Morphology and histology of the adultParamphistomum gracileFischoeder, 1901

Abstract

In the present study, we evaluated the histological morphology of the adult Paramphistomum (P.) gracile. Adult flukes with bodies 5~15 mm in length and 2~7 mm in width were subjected to histological analysis. Longitudinal and transversal serial-sections were stained with hematoxylin and eosin, and examined. The body surface and longitudinal section of P. gracile were also assessed using scanning electron microscopy. In this species, the anterior sucker and posterior sucker (acetabulum) were present on an anterior and posterior part of the body, respectively. The major folds were located in the areas of the anterior sucker, genital canal, and posterior sucker. The fluke membrane was spineless at the tegument surface and in the tegument tissue. Histological data showed structural-systematic characteristics of the digestive tract, reproductive tract, excretory tract, copulatory organs, connective tissues, and muscle tissues. We attempted to elucidate the histological characteristics of P. gracile that might increase the knowledge and understanding of rumen fluke morphology.

Antigenic profile, isolation and characterization of whole body extract of Paramphistomum gracile

“…Active adult P. gracile was collected from the rumen of infected cattle slaughtered for consumption at the local abattoir, Pathum Thani Province, Thailand. They were then washed several times with 0·85% NaCl solution and used immediately .…”

“…The method described by Anuracpreeda et al . was used to obtain WB extract. Briefly, adult parasites were homogenized in lysis buffer (0·01 m phosphate buffer saline (PBS), pH 7·2, containing 10 m m Tris–HCl, 150 m m NaCl, 0·5% Triton X‐100, 10 m m EDTA and 1 m m PMSF), and then sonicated for 5 min in an ice bath with 15‐s pulses.…”

“…Paramphistomes are trematode parasites of the family Paramphistomatidae, also known as stomach fluke, infecting the rumen and reticulum of ruminants, including roe deer . Migration of the immature parasites from the small intestine towards the rumen and reticulum causes acute parasitic gastroenteritis especially in young ruminants , while adult parasites establish in the forestomach and is the main cause of chronic ulcerative rumenitis .…”

The complete mitochondrial genome of Gastrothylax crumenifer (Gastrothylacidae, Trematoda) and comparative analyses with selected trematodes

“…It is reported that Gastrothylax crumenifer, Paramphistomum cervi, and Paramphistomum epiclitum are common in ruminants in some regions (Gupta et al 1987;Panyarachun et al 2013;Wang et al 2006). G. crumenifer is an important member of paramphistomes parasitizing the rumen and/or reticulum of ruminants.…”

Molecular Characterization of Gastrothylax crumenifer (Platyhelminthes: Gastrothylacidae) from Goats in the Western Part of India by LSU of Nuclear Ribosomal DNA

“…The prevalence of this parasite is very high in tropical countries [ 4 , 5 ]. Some reports specified that, in tropical and subtropical areas, Paramphistomum cervi , P. Epiclitum , and G. crumenifer are the most prevalent parasites which cause the disease in ruminants [ 6 , 7 , 8 ].…”