Five steroidal components from the rhizomes of Polygonatum odoratum var. pluriflorum.

Sugiyama, Mari; Nakano, Kenji; Tomimatsu, Toshiaki; Nohara, Toshihiro

doi:10.1248/cpb.32.1365

Cited by 40 publications

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“…Many active compounds have been identified in P. odoratum such as mucous polysaccharides [ 6 ], homoisoflavanones [ 7 ], azetidine-2-carboxylic acid [ 5 , 8 ], saponin [ 9 ], and steroidal compounds [ 10 ]. Among them, polysaccharides are abundant in P. odoratum and considered as one of the important bioactive components with functions of antitumor, antidiabetes, and antioxidation.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Alkaline Extraction and Bioactivities of Polysaccharides from Rhizome ofPolygonatum odoratum

Chen

Yin

Zhang

et al. 2014

BioMed Research International

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The present study is to explore the optimal extraction parameters, antioxidant activity, and antimicrobial activity of alkaline soluble polysaccharides from rhizome of Polygonatum odoratum. The optimal extraction parameters were determined as the following: NaOH concentration (A) 0.3 M, temperature (B) 80°C, ratio of NaOH to solid (C) 10-fold, and extraction time (D) 4 h, in which ratio of NaOH to solid was a key factor. The order of the factors was ratio of NaOH to solid (fold, C) > extraction temperature (°C, B) > NaOH concentration (M, A) > extraction time (h, D). The monosaccharide compositions of polysaccharides from P. odoratum were rhamnose, mannose, xylose, and arabinose with the molecular ratio of 31.78, 31.89, 11.11, and 1.00, respectively. The reducing power, the 1, 1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging rate, the hydroxyl radicals scavenging rate, and the inhibition rate to polyunsaturated fatty acid (PUFA) peroxidation of the alkaline soluble polysaccharides from P. odoratum at 1 mg/mL were 9.81%, 52.84%, 19.22%, and 19.42% of ascorbic acid at the same concentration, respectively. They also showed antimicrobial activity against pathogenic bacteria Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, and Escherichia coli.

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Section: Introductionmentioning

confidence: 99%

Optimization of Alkaline Extraction and Bioactivities of Polysaccharides from Rhizome ofPolygonatum odoratum

Chen

Yin

Zhang

et al. 2014

BioMed Research International

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“…The MeOH-eluted and MeOH-H 2 O (6:4)-eluted fractions were then subjected to silica gel and octadecylsilanized (ODS) silica gel column chromatography (CC) and reversed-phase preparative high-performance liquid chromatography (HPLC) to obtain compounds 1 – 15 ( Figure 1 ). The structures of the known compounds 1 – 3 and 7 – 9 were identified as (25 S )-spirost-5-en-3β-yl O -β- d -glucopyranosyl-(1→2)- O -[β- d -xylopyranosyl-(1→3)]- O -β- d -glucopyranosyl-(1→4)-β- d -galactopyranoside ( 1 ) [ 5 ], (25 S )-14α-hydroxyspirost-5-en-3β-yl O-β- d -glucopyranosyl-(1→2)- O -[β- d -xylopyranosyl-(1→3)]- O -β- d -glucopyranosyl-(1→4)-β- d -galactopyranoside ( 2 ) [ 5 ], (25 R )-spirost-5-en-3β-yl O -α-L-rhamnopyranosyl-(1→4)-β- d -glucopyranoside ( 3 ) [ 6 ], (25 S )-26-[(β- d -glucopyranosyl)oxy]-22α-hydroxyfurost-5-en-3β-ol ( 7 ) [ 7 ], (25 S )-26-[(β- d -glucopyranosyl)oxy]-22α-hydroxyfurost-5-en-3β-yl O -β- d -glucopyranosyl-(1→2)- O -[β- d -xylopyranosyl-(1→3)]- O -β- d -glucopyranosyl-(1→4)-β- d -galactopyranoside ( 8 ) [ 8 ], and (25 S )-26-[(β- d -glucopyranosyl)oxy]-14α,22α-hydroxyfurost-5-en-3β-yl O -β- d -glucopyranosyl-(1→2)- O -[β- d -xylopyranosyl-(1→3)]- O -β- d -glucopyranosyl-(1→4)-β- d -galactopyranoside ( 9 ) [ 9 ], respectively.…”

Section: Resultsmentioning

confidence: 99%

Steroidal Glycosides from Convallaria majalis Whole Plants and Their Cytotoxic Activity

Matsuo

Shinoda

Nakamaru

et al. 2017

IJMS

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Phytochemical examination of Convallaria majalis (Liliaceae) whole plants yielded 15 steroidal glycosides (1–15), including nine new compounds (4–6, 10–15) with a lycotetrose unit. The structures of the new compounds were determined using two-dimensional Nuclear magnetic resonance (NMR) analyses and chemical methods. The isolated compounds were evaluated for cytotoxicity against HL-60 human promyelocytic leukemia cells, A549 human lung adenocarcinoma cells, and HSC-4 and HSC-2 human oral squamous cell carcinoma cell lines. Of these, (25S)-spirost-5-en-3β-yl O-β-d-glucopyranosyl-(1→2)-O-[β-d-xylopyranosyl-(1→3)]-O-β-d-glucopyranosyl-(1→4)-β-d-galactopyranoside (1) exhibited cytotoxic activity against HL-60, A549, HSC-4, and HSC-2 cells with IC50 values ranging from 0.96 to 3.15 μM. The corresponding furostanol glycoside of 1, (25S)-26-[(β-d-glucopyranosyl)oxy]-22α-hydroxyfurost-5-en-3β-yl O-β-d-glucopyranosyl-(1→2)-O-[β-d-xylopyranosyl-(1→3)]-O-β-d-glucopyranosyl-(1→4)-β-d-galactopyranoside (8), was cytotoxic to the adherent cell lines of A549, HSC-4, and HSC-2 cells with IC50 values of 2.97, 11.04, and 8.25 μM, respectively. The spirostanol lycotetroside (1) caused necrotic cell death in A549 cells in a dose-dependent manner. Alternatively, the furostanol lycotetroside (8) induced apoptotic cell death in A549 cells in a time-dependent manner, as was evident by morphological observations and flow cytometry analyses.

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“…Seventy-eight steroidal saponins had been isolated from twelve Polygonatum species up to 2006 [18][19][20][21][22][23][24][25][26][27], of which 42 were novel compounds [28].…”

Section: Steroidal Saponinsmentioning

confidence: 99%

Chemical Constituents of the Genus Polygonatum and their Role in Medicinal Treatment

Zhao

2015

Natural Product Communications

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Polygonatum is a famous traditional Chinese medicine that is widely used in China, Korea and Japan. In the last decade, constituents of the genus have been reported including steroidal saponins, flavones, alkaloids, lignins, amino acids and carbohydrates, some of which show biological properties such as antiviral and antitumor activity, variable effects on the immune system and anticoagulant activity. In addition, some findings provide novel evidence that Polygonatum species may contain potential anti-tumor and anti-viral proteins for possible medical application and large-scale pharmaceutical production. In this review, we focus on the updated research of the chemical constituents of Polygonatum including polysaccharides, steroidal saponins, flavonoids and lectins, and their potential therapeutic roles.

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Five steroidal components from the rhizomes of Polygonatum odoratum var. pluriflorum.

Cited by 40 publications

References 0 publications

Optimization of Alkaline Extraction and Bioactivities of Polysaccharides from Rhizome ofPolygonatum odoratum

Optimization of Alkaline Extraction and Bioactivities of Polysaccharides from Rhizome ofPolygonatum odoratum

Steroidal Glycosides from Convallaria majalis Whole Plants and Their Cytotoxic Activity

Chemical Constituents of the Genus Polygonatum and their Role in Medicinal Treatment

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