Flagellum Abnormalities of Spermatozoa in Seminiferous Tubules after a Short Term Vasectomy.

Hamasaki, Masao; Miyajima, Satoshi; Murakami, Masahirô

doi:10.1679/aohc.54.391

Cited by 4 publications

(1 citation statement)

References 20 publications

(16 reference statements)

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“…To date, there is no unanimity in opinion among authors. While some did not find alterations in rat (Flickinger, 1972; McDonald and Scothorne, 1988), rabbit (Paufler and Foote, 1969; Flickinger, 1975a,b), and monkey (Chapman et al, 1978; Hadley and Dym, 1983), others reported marked changes in mouse (Singh and Chakravarty, 2000), rat (Flickinger et al, 1986, 1987, 1990; McDonald et al, 1996; Sarrat et al, 1996; Aydos et al, 1998; Whyte et al, 2000), hamster (Hamasaki et al, 1991; Sun et al, 1992), guinea pig (Hutson et al, 1976; Tung and Alexander, 1977), rabbit (Swanson and Hafs, 1969; Hooker, 1980), dog (Antypas et al, 1994), and monkey (Lohiya et al, 1987).…”

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confidence: 99%

Effect of Vasectomy on Sperm Nuclear Chromatin Condensation in the Rabbit

Steger

Slavov

Failing³

et al. 2005

Journal of Andrology

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Histone-to-protamine exchange in haploid spermatids is known to play a central role for male fertility. The present study investigates, for the first time, the effects of vasectomy on the expression of protamines in the rabbit. During normal spermatogenesis, protamine-1 and protamine-2 mRNA were expressed from step 5 round spermatids to step 11 elongated spermatids. In unilaterally vasectomized animals, control testes revealed normal spermatogenesis with normal protamine expression, while vasectomized testes exhibited both normal spermatogenesis and spermatogenic arrest. Some testes with normal spermatogenesis revealed delayed expression of both protamine-1 and protamine-2. Furthermore, multinucleated round spermatids were a regular finding in these testes. In both treated and untreated animals, a higher percentage of spermatozoa from the cauda epididymis had highly condensed chromatin when compared with those from the testis. The percentage of spermatozoa with highly condensed chromatin from testes and epididymides from the vasectomized side of treated animals remained unchanged from controls. As the integrity of nuclear chromatin is important for oocyte fertilization, especially in intracytoplasmic sperm injection (ICSI), where most of the natural selection mechanisms are bypassed, our data add valuable information for the treatment of infertility by ICSI, showing that vasectomy may affect nuclear chromatin integrity of testicular spermatids but not epididymal spermatozoa. Microsurgical epididymal sperm aspiration (MESA), therefore, may be superior to testicular sperm extraction (TESE) in vasectomized patients.

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confidence: 99%

Effect of Vasectomy on Sperm Nuclear Chromatin Condensation in the Rabbit

Steger

Slavov

Failing³

et al. 2005

Journal of Andrology

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Early Effects of Vasectomy on Testicular Structure and on Germ Cell and Macrophage Apoptosis in the Hamster

LUE,

HIKIM,

WANG

et al. 1997

Journal of Andrology

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This study provides quantitative information on the early (up to 3 months) effects of vasectomy on apoptosis in the hamster testis. Groups of five adult male golden hamsters were either bilaterally vasectomized or sham‐operated and sacrificed at intervals of 3, 6, and 12 weeks after surgery. In all three postvasectomy groups, testis weight and testicular and plasma testosterone (T) levels were not different from controls. Spermatogenic alterations, ranging from tubules with mild intraepithelial vacuoles to almost completely atrophied tubules, were detected in samples of 1 of 5 testes both at 3 and 12 weeks after vasectomy. Histometric analysis of testicular tissues at 3, 6, and 12 weeks in the postvasectomy groups showed no discernible effect of vasectomy on the absolute volumes of seminiferous tubules, tubular lumen, and total Leydig cells when compared to respective controls. In situ analysis of germ‐cell apoptosis, characterized by 3′ ‐end‐labeling immunocytochemistry, revealed a significant increase (2.5‐fold) in germ‐cell apoptosis at stages XIII‐I, involving primarily the dividing spermatocytes after 3 weeks of vasectomy. Apoptotic index was not changed from sham‐operated animals at 6 and 12 weeks postvasectomy. Interestingly, a very high incidence of macrophage apoptosis was detected in the samples of three out of five testes in the 12 weeks postvasectomy group (39.3%) compared to that of controls (0.8%). These results demonstrate that vasectomy has little or no detrimental effect on the morphologic characteristics of the spermatogenesis or intratesticular concentrations of testosterone in the majority of the animals studied up to 12 weeks postsurgery, although vasectomy transiently (3 weeks postsurgery) activated germ‐cell apoptosis, involving dividing spermatocytes at stages XIII‐I.

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Cellular responses to vasectomy

McDonald

2000

International Review of Cytology

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Flagellum Abnormalities of Spermatozoa in Seminiferous Tubules after a Short Term Vasectomy.

Cited by 4 publications

References 20 publications

Effect of Vasectomy on Sperm Nuclear Chromatin Condensation in the Rabbit

Effect of Vasectomy on Sperm Nuclear Chromatin Condensation in the Rabbit

Early Effects of Vasectomy on Testicular Structure and on Germ Cell and Macrophage Apoptosis in the Hamster

Cellular responses to vasectomy

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