Fission yeast cut5 links nuclear chromatin and M phase regulator in the replication checkpoint control.

Saka, Yasushi; Fantes, Peter A.; Sutani, Takashi; McInerny, Christopher; Creanor, J.; Yanagida, Mitsuhiro

doi:10.1002/j.1460-2075.1994.tb06866.x

Cited by 135 publications

(121 citation statements)

References 60 publications

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“…The double mutants between mis3-224 (Takahashi et al e1994) and various mutations noted below were constructed; cdc2-33, cdc6-121, cdc13-117, cdc25-22, cdc10-129 (Nurse et al 1976), cdc2-3w (Enoch et al 1992), Dcyc17/cig2 (Obara-Ishihara & Okayama 1994), Dmik1 (Lundgren et al 1991), wee1-50 (Russell & Nurse 1987), cdc16-116 (Fankhauser et al 1993), cdc17-K42, cdc18-K46 (Nasmyth & Nurse 1981), cdc22-C1 (Fernandez Sarabia et al 1993), swi7-H4 (Singh & Klar 1993), mis5-268, mis11-453 (Takahashi et al 1994, Drad3, Drad9, Drad17, Drad24, Drad25, Dchk1 (Al-Khodairy et al 1994), cut5-T401 (Saka et al 1994), Dcds1 (Murakami & Okayama 1995), Ddis1 (Nabeshima et al 1995), dis3-54 (Kinoshita et al 1991), dis2-11, Ddis2 (Ohkura et al 1989), cut1-21, cut2-364 (Funabiki et al 1996, cut9 (Yamada et al 1997), Dppa1, Dppa2 (Kinoshita et al 1993),Dpck1 (Toda et al 1993), Dtop1, top2-191 (Uemura & Yanagida 1984), and Ddsk1 mutants (Takeuchi & Yanagida 1993). The Mis3-HA integrant was obtained by integrating the HA-tagged mis3 gene at the carboxyl-terminus to replace the genomic mis3 locus.…”

Section: Strains Genetical Methods and Macromolecular Analysismentioning

confidence: 99%

Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint

2000

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Section: Strains Genetical Methods and Macromolecular Analysismentioning

confidence: 99%

Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint

2000

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“…A number of gene products essential for the initiation of DNA replication (such as Cdc18, Cdt1, Orp1, Pol␣, and Rad4/Cut5) are known to be required also for induction of replication checkpoint signals, because loss or inactivation of these proteins causes an aberrant mitosis without DNA replication (Kelly et al, 1993;Saka and Yanagida, 1993;Hofmann and Beach, 1994;Saka at al., 1994;Grallert and Nurse, 1996;Bhaumik and Wang, 1998). Formation of cut cells in hsk1-89 at 30°C, which was also seen in hsk1 null mutants albeit with lesser frequency (Masai et al, 1995), is likely to be caused by defect in the same checkpoint control generated by the block in initiation of DNA synthesis.…”

Section: Replication Checkpoint Signaling Is Defective In Hsk1-89 Mutantmentioning

confidence: 99%

Regulation of Initiation of S Phase, Replication Checkpoint Signaling, and Maintenance of Mitotic Chromosome Structures during S Phase by Hsk1 Kinase in the Fission Yeast

Takeda

Ogino

Tatebayashi

et al. 2001

MBoC

105

132

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Hsk1, Saccharomyces cerevisiae Cdc7-related kinase in Shizosaccharomyces pombe, is required for G1/S transition and its kinase activity is controlled by the regulatory subunit Dfp1/Him1. Analyses of a newly isolated temperature-sensitive mutant, hsk1-89, reveal that Hsk1 plays crucial roles in DNA replication checkpoint signaling and maintenance of proper chromatin structures during mitotic S phase through regulating the functions of Rad3 (ATM)-Cds1 and Rad21 (cohesin), respectively, in addition to expected essential roles for initiation of mitotic DNA replication through phosphorylating Cdc19 (Mcm2). Checkpoint defect in hsk1-89 is indicated by accumulation of cut cells at 30°C. hsk1-89 displays synthetic lethality in combination with rad3 deletion, indicating that survival of hsk1-89 depends on Rad3-dependent checkpoint pathway. Cds1 kinase activation, which normally occurs in response to early S phase arrest by nucleotide deprivation, is largely impaired in hsk1-89. Furthermore, Cds1-dependent hyperphosphorylation of Dfp1 in response to hydroxyurea arrest is eliminated in hsk1-89, suggesting that sufficient activation of Hsk1-Dfp1 kinase is required for S phase entry and replication checkpoint signaling. hsk1-89 displays apparent defect in mitosis at 37°C leading to accumulation of cells with near 2C DNA content and with aberrant nuclear structures. These phenotypes are similar to those of rad21-K1 and are significantly enhanced in a hsk1-89 rad21-K1 double mutant. Consistent with essential roles of Rad21 as a component for the cohesin complex, sister chromatid cohesion is partially impaired in hsk1-89, suggesting a possibility that infrequent origin firing of the mutant may affect the cohesin functions during S phase. INTRODUCTIONDNA replication needs to be stringently regulated for cell growth and cell division to occur in a coordinated manner (Stillman, 1996). Initiation of DNA replication requires assembly of multiprotein complexes at chromosomal replication origins during late M to early G1 phase Newlon, 1997). This complex, termed prereplicative complex (preRC), includes ORC (Bell and Stillman, 1992), Cdc6 (Cocker et al., 1996), and MCM proteins (Tye, 1994;Kearsey et al., 1995;Chong et al., 1996;Donovan et al., 1997), which are conserved from yeasts to human. After cells pass Start, the preRC is activated and DNA synthesis is initiated at replication origins. This process is accompanied with dissociation of Cdc6 (Cocker et al., 1996;Tanaka et al., 1997) and of at least some components of the MCM complex from origins Aparicio et al., 1997;Kubota et al., 1997;Tanaka et al., 1997), resulting in a postreplicative complex (postRC), which is inactive until the next cell cycle. The firing of origins requires actions of at least two distinct serine/threonine kinases, namely, G1/S-specific CDK-Cyclin (Nasmyth, 1996;Stillman, 1996) and Cdc7-Dbf4 (Hartwell, 1971, 1973.Saccharomyces cerevisiae CDC7 encodes a serine/threonine protein kinase required for the onset of DNA replication (Hollingsworth et al., 1992;Jackso...

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“…This leads to an asymmetric separation of chromosomes, resulting in either aneuploidy or aploidy and cell death (Hirano et al, 1986;Samejima et al, 1993). Several cut mutants have been characterized; their gene products cover a broad range of functions, including components of the spindle pole body (Bridge et al, 1998) and the anaphase-promoting complex (Samejima and Yanagida, 1994a,b), enzymes involved in DNA topology (Hirano et al, 1986;Saka et al, 1994) and replication (Saka et al, 1994), and microtubule-dependent motors (Hagan and Yanagida, 1990). cut11 ϩ , on the other hand, appears to be an essential component of the mitotic spindle pole body as well as a component of the nuclear pore complex.…”

Section: Introductionmentioning

confidence: 99%

cut11⁺: A Gene Required for Cell Cycle-dependent Spindle Pole Body Anchoring in the Nuclear Envelope and Bipolar Spindle Formation inSchizosaccharomyces pombe

West

Vaisberg

Ding

et al. 1998

MBoC

157

197

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The "cut" mutants of Schizosaccharomyces pombe are defective in spindle formation and/or chromosome segregation, but they proceed through the cell cycle, resulting in lethality. Analysis of temperature-sensitive alleles of cut11 ϩ suggests that this gene is required for the formation of a functional bipolar spindle. Defective spindle structure was revealed with fluorescent probes for tubulin and DNA. Three-dimensional reconstruction of mutant spindles by serial sectioning and electron microscopy showed that the spindle pole bodies (SPBs) either failed to complete normal duplication or were free floating in the nucleoplasm. Localization of Cut11p tagged with the green fluorescent protein showed punctate nuclear envelope staining throughout the cell cycle and SPBs staining from early prophase to mid anaphase. This SPB localization correlates with the time in the cell cycle when SPBs are inserted into the nuclear envelope. Immunoelectron microscopy confirmed the localization of Cut11p to mitotic SPBs and nuclear pore complexes. Cloning and sequencing showed that cut11 ϩ encodes a novel protein with seven putative membrane-spanning domains and homology to the Saccharomyces cerevisiae gene NDC1. These data suggest that Cut11p associates with nuclear pore complexes and mitotic SPBs as an anchor in the nuclear envelope; this role is essential for mitosis. INTRODUCTIONAccurate chromosome segregation requires proper assembly and function of a mitotic spindle. The spindle is constructed from microtubules (MT) 1 whose polymerization is nucleated by the centrosome (reviewed in Kellogg et al., 1994), which is known in fungi as the spindle pole body (SPB) (reviewed in Snyder, 1994). Although these two organelles are structurally distinct, genetic and biochemical approaches have identified several common components of centrosomes and SPBs, including ␥-tubulin (reviewed in Kellogg et al., 1994), CDC31/centrin (reviewed in Schiebel and Bornes, 1995), and p34 cdc2 (Bailly et al., 1989; Raibowol et al., 1989). Thus, analyses of SPBs have been informative about centrosomes in general.Recent work has demonstrated that the SPB of Schizosaccharomyces pombe is a dynamic organelle, undergoing significant changes in morphology and cellular localization as cells progress through their growth and division cycle (Ding et al., 1997). The nature of these changes distinguishes the fission yeast centrosome from that of other organisms. For example, the SPBs of the budding yeast Saccharomyces cer- evisiae duplicate in G 1 and remain in the nuclear envelope through the entire cell cycle (Byers, 1981;. The fission yeast SPB, on the other hand, resides in the cytoplasm through most of interphase, where it duplicates during late G 2 . As the cell enters mitosis, the nuclear envelope invaginates beneath the SPB and forms an opening, or fenestra, into which the duplicated SPB settles. Each part of the double SPB initiates intranuclear MTs; then the two parts separate to lie in distinct fenestrae, bound to the polar ends of the spindle MTs. As anaphas...

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Fission yeast cut5 links nuclear chromatin and M phase regulator in the replication checkpoint control.

Cited by 135 publications

References 60 publications

Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint

Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint

Regulation of Initiation of S Phase, Replication Checkpoint Signaling, and Maintenance of Mitotic Chromosome Structures during S Phase by Hsk1 Kinase in the Fission Yeast

cut11⁺: A Gene Required for Cell Cycle-dependent Spindle Pole Body Anchoring in the Nuclear Envelope and Bipolar Spindle Formation inSchizosaccharomyces pombe

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Fission yeast cut5 links nuclear chromatin and M phase regulator in the replication checkpoint control.

Cited by 135 publications

References 60 publications

Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint

Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint

Regulation of Initiation of S Phase, Replication Checkpoint Signaling, and Maintenance of Mitotic Chromosome Structures during S Phase by Hsk1 Kinase in the Fission Yeast

cut11+: A Gene Required for Cell Cycle-dependent Spindle Pole Body Anchoring in the Nuclear Envelope and Bipolar Spindle Formation inSchizosaccharomyces pombe

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cut11⁺: A Gene Required for Cell Cycle-dependent Spindle Pole Body Anchoring in the Nuclear Envelope and Bipolar Spindle Formation inSchizosaccharomyces pombe