Fish Biliary Polycyclic Aromatic Hydrocarbon Metabolites Estimated by Fixed-Wavelength Fluorescence: Comparison with HPLC-Fluorescent Detection

Lin, Edith L.C.; Cormier, Susan M.; Torsella, Joni

doi:10.1006/eesa.1996.0077

Cited by 136 publications

(67 citation statements)

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“…site (RM 9.2), but both of these sites were significantly lower in both types of metabolites than the three unremediated downstream sites. Metabolite levels at RM 9.2 were within statewide reference levels established using Ohio EPA biological reference sites [19,28] and sites sampled in Ohio for the Regional Environmental Monitoring and Assessment Program [24].…”

Section: Bile Metabolitesmentioning

confidence: 99%

“…fluorescence detection [18] or by fixed wavelength fluorescence [19,20]. The specific objectives of the present study were to: (1) document ongoing exposure of select fish species to sediment contaminants; (2) establish biological conditions in the Little Scioto River by evaluating fish and macroinvertebrate communities; (3) evaluate surficial sediment and surface water chemical quality at stations in the Little Scioto River and at several tributary locations; and (4) determine the aquatic life use attainment status of the Little Scioto River with regard to the modified warmwater habitat (MWH) or warmwater habitat (WWH) aquatic life use designations codified in the Ohio Water Quality Standards [21].…”

Section: Introductionmentioning

confidence: 99%

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An integrated assessment of sediment remediation in a midwestern U.S. stream using sediment chemistry, water quality, bioassessment, and fish biomarkers

Meier

Snyder

Sigler

et al. 2013

Enviro Toxic and Chemistry

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Abstract-A comprehensive biological, sediment, and water quality study of the lower Little Scioto River near Marion, Ohio, USA, was undertaken to evaluate the changes or improvements in biotic measurements following the removal of creosote-contaminated sediment. The study area covered 7.5 river miles (RMs), including a remediated section between RMs 6.0 and 6.8. Fish and macroinvertebrate assemblages, fish biomarkers (i.e., polycyclic aromatic hydrocarbon [PAH] metabolite levels in white sucker [Castostomus commersoni] and common carp [Cyprinus carpio] bile and DNA damage), sediment chemistry, and water quality were assessed at five locations relative to the primary source of historical PAH contamination-upstream (RM 9.2), adjacent (RM 6.5), and downstream (RMs 5.7, 4.4, and 2.7). Overall, the biomarker results were consistent with the sediment PAH results, showing a pattern of low levels of PAH bile metabolites and DNA damage at the upstream (reference or background location), as well as the remediated section, high levels at the two immediate downstream sites, and somewhat lower levels at the furthest downstream site. Results show that remediation was effective in reducing sediment contaminant concentrations and exposure of fish to PAHs and in improving fish assemblages (60% increase in index of biotic integrity scores) in remediated river sections. Additional remedial investigation and potentially further remediation is needed to improve the downstream benthic fish community, which is still heavily exposed to PAH contaminants. Environ.

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Section: Bile Metabolitesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An integrated assessment of sediment remediation in a midwestern U.S. stream using sediment chemistry, water quality, bioassessment, and fish biomarkers

Meier

Snyder

Sigler

et al. 2013

Enviro Toxic and Chemistry

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“…The term ''metabolite-type'' is used as groups of compounds that fluoresce at specific wavelengths are detected. This offers the advantage of a particularly sensitive detection of a group of metabolites originating from a common parent compound (Lin et al, 1996). For example, nearly all ''B[a]P-type'' metabolites fluoresce at 380/430 nm (Lin et al, 1996) and ''pyrene-type'' metabolites fluoresce at 340/380 nm (Krahn et al, 1986).…”

Section: Bile Metabolite Measurementmentioning

confidence: 99%

“…This offers the advantage of a particularly sensitive detection of a group of metabolites originating from a common parent compound (Lin et al, 1996). For example, nearly all ''B[a]P-type'' metabolites fluoresce at 380/430 nm (Lin et al, 1996) and ''pyrene-type'' metabolites fluoresce at 340/380 nm (Krahn et al, 1986). The concentrations of PAH bile metabolites are reported as metabolite equivalents to their respective standards, representing the amount of a metabolite that would be present if the group of metabolites originated from a parent compound (Krahn et al, 1986).…”

Section: Bile Metabolite Measurementmentioning

confidence: 99%

“…Bile samples were thawed on ice and diluted to 1:4000 in 50% HPLC grade methanol/H 2 O for FF determination of pyrene-type metabolites and measured on a Perkin-Elmer LS-45 Luminescence Spectrometer at excitation/emission wavelengths of 340/380 nm (slit 10 ex/10 em) (Aas et al, 1998). The bile was further diluted to 1:8000 for the determination of B[a]P-type metabolites and read at excitation/ emission wavelengths of 380/430 nm (slit 10 ex/10 em) (Lin et al, 1996). The concentration of PAH metabolites in the bile was determined using 1-hydroxypyrene (98%; 1-OH pyrenol) as the external standard with the fluorescence reading of bile converted to 1-OH pyrenol equivalents from the linear regression curves (pyrene, 0.000 to 0.046 lM; B[a]P, 0.000 to 0.458 lM).…”

Section: Bile Metabolite Measurementmentioning

confidence: 99%

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Hepatic metabolism of contaminants in the terapontid fish, yellowtail trumpeter (Amniataba caudavittata Richardson)

Webb

Gagnon

Rose

2008

Environmental Toxicology

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The yellowtail trumpeter (Amniataba caudavittata) is an estuarine-dependent omnivorous fish found in the Swan-Canning Estuary, Western Australia. Thirty five fish were injected with either the polycyclic aromatic hydrocarbon, benzo[a]pyrene (B[a]P), the synthetic flavenoid b-naphthoflavone (BNF), or used as controls. The fish were then sampled at 3 and 7 days postinjection. Induction of the enzyme ethoxyresorufin O-deethylase (EROD) activity was nonsignificant while ethoxycoumarin O-deethylase (ECOD) activity induction differed amongst treatments. A high interindividual variability in the EROD activity was observed. The measurement of sorbitol dehydrogenase in the serum (s-SDH) was elevated (BNF 2.2 times and B[a]P 3.2 times the control fish) demonstrating that liver cell damage had occurred. Increases in biliary metabolites of both B[a]P-type and pyrene-type (19 times and 3.4 times the controls respectively) indicated that detoxification of pyrene-type compounds had taken place. Fish of the Terapontidae family, such as the yellowtail trumpeter, were found to be suitable for biomonitoring the health of the Swan-Canning Estuary. A combination of ECOD activity, s-SDH, and the measurement of biliary metabolites represents a suitable suite of biomarkers for environmental monitoring of the sublethal effects of PAH pollution in these fish. # 2008 Wiley Periodicals, Inc. Environ Toxicol 23: 68-76, 2008

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Improvements to the Environmental Performance of Synthetic‐Based Drilling Muds

Bakhtyar

Gagnon

2012

Handbook of Green Chemistry

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Drilling muds used by the petroleum exploration and production industry have the role of lubricating and cooling the drilling bits and facilitating the transport of crushed rocks to the surface of the well. The chemical formulations of the drilling muds influence their fate when discharged into the marine environment and, along with ambient parameters, determine the biodegradability and toxicity of the muds. A case study describes the comparison of aquatic toxicity and biodegradability of two different synthetic‐based drilling mud systems (SBMs), Syndrill 80:20 and Syndrill 90:10, and also individual mud ingredients. Chronic fish toxicity was measured using a suite of biomarkers of fish health. Preliminary tests led to the selection of the drilling mud Syndrill 80:20, and toxicity testing of individual ingredients of this mud showed that the primary emulsifier was the most aggressive component among all the mud ingredients tested. The present study aimed at developing an ecologically safe, green SBM by further improvements to Syndrill 80:20 through replacement of the most aggressive emulsifier with an alternative low/non‐toxic alternative. The existing Syndrill 80:20 formulation was re‐engineered by the incorporation of two alternative emulsifiers as replacements for the existing aggressive emulsifier. The new modified Syndrill 80:20 was then evaluated for its chronic toxicity against the existing Syndrill 80:20 using a suite of biomarkers of fish health. Following exposure to the modified mud system, pink snapper ( Pagrus auratus ) demonstrated weaker biological reactions as measured by ethoxyresorufin‐ O ‐deethylase (EROD) activity, biliary metabolites, and serum sorbitol dehydrogenase (SDH) activity. Closed‐bottle biodegradation tests also indicated that the new formulation is actively biodegrading under marine anaerobic conditions. Therefore, the information on the toxicity of the re‐engineered SBM provides an initial basis for evaluating its environmental performance in marine ecosystems, and may assist regulatory authorities in gaining a better understanding of the fate of SBMs in the marine environment.

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Fish Biliary Polycyclic Aromatic Hydrocarbon Metabolites Estimated by Fixed-Wavelength Fluorescence: Comparison with HPLC-Fluorescent Detection

Cited by 136 publications

References 0 publications

An integrated assessment of sediment remediation in a midwestern U.S. stream using sediment chemistry, water quality, bioassessment, and fish biomarkers

An integrated assessment of sediment remediation in a midwestern U.S. stream using sediment chemistry, water quality, bioassessment, and fish biomarkers

Hepatic metabolism of contaminants in the terapontid fish, yellowtail trumpeter (Amniataba caudavittata Richardson)

Improvements to the Environmental Performance of Synthetic‐Based Drilling Muds

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