Fish as model in pharmacological and biological research

Bolis, C. L.; Piccolella, Margherita; Valle, Andrea Zenone Dalla; Rankin, J. C.

doi:10.1006/phrs.2001.0845

Cited by 51 publications

(27 citation statements)

References 129 publications

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“…When introduced into the aquatic environment, they may affect lower animals with identical or similar target organs, tissues, cells or biomolecules (Fent et al, 2006). Nevertheless, certain receptors in lower animals resembling those in humans are different or are completely lacking, which means that dissimilar modes of actions may occur (Bolis et al, 2001;Bound and Voulvoulis, 2004).…”

Section: Toxicity Of Residue Phacs On Aquatic Organismsmentioning

confidence: 99%

Residual pharmaceutically active compounds (PhACs) in aquatic environment - status, toxicity and kinetics: a review

Li¹,

Randák²

2009

Vet. Med.

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Awareness of residual pharmaceutically active compounds (PhACs) in aquatic ecosystems is growing as research into these pollutants increases and analytical detection techniques improve. For most pharmaceuticals analyzed, the effects on aquatic organisms have usually been investigated by toxic assays in the laboratory. However, little is known about integral analysis of pharmacokinetics in aquatic organisms and specific relations between pharmacokinetic parameters and influence factors. Moreover, the influence of the organisms involved and numerous other external factors complicates development of standard tests for environmental evaluation. Current knowledge about residual pharmaceuticals in the aquatic environment, including status, toxic effects, and pharmacokinetics in aquatic organisms, are reviewed. Based on the above, we identify major gaps in the current knowledge and some directions for future research, such as improvement of techniques to remove residual pharmaceuticals from wastewater, and the establishment of standard pharmaceutical modes of action.

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Section: Toxicity Of Residue Phacs On Aquatic Organismsmentioning

confidence: 99%

Residual pharmaceutically active compounds (PhACs) in aquatic environment - status, toxicity and kinetics: a review

Li¹,

Randák²

2009

Vet. Med.

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“…The relative abundance and extensive biochemical and genetic characterization of in vitro cell systems in mammals contrasts singularly with the information available for other vertebrate organisms and particularly fish, the most diverse and oldest class of vertebrates (Bolis et al 2001). Fish, by sharing with mammals a large number of important characteristics (e.g.…”

Section: Introductionmentioning

confidence: 98%

Establishment of primary cell cultures from fish calcified tissues

et al. 2007

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Fishes have been recently recognized as a suitable model organism to study vertebrate physiological processes, in particular skeletal development and tissue mineralization. However, there is a lack of well characterized in vitro cell systems derived from fish calcified tissues. We describe here a protocol that was successfully used to develop the first calcified tissue-derived cell cultures of fish origin. Vertebra and branchial arches collected from young gilthead seabreams were fragmented then submitted to the combined action of collagenase and trypsin to efficiently release cells embedded in the collagenous extracellular matrix. Primary cultures were maintained under standard conditions and spontaneously transformed to form continuous cell lines suitable for studying mechanisms of tissue mineralization in seabream. This simple and inexpensive protocol is also applicable to other calcified tissues and species by adjusting parameters to each particular case.

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“…Fish are aquatic vertebrates that use their gills to breathe, and mammals are vertebrates that breathe using their lungs. Nevertheless, there are important similarities between fish and mammals in the neuroanatomical networks and nervous mechanisms that control the ventilatory and cardiovascular systems (Taylor et al, 1999, 2010a,b; Bolis et al, 2001). In addition, neuropeptides appeared very early during evolution and the primary structures of these peptides and their receptors have been generally well conserved from fish to mammals (Holmgren and Jensen, 2001).…”

Section: Introductionmentioning

confidence: 99%

Brain neuropeptides in central ventilatory and cardiovascular regulation in trout

et al. 2012

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Many neuropeptides and their G-protein coupled receptors (GPCRs) are present within the brain area involved in ventilatory and cardiovascular regulation but only a few mammalian studies have focused on the integrative physiological actions of neuropeptides on these vital cardio-respiratory regulations. Because both the central neuroanatomical substrates that govern motor ventilatory and cardiovascular output and the primary sequence of regulatory peptides and their receptors have been mostly conserved through evolution, we have developed a trout model to study the central action of native neuropeptides on cardio-ventilatory regulation. In the present review, we summarize the most recent results obtained using this non-mammalian model with a focus on PACAP, VIP, tachykinins, CRF, urotensin-1, CGRP, angiotensin-related peptides, urotensin-II, NPY, and PYY. We propose hypotheses regarding the physiological relevance of the results obtained.

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Fish as model in pharmacological and biological research

Cited by 51 publications

References 129 publications

Residual pharmaceutically active compounds (PhACs) in aquatic environment - status, toxicity and kinetics: a review

Residual pharmaceutically active compounds (PhACs) in aquatic environment - status, toxicity and kinetics: a review

Establishment of primary cell cultures from fish calcified tissues

Brain neuropeptides in central ventilatory and cardiovascular regulation in trout

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