Fisetin inhibits the growth and migration in the A549 human lung cancer cell line via the ERK1/2 pathway

Wang, Junjian; Huang, Shangxiang

doi:10.3892/etm.2017.5666

Cited by 28 publications

(33 citation statements)

References 31 publications

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“…After short-term EGCG, the exposure of lung cancer cells was observed in that EGF-induced EGFR, Akt and ERK1/2 activation was substantially decreased [45]. In vitro studies demonstrated that fisetin inhibited the growth and migration of non-small cell lung cancer by inhibiting the activation of the ERK signaling pathway via MEK1/2 [46].…”

Section: Flavonoids As Key Gene Expression Regulators In Lung Cancermentioning

confidence: 99%

Progress in Research on the Role of Flavonoids in Lung Cancer

Zănoagă

Braicu

Jurj

et al. 2019

IJMS

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Lung cancer is the leading cause of cancer deaths worldwide. Therefore, for the prevention, diagnosis, prognosis and treatment of lung cancer, efficient preventive strategies and new therapeutic strategies are needed to face these challenges. Natural bioactive compounds and particular flavonoids compounds have been proven to have an important role in lung cancer prevention and of particular interest is the dose used for these studies, to underline the molecular effects and mechanisms at a physiological concentration. The purpose of this review was to summarize the current state of knowledge regarding relevant molecular mechanisms involved in the pharmacological effects, with a special focus on the anti-cancer role, by regulating the coding and non-coding genes. Furthermore, this review focused on the most commonly altered and most clinically relevant oncogenes and tumor suppressor genes and microRNAs in lung cancer. Particular attention was given to the biological effect in tandem with conventional therapy, emphasizing the role in the regulation of drug resistance related mechanisms.

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Section: Flavonoids As Key Gene Expression Regulators In Lung Cancermentioning

confidence: 99%

Progress in Research on the Role of Flavonoids in Lung Cancer

Zănoagă

Braicu

Jurj

et al. 2019

IJMS

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“…Several studies have investigated the effect of fisetin on NSCLC cells. In general, it was observed that this flavonoid inhibited adhesion, migration and invasion (4,48) by downregulating the expression of MMP-2, u-PA, Twist, N-cadherin, and c-Jun and upregulating E-cadherin and ZO-1. It was reported that fisetin inhibited the phosphorylation of ERK1/2 and also downregulated proteins involved in the induction of EMT, such as STAT-3, EGFR, and NF-kB (28,86).…”

Section: Flavonoidsmentioning

confidence: 95%

“…Lastly, other complementary methodologies have been used to characterize invasiveness, such as the cell matrixadhesion assay (26,28,46,48). This assay consists of the quantification of cells adherent to a specific substrate, usually type I collagen.…”

Section: Invasion Assaysmentioning

confidence: 99%

A narrative review of the migration and invasion features of non-small cell lung cancer cells upon xenobiotic exposure: insights from in vitro studies

Albuquerque¹,

Manguinhas²,

Costa³

et al. 2021

Transl Lung Cancer Res

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Lung cancer (LC) is the leading cause of cancer deaths worldwide, being non-small lung cancer (NSCLC) sub-types the most prevalent. Since most LC cases are only detected during the last stage of the disease the high mortality rate is strongly associated with metastases. For this reason, the migratory and invasive capacity of these cancer cells as well as the mechanisms involved have long been studied to uncover novel strategies to prevent metastases and improve the patients' prognosis. This narrative review provides an overview of the main in vitro migration and invasion assays employed in NSCLC research. While several methods have been developed, experiments using conventional cell culture models prevailed, specifically the wound-healing and the transwell migration and invasion assays. Moreover, it is provided herewith a summary of the available information concerning chemical contaminants that may promote the migratory/ invasive properties of NSCLC cells in vitro, shedding some light on possible LC risk factors. Most of the reported agents with pro-migration/invasion effects derive from cigarette smoking [e.g., Benzo(a)pyrene and cadmium] and air pollution. This review further presents several studies in which different dietary/ plant-derived compounds demonstrated to impair migration/invasion processes in NSCLC cells in vitro.These chemicals that have been proposed as anti-migratory consisted mainly of natural bioactive substances, including polyphenols non-flavonoids, flavonoids, bibenzyls, terpenes, alkaloids, and steroids. Some of these compounds may eventually represent novel therapeutic strategies to be considered in the future to prevent metastasis formation in LC, which highlights the need for additional in vitro methodologies that more closely resemble the in vivo tumor microenvironment and cancer cell interactions. These studies along with adequate in vivo models should be further explored as proof of concept for the most promising compounds.

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“…Fisetin inhibited cell migration and invasion of cervical cancer cells by repressing uPA via interruption of the p38 MAPK-dependent NF-ĸB signaling pathway (25). Recently, it was also reported that fisetin inhibited the cell growth and migration of human lung cancer A549 cells by blocking the ERK1/2 pathway (26). However, there is no available information showing the effect of fisetin in the cell migration and invasion of human osteosarcoma cells, which was therefore the aim of the present study.…”

mentioning

confidence: 96%

Fisetin Suppresses Human Osteosarcoma U-2 OS Cell Migration and InvasionviaAffecting FAK, uPA and NF-ĸB Signaling PathwayIn Vitro

Chen

Peng

Lai

et al. 2019

In Vivo

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Background/Aim: Evidence has indicated that fisetin induces cytotoxic effects in human cancer cell lines, including the inhibition of cell migration and invasion, however, the exact molecular mechanism of action of fisetin in human osteosarcoma cells remains unclear. Materials and Methods: The anti-metastatic mechanisms of fisetin in human osteosarcoma U-2 OS cells were investigated in vitro. Results: Fisetin reduced the viability of cells at different concentrations (2.5, 5 and 10 μM) as measured by flow cytometric assay. Fisetin suppressed cell mobility, migration and invasion of U-2 OS cells, as shown by wound healing assay and transwell filter chambers, respectively. The gelatin zymography assay showed that fisetin inhibited MMP-2 activity in U-2 OS cells. Results from western blotting indicated that fisetin reduced the levels

show abstract

Fisetin inhibits the growth and migration in the A549 human lung cancer cell line via the ERK1/2 pathway

Cited by 28 publications

References 31 publications

Progress in Research on the Role of Flavonoids in Lung Cancer

Progress in Research on the Role of Flavonoids in Lung Cancer

A narrative review of the migration and invasion features of non-small cell lung cancer cells upon xenobiotic exposure: insights from in vitro studies

Fisetin Suppresses Human Osteosarcoma U-2 OS Cell Migration and InvasionviaAffecting FAK, uPA and NF-ĸB Signaling PathwayIn Vitro

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