Fish Species Identification By Thin Layer Polyacrylamide Gel Isoelectric Focusing: Collaborative Study

Abstract

A method for fish species identification by isoelectric focusing of sarcoplasmic proteins on LKB Ampholine PAGplates, pH 3.5–9.5, was collaboratively studied to determine whether photographs of protein patterns from authentic species could be used to identify unknown samples. Seven collaborators were sent 8 unknown samples in duplicate to identify from an 8 × 10 in. photograph of an Ampholine PAGplate showing standard protein patterns from 14 species of fish. The 7 collaborators identified the unknown samples with 93% accuracy. Eight of 14 monkfish samples were not identified correctly and showed protein patterns different from the protein patterns shown in the photographic standard. All of the incorrectly identified samples showed the same protein pattern. The other 7 unknown species were all correctly identified. The method has been adopted as official first action.

Flavor Profiles of 17 Species of North Atlantic Fish

“…Most samples were approximately 5 cm square with thickness determined by the specific test species ( swordfish were cut to approximately 2 cm thickness). Portions of the fillet trimmings, representing at least six different samples of the species, were taken to the Gloucester Laboratory, Northeast Fisheries Center,.for species identification using thin-layer isoelectric focusing (Lundstrom, 1980;1981).…”

Preliminary Studies on SDS‐PAGE and Isoelectric Focusing Identification of Sturgeon Sources of Caviar

“…IEF, which applies an electric field to separate charged protein molecules (Robyt and White, 1987), has been used to identify seafood species such as shrimp Wei et al, 1990), crab (Gangar, 1992), snappers (Huang et al, 1995), ocean perch, monkfish, pollock, etc. (Lundstrom, 1980). IEF provides reproducible protein patterns for differentiating related species with ≥93% reliability (Lundstrom, 1980).…”

“…(Lundstrom, 1980). IEF provides reproducible protein patterns for differentiating related species with ≥93% reliability (Lundstrom, 1980). Although most work has been conducted on muscle sarcoplasmic proteins, Keyvanfar et al (1988) used soluble oocyte proteins to compare and identify some European sturgeon species by IEF.…”

Capillary Zone Electrophoresis of Fish Muscle Sarcoplasmic Proteins

“…hA~~s1.s OF SARCOPLASMIC PROTEIN (SAR) of fish muscle is of major interest for species identification because of legal requirements, quality, and pricing policies. A variety of analytical methods have been employed to identify fish species including starch, disc, cellulose acetate or sodium dodecyl sulfate polyacrylamide gel electrophoresis, and thin layer isoelectric focusing (Learson, 1969;Lundstrom, 1979Lundstrom, , 1980Krzynowek and Wiggin, 1981;Hamilton, 1982;An et al, 1988An et al, , 1989aWei et al, 1990). High performance liquid chromatograpy (I-IPLC) has provided species profiles for raw fish (Ashoor and Knox, 1985;LeBlanc and LeBlanc, 1989;Rehbein, 1990;Armstrong et al, 1992).…”