Fish cell lines as a tool in aquatic toxicology

Segner, Helmut

doi:10.1007/978-3-0348-8853-0_1

Cited by 71 publications

(70 citation statements)

References 142 publications

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“…Thus, the replacement or reduction of in vivo fish tests by in vitro cytotoxicity tests (Braunbeck and Strmac 2001, Segner 1998, Strmac and Braunbeck 2000 as well as embryo assays (Ensenbach 1998, Strmac et al 2002 have repeatedly been recommended. Given the high correlation with data from the fish test, both the cytotoxicity test and the fish embryo assay may provide good estimates of the potential toxicity of sediment-bound contaminants.…”

Section: Comparison Of Embryo Toxicity and Acute Toxicity In The Permmentioning

confidence: 99%

“…Acute cytotoxicity has frequently been investigated with the epithelial-like cell lines RTL-W1 (Lee et al 1993) and RTG-2 (Wolf and Quimby 1962, Zahn et al 1996) (both obtained from Oncorhynchus mykiss) in combination with the neutral red (Borenfreund and Puerner 1984) and succinic acid dehydrogenase (MTT) (Denizot and Lang 1986) assays, lactatedehydrogenase release into the medium (Weishaar et al 1975), as well as light microscopic inspection (Hollert et al 2000a). With these cell lines, acute cytotoxicity tests have sucessfully been used to determine the cytotoxicity of pure substance (Ekwall et al 1998, Segner 1998, Segner et al 1994, seewage waters from garbage dumps (Zahn et al 1995), waste waters (Castano et al 1994) and sediments (Hollert et al 2000a, b).…”

Section: Comparison Of Embryo Toxicity and Acute Toxicity In The Permmentioning

confidence: 99%

“…Thus, the replacement or reduction of in vivo fish tests by in vitro cytotoxicity tests (Segner 1998), as well as embryo assays (Ensenbach 1998) are recommended.…”

Section: Conclusion and Recommendationsmentioning

confidence: 99%

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A new sediment contact assay to assess particle-bound pollutants using zebrafish (danio rerio) embryos

Hollert

Keiter

König

et al. 2003

J Soils & Sediments

201

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A new sediment contact assay to assess particle-bound pollutants using zebrafish (Danio rerio) embryos. Soils and Sediments, http://dx.doi.org/10.1065/jss2003.09.085 Journal ofAccess to the published version may require subscription. Conclusions.A modified protocol of the zebrafish embryo test allows the assessment of sediment toxicity in both aqueous extracts and native sediments. The isolated investigation of pore waters may result in a clear-cut underestimation of the bioavailability of lipophilic particle-bound substances (as determined by native sediments). Recommendations and Perspectives.The zebrafish embryo test with native (whole, solid phase) sediments appears very promising for the evaluation of the bioavailable fraction of lipophilic particle-bound substances and can therefore be recommended for the evaluation of vertebrate toxicity in tiered sediment test strategies and dredging directives such as the HABAB-WSV. Whereas acetone extracts may be tested as a rough estimation of embryotoxicity, native sediment samples will provide a more comprehensive and realistic insight into the bioavailable hazard potential. (2) to compare the hazard potential of solid phase sediments to the effects of corresponding pore waters and organic extracts in order to characterize the bioavailability of the particle-bound pollutants; and (3) to relatively evaluate the embryotoxic effects of sediments from the catchment areas of the rivers Rhine, Neckar and Danube. Keywords Methods (or Main Features).To investigate the toxicity of sediment samples on vertebrates, the standard embryo toxicity test with the zebrafish (Danio rerio; Hamilton-Buchanan 1922) according to DIN 38415-6 was modified with respect to exposure scheme and toxicological endpoints. Sediments from the catchment area of the Neckar River were assessed using pore waters, acetonic extracts and native sediments in order to get inside into the potential bioavailability of particle-bound pollutants. A comprehensive test protocol for the investigation of native sediments in the embryo toxicity test with the zebrafish is presented.Results and Discussion. The fish embryo assay with Danio rerio can be carried out with both aqueous and organic sediment extracts as well as native (whole, solid phase) sediment samples. Elongation of exposure time from 48 to up to 196 h significantly increased the mortality. Using the fish egg assay with native sediments, a broad range of embryotoxic effects could be elucidated, including clear-cut dose-response curves for the embryotoxic effects of contaminated sediments; in contrast, absence of embryotoxic effects could be demonstrated even for the highest test concentrations of unpolluted sediments. With native sediments, embryotoxicity was clearly higher than with corresponding pore waters, thus corroborating the view that -at least for fish eggs -the bioavailability of particle-bound lipophilic substances in native sediments is higher than generally assumed. The relative ranking of sediment toxicity was identical using both nati...

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Section: Comparison Of Embryo Toxicity and Acute Toxicity In The Permmentioning

confidence: 99%

Section: Comparison Of Embryo Toxicity and Acute Toxicity In The Permmentioning

confidence: 99%

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A new sediment contact assay to assess particle-bound pollutants using zebrafish (danio rerio) embryos

Hollert

Keiter

König

et al. 2003

J Soils & Sediments

201

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“…A recent rise in use of cultured fish cells for a wide variety of in vitro toxicity studies (Baksi and Frazier 1990;Babich and Borenfreund 1991a;Bois and Lee 1991;Pesonen and Andersson 1997;Segner 1998), has made these systems available for use in studies of interactions between AHR signaling and cell proliferation. By extending knowledge of such effects beyond the mammalian class, the shared and distinct features among taxa can be used to build hypotheses of AHR evolution and function in cell proliferation.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of action for aryl hydrocarbon receptor ligands in the PLHC-1 cell line

Hestermann¹

1999

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“…Pollution may affect their health and cause extinction. These harmful changes are often associated with physiological reactions, which might be used as bioindicators (Segner, 1998;Segner & Braunbeck, 1998). Some researchers indicated that cestodes, nematodes and acanthocephalans are able to accumulate considerable …….…”

Section: Introductionmentioning

confidence: 99%

Concentrations of selected heavy metals in Ligula intestinalis L., 1758 plerocercoids (Cestoda) compared to it host’s (Tinca tinca L., 1758) organs from Beyşehir Lake (Turkey)

Tekin-Özan

Barlas

2008

Helminthologia

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76 SummaryThe accumulation of heavy metals (Cu, Fe, Zn and Mn) was measured by atomic absorption spectrophotometry in some organs of tench (Tinca tinca L., 1758) and tissues of its parasite collected from Beyşehir Lake, and compared with the data from sediments and water. The Cu levels in L. intestinalis L., 1758 plerocercoids were 1.69 times, respectively, higher than those in fish gill. Zn level in L. intestinalis plerocercoids was 2.13 times higher than those in fish liver, was 5.53 times higher than that of the muscle and 3.33 times higher than that in the gill. Significant negative (for Mn) correlation was found between the quantity of heavy metals in water and tissues of L. intestinalis plerocercoids while there was significant negative (for Cu) correlation between the quantity the heavy metals in bottom sediment and tissues of L. intestinalis plerocercoids. This study supports the idea that cestodes aren't very useful to determine the heavy metal pollution in aquatic system when they are located in their intermediate host's body cavity.

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Fish cell lines as a tool in aquatic toxicology

Cited by 71 publications

References 142 publications

A new sediment contact assay to assess particle-bound pollutants using zebrafish (danio rerio) embryos

A new sediment contact assay to assess particle-bound pollutants using zebrafish (danio rerio) embryos

Mechanisms of action for aryl hydrocarbon receptor ligands in the PLHC-1 cell line

Concentrations of selected heavy metals in Ligula intestinalis L., 1758 plerocercoids (Cestoda) compared to it host’s (Tinca tinca L., 1758) organs from Beyşehir Lake (Turkey)

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