Fischoederius cobboldi: A scanning electron microscopy investigation of surface morphology of adult rumen fluke

Anuracpreeda, Panat; Panyarachun, Busaba; Ngamniyom, Arin; Tinikul, Yotsawan; Chotwiwatthanakun, Charoonroj; Poljaroen, Jaruwan; Sobhon, Prasert

doi:10.1016/j.exppara.2012.02.001

Cited by 20 publications

(18 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was in contrast to the testes of both F. hepatica [7] and F. gigantica [2] that are located side by side in the central part of the body. In general, spermatogenesis in P. gracile is similar to that of other digenean parasites in that spermatogonia undergo three mitotic and two meiotic divisions to give rise to 32 spermatids that are transformed into 32 spermatozoa [11,29].…”

Section: Discussionmentioning

confidence: 72%

“…The histological components of the body wall consisting of the tegument, basement membrane, tegumental cells, and muscle layers were similar to those of other closely related trematode parasites such as Fasciola gigantica [2,4]. The anterior sucker was found at an anterior location while the posterior sucker (acetabulum) was present on the posterior tip similar to many species of rumen flukes including Paramphistomum ichikawai , P. bombayiensis , Calicophoron ( C. ) crassum , C. calicophorum , C. cauliorchis [9], and Fischoederius cobboldi [3].…”

Section: Discussionmentioning

confidence: 91%

See 1 more Smart Citation

Morphology and histology of the adultParamphistomum gracileFischoeder, 1901

et al. 2013

Self Cite

View full text Add to dashboard Cite

In the present study, we evaluated the histological morphology of the adult Paramphistomum (P.) gracile. Adult flukes with bodies 5~15 mm in length and 2~7 mm in width were subjected to histological analysis. Longitudinal and transversal serial-sections were stained with hematoxylin and eosin, and examined. The body surface and longitudinal section of P. gracile were also assessed using scanning electron microscopy. In this species, the anterior sucker and posterior sucker (acetabulum) were present on an anterior and posterior part of the body, respectively. The major folds were located in the areas of the anterior sucker, genital canal, and posterior sucker. The fluke membrane was spineless at the tegument surface and in the tegument tissue. Histological data showed structural-systematic characteristics of the digestive tract, reproductive tract, excretory tract, copulatory organs, connective tissues, and muscle tissues. We attempted to elucidate the histological characteristics of P. gracile that might increase the knowledge and understanding of rumen fluke morphology.

show abstract

Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 91%

Morphology and histology of the adultParamphistomum gracileFischoeder, 1901

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Paramphistomes are trematode parasites of the family Paramphistomatidae, also known as stomach fluke, infecting the rumen and reticulum of ruminants, including roe deer . Migration of the immature parasites from the small intestine towards the rumen and reticulum causes acute parasitic gastroenteritis especially in young ruminants , while adult parasites establish in the forestomach and is the main cause of chronic ulcerative rumenitis . The disease, paramphistomosis, is an important cause of productivity and economic losses characterized by sporadic epizootics throughout tropical and subtropical regions, particularly in Africa, Asia, Australia, Eastern Europe and Russia .…”

Section: Introductionmentioning

confidence: 99%

Antigenic profile, isolation and characterization of whole body extract of Paramphistomum gracile

Anuracpreeda

Chawengkirttikul

Sobhon

2016

Parasite Immunology

View full text Add to dashboard Cite

An antigenic component of adult Paramphistomum gracile was characterized by means of indirect enzyme-linked immunosorbent assay (indirect ELISA), sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting using sera from cattle naturally infected with P. gracile, Eurytrema pancreaticum, Fasciola gigantica, Moniezia benedeni, strongylids, Trichuris sp. and Strongyloides sp. The whole body (WB) extracts of P. gracile were fractionated by gel filtration chromatography in a Sephadex G-200 column. It was found that the WB extract fractions, F1-F3 were highly antigenic, F5 was moderately antigenic and F4 was poorly antigenic. For SDS-PAGE and immunoblotting, the antigenic molecules of WB extract and all five fractions were mostly at molecular weights (MW) ranging from 12 to 150 kDa. One antigenic protein of 16 kDa detected in WB extract and F1-F3 was found to give a consistent reaction with sera from infected cattle. The antigenicity of the purified 16 kDa protein was confirmed by immunoblotting and indirect ELISA using a pool of sera and individual serum samples from infected cattle (at 1 : 78 125 dilution) and hyperimmunized rabbit (at 1 : 390 625 dilution). This finding suggests that the 16 kDa protein may be a potential antigen for the immunodiagnosis of cattle paramphistomosis caused by P. gracile.

show abstract

“…cattle, buffaloes, goats and sheep, which is caused by digenetic flukes belong to the superfamily of Paramphistomoidea (Gupta et al 1978). This disease is found in many regions, particularly in Africa, Asia, Australia, eastern Europe and Russia, which results in major economic losses in agriculture (Gupta et al 1978; Nikitin, 1979; Hanna et al 1988; Anuracpreeda et al 2008, 2012, 2015). The occurrence of this disease, mainly with Paramphistomum gracile , has also been reported (Chethanon et al 1985; Prasitirat et al 1997; Panyarachun et al 2013; Anuracpreeda et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Immunodiagnosis of paramphistomosis using monoclonal antibody-based sandwich ELISA for detection ofParamphistomum gracilecirculating 16 kDa antigen

2017

Self Cite

View full text Add to dashboard Cite

In this study, we have produced a monoclonal antibody (MoAb) against 16 kDa antigen of Paramphistomum gracile (16 kDaAgPg), and developed an accurate sandwich enzyme-linked immunosorbent assay (sandwich ELISA) for the detection of circulating 16 kDaAg in the serum and fecal samples from cattle naturally infected with P. gracile. MoAb 1D10 was immobilized on a microtitre plate, and the antigen in the samples was captured and detected with biotinylated rabbit anti-16 kDaAgPg antibody. The lower detection limit of sandwich ELISA was 3·5 pg mL-1, and no cross-reaction with other parasite antigens was evaluated. The reliability of the assay was examined using the serum and fecal samples from cattle naturally infected with P. gracile, Fasciola gigantica, Moniezia benedeni, Trichuris sp., Strongyloides sp., strongylids and non-infected animals. The sandwich ELISA showed the sensitivity, specificity and accuracy at 98·33, 100 and 99·55% (serum samples), and 96·67, 100 and 99·09% (fecal samples). Therefore, this detection method is a rapid and excellent potential assay for the accurate diagnosis of paramphistomosis.

show abstract

Fischoederius cobboldi: A scanning electron microscopy investigation of surface morphology of adult rumen fluke

Cited by 20 publications

References 20 publications

Morphology and histology of the adultParamphistomum gracileFischoeder, 1901

Morphology and histology of the adultParamphistomum gracileFischoeder, 1901

Antigenic profile, isolation and characterization of whole body extract of Paramphistomum gracile

Immunodiagnosis of paramphistomosis using monoclonal antibody-based sandwich ELISA for detection ofParamphistomum gracilecirculating 16 kDa antigen

Contact Info

Product

Resources

About