wastewaters [44,45].…”

Section: Differences In 16s Rrna Gene-based Communities Among Reactorsmentioning

confidence: 99%

Increasing tetracycline concentrations on the performance and communities of mixed microalgae-bacteria photo-bioreactors

et al. 2018

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This study investigated the impact of varying concentrations of tetracycline on the performance of mixed microalgae-bacteria photo-bioreactors. Photo-bioreactors were assessed for their ability to remove carbon dioxide (CO2) from the biogas of anaerobic membrane bioreactor (anMBR), and nutrients from the anaerobic effluent. The varying concentrations of tetracycline had no impact on the removal of CO2 from biogas. 29% v/v of CO2 was completely removed to generate > 20% v/v of oxygen (O2) in all reactors. Removal of nutrients and biomass was not affected at low concentrations of tetracycline (<=150 µg/L), but 20 mg/L of tetracycline lowered the biomass generation and removal efficiencies of phosphate. Conversely, high chlorophyll a and b content was observed at 20 mg/L of tetracycline. High tetracycline level had no impact on the diversity of 18S rRNA gene-based microalgal communities but adversely affected the 16S rRNA gene-based microbial communities. Specifically, both Proteobacteria and Bacteroidetes phyla decreased in relative abundance but not phylum Chloroplast. Additionally, both nitrogenfixing (e.g. Flavobacterium, unclassified Burkholderiales and unclassified Rhizobiaceae) and denitrifying groups (e.g. Hydrogenophaga spp.) were significantly reduced in relative abundance at high tetracycline concentration. Phosphate-accumulating microorganisms, Acinetobacter spp. and Pseudomonas spp. were similarly reduced upon exposure to high tetracycline concentration. Unclassified Comamonadaceae, however, increased in relative abundance, which correlated with an increase in the abundance of tetracycline resistance genes associated with efflux pump mechanism. Overall, the findings demonstrate that antibiotic concentrations in municipal wastewaters will not significantly affect the removal of nutrients by the mixed microalgaebacteria photo-bioreactors. However, utilizing such photo-bioreactors as a polishing step for anMBRs that treat wastewaters with high tetracycline concentration may not be effective as evidenced from the lower nutrient removal and occurrence of antibiotic resistance genes. Highlights • Microalgal biomass yield and removal of CO2 from biogas unaffected by tetracycline • Removal of nutrient from anaerobic effluent affected by 20 mg/L of tetracycline • Microalgae community insignificantly affected by 20 mg/L of tetracycline • Bacterial populations, including those involved in N and P cycling, affected by 20 mg/L tetracycline

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Flavobacterium aquatile and Flavobacterium meningosepticum: Glucose Nonfermenters with Similar Flagellar Morphologies

Cited by 8 publications

References 23 publications

Flavobacterium multivorum, a New Species Isolated from Human Clinical Specimens and Previously Known as Group IIk, Biotype 2

Flavobacterium multivorum, a New Species Isolated from Human Clinical Specimens and Previously Known as Group IIk, Biotype 2

Paucihalobacter ruber gen. nov., sp. nov., isolated from a haloalkaline lake sediment sample

Increasing tetracycline concentrations on the performance and communities of mixed microalgae-bacteria photo-bioreactors

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