Fishing of β-amylase with a SAM of α-cyclodextrin-poly- (ethylene glycol) conjugate

Kitano, Hiromi; Miyamoto, Takashi; Kawasaki, Hideaki

doi:10.1016/j.jcis.2004.06.076

Cited by 7 publications

(2 citation statements)

References 44 publications

(50 reference statements)

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“…5(d) and (e)). Previously, the effective reduction of non-specific adsorption of ␤-amylase to the sensor chip modified with ␣-cyclodextrin-PEG brush could be achieved by the incubation of the sensor chip in an aqueous 2-hydroxyethyldisulfide (2-HEDS) solution [59]. The same treatment of the PMEMan brush-modified chip, however, did not reduce the absorption change in lysozyme system, probably due to a strong electrostatic attraction between the anionic Au colloid and the positively charged lysozyme molecule (pI 11.0) with a small size (14.3 kDa).…”

Section: Binding Of Lectin To Sugar Residues On Colloidsmentioning

confidence: 99%

Kinetic study on the binding of lectin to mannose residues in a polymer brush

Kitano

Takahashi

Mizukami

et al. 2009

Colloids and Surfaces B: Biointerfaces

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Section: Binding Of Lectin To Sugar Residues On Colloidsmentioning

confidence: 99%

Kinetic study on the binding of lectin to mannose residues in a polymer brush

Kitano

Takahashi

Mizukami

et al. 2009

Colloids and Surfaces B: Biointerfaces

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“…An optical biosensor chip (SCX 6 -modified colloidal gold on glass) was prepared using a methodology similar to that reported previously ( 38 , − . A glass substrate (thickness = 160 μm, 9.5 × 24 mm) was cleaned by sonication for 2 h in water containing a 3% detergent (Contaminon N, Wako Pure Chemicals) and washed thoroughly with water.…”

Section: Methodsmentioning

confidence: 99%

Inclusion of Bisphenols by a Self-Assembled Monolayer of Thiolated Calix[6]arene on a Gold Surface

Nakaji-Hirabayashi

Endô

Kawasaki

et al. 2005

Environ. Sci. Technol.

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The molecular recognition of various kinds of bisphenols (BPs) by a self-assembled monolayer (SAM) of thiolated calix[6]arene on a gold electrode was examined using cyclic voltammetry (CV). Based on the inhibitory effect of BPs on the inclusion of hydroquinone (HQ) as a probe bythe surface-confined calix[6]arene, the association constants (Kassoc) of BPs with the immobilized calix[6]arene were estimated. The Kassoc values for BPs with the SAM of thiolated hexasodium calix[6]arenehexasulfonic acid (thioSCX6) were much smaller than those in the free calix[6]arene derivative systems reported previously. The order of the Kassoc values for BPs with thioSCX6 was bisphenol A (BPA) > bisphenol S (BPS) > bisphenol B (BPB) > bisphenol F (BPF). The Kassoc values for BPA and BPS with thioSCX6 were larger than that for BPB, despite the larger hydrophobicity of BPB than that of BPA and BPS. This is probably because the inclusion phenomena in this system are not simply driven by the hydrophobic interaction, but are significantly affected by several steric and structural factors with immobilization of the host. Those are (1) a decrease in flexibility of the SCX6 cavity by the formation of SAM, (2) a decrease in inclusion ability of the SCX6 by the presence of Au surface just beneath it, and (3) a repulsion of hydrophobic guests bythe presence of sulfonate groups at the top of the SAM. Moreover, the association phenomena (adsorption and desorption processes) of a bisphenol with the SCX6 SAM were examined directly by using localized surface plasmon resonance spectroscopy.

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