Fish protein hydrolysate supplementation in plant protein based diets for tiger puffer (Takifugu rubripes) is an effective strategy of fish meal sparing

The expressions of two myostatin paralogs in turbot Scophthalmus maximus fasted for 2 weeks and fed diets containing graded levels of fish protein hydrolysate

“…The concentrations of extracted RNA samples were measured using Colibri Microvolume Spectrophotometer (Titertek Berthold, Germany). The synthesis of cDNA templates was conducted using PrimeScript™ RT reagent kit with gDNA Eraser (TaKaRa, Japan) as described in our previous study (Wei et al, 2021). Genomic DNA from RNA sample was removed at 42°C for 2 min, cDNA templates were generated at 37°C for 15 min and reverse transcriptase was inactivated at 85°C for 5 s. A three‐step quantitative real‐time PCR (qRT‐PCR) was performed in a Roche LightCycler 96 system (Roche, Switzerland) for 40 cycles of denaturation at 95°C for 15 s, annealing at 58°C for 30s and extension at 72°C for 30s.…”

“…The beneficial effect of FPH on growth performance had been confirmed in many commercial fish including turbot ( Scophthalmus maximus ) (Hevrøy et al, 2005; Mamauag & Ragaza, 2017; Wei et al, 2020, 2021). Therefore, FPH supplementation in plant protein‐based diets had been considered as a promising way to solve the problem of fish meal shortage (Wei et al, 2021; Xu et al, 2016). Meanwhile, our previous study suggested that FPH showed a positive effect on growth muscle in turbot by down‐regulating the expression of MSTNb (Wei et al, 2020).…”

“…For more efficient utilization of fish by-products, they could be hydrolysed into fish protein hydrolysate (FPH) rich in peptides and free amino acids (Halim et al, 2016;Zamora-Sillero et al, 2018). The beneficial effect of FPH on growth performance had been confirmed in many commercial fish including turbot (Scophthalmus maximus) (Hevrøy et al, 2005;Mamauag & Ragaza, 2017;Wei et al, 2020Wei et al, , 2021.…”

“…Therefore, FPH supplementation in plant protein-based diets had been considered as a promising way to solve the problem of fish meal shortage (Wei et al, 2021;Xu et al, 2016). Meanwhile, our previous study suggested that FPH showed a positive effect on growth muscle in turbot by down-regulating the expression of MSTNb (Wei et al, 2020).…”

Application of poultry by‐product meal in diets of obscure pufferfish ( Takifugu obscurus )

“…The conditions of measurement included a 25N load cell, 8 mm cylinder probe, 30% deformation rate and a double cycle at a constant rate of 30 mm/min. TPA parameters were hardness, cohesiveness, springiness, gumminess, chewiness and adhesiveness, which were provided by the instrument software (Wei et al, 2021).…”