First report on extended-spectrum beta-lactamase (ESBL) producing Escherichia coli from European free-tailed bats (Tadarida teniotis) in Portugal: A one-health approach of a hidden contamination problem

Garcês, Andreia; Correia, Susana; Amorim, Francisco; Pereira, José Eduardo; Igrejas, Gilberto; Poeta, Patrícia

doi:10.1016/j.jhazmat.2017.12.053

Cited by 18 publications

(22 citation statements)

References 47 publications

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“…In our samples, the highest prevalence of resistance was obtained in resistance to aminoglycosides (kanamycin, tobramycin and streptomycin), fluoroquinolones (ciprofloxacin and nalidixic acid) and tetracycline, which are the most common antibiotics consumed by the Gabonese people [21,54]. The congruence between the resistance prevalence and the frequency of antibiotics consumed by local people was also observed in Portugal [12] but not in Nigeria [15]. The low percentage of carbapenem resistance in our study suggests that these antibiotics are still very active against multiresistant strains [55].…”

Section: Discussionsupporting

confidence: 65%

“…The CTX-M-15-producing K. pneumoniae was found in a gorilla in its natural habitat in the Dzanga Ndoki National Park [27]. CTX-M-15 is the most highly detected genotype in human clinical settings [45,46] and in Tadarida teniotis (bats) in Portugal [12]. Enterobacteria producing ESBL have already been found on all continents, and the most frequently encountered is E. coli, followed by K. pneumoniae [47].…”

Section: Discussionmentioning

confidence: 99%

“…In several studies, antibiotic-resistant bacteria have been described in bat isolates, suggesting the possibility of these mammals being one of their environmental reservoirs [4,6,[9][10][11][12][13][14][15]. In a survey of wild and captive grey-headed flying foxes (Pteropus poliocephalus), McDougall et al detected the presence of resistance to several antibiotic families in 5.3% of wild flying foxes from different Australian colonies [6].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of ESBL-Producing Enterobacteria from Fruit Bats in an Unprotected Area of Makokou, Gabon

et al. 2020

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In Gabon, terrestrial mammals of protected areas have been identified as a possible source of antibiotic-resistant bacteria. Some studies on antibiotic resistance in bats have already been carried out. The main goal of our study was to detect extended-spectrum beta-lactamases (ESBLs) that are produced by enterobacteria from bats in the Makokou region in Gabon. Sixty-eight fecal samples were obtained from 68 bats caught in the forests located 1 km from the little town of Makokou. After culture and isolation, 66 Gram-negative bacterial colonies were obtained. The double-disk diffusion test confirmed the presence of ESBLs in six (20.69%) Escherichia coli isolates, four (13.79%) Klebsiella pneumoniae isolates, and one (3.45%) Enterobacter cloacae isolate. The analysis based on the nucleotide sequences of the ESBL resistance genes showed that all cefotaximase-Munichs (CTX-Ms) were CTX-M-15 and that all sulfhydryl variables (SHVs) were SHV-11: 41.67% CTX-M-15-producing E. coli, 16.67% CTX-M-15+SHV-11-producing E. coli, 8.33% CTX-M-15-producing K. pneumoniae, 25% CTX-M-15+SHV-11-producing K. pneumoniae, and 8.33% CTX-M-15-produced E. cloacae. This study shows for the first time the presence of multiresistant ESBL-producing enterobacteria in fruit bats in Makokou.

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Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of ESBL-Producing Enterobacteria from Fruit Bats in an Unprotected Area of Makokou, Gabon

et al. 2020

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“…The spread worldwide of ESBL Enterobacteriaceae clinical isolates is a serious problem for the treatment of infectious diseases, in particular the emergence of E. coli producing CTX-M-15 [ 16 ]. CTX-M-15 is probably the most widespread ESBL gene in humans worldwide [ 55 ] and the most detected in human clinical contexts [ 56 ]. In addition, the CTX-M-15 and SHV-11 genes are recognized as plasmid-mediated resistance genes [ 42 , 57 ].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Extended-Spectrum β-Lactamases in E. coli of Rats in the Region North East of Gabon

Onanga

Nguema

Atome³

et al. 2020

Veterinary Medicine International

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Antibiotic resistance occurs in the environment by multiplication and the spread of multidrug-resistant bacteria that would be due to an improper and incorrect use of antibiotics in human and veterinary medicine. The aim of this study was to establish the prevalence of E.coli producing Extended-Spectrum beta-Lactamase (ESBL) antibiotics from rats and gregarious animals in a semirural area of Gabon and to evaluate the origin of a resistance distribution in the environment from animal feces. The bacterial culture was carried out, and the identification of E. coli strains on a specific medium and the antibiotic susceptibility tests allowed establishing the prevalence. Characterization of resistance genes was performed by gene amplification after DNA extraction. On 161 feces collected in rats, 32 strains were isolated, and 11 strains of E. coli produced ESBL with a prevalence of 34.37%. Molecular tests showed that CTX-M genes 214 bp were identified in rats. The presence of CTX-M genes could have a human origin. So, the rats can carry ESBL-producing Enterobacteriaceae which poses a risk to human health and pets in this region of Gabon.

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“…Interestingly, Hasan et al (2016) reported that among five different bird species evaluated, cattle egrets, which forage at the feet of grazing cattle, showed the highest prevalence of ESBL-producing bacteria, suggesting a high probability of transmission of ARMs between birds and cattle in the environment where they co-inhabit (Hasan et al 2016). Other wildlife including wolves, seabreams, lynxes, wild boars, foxes, deers, bats, and rodents can also be potential reservoirs of ESBL-producing bacteria (Nhung et al 2015;Crist ovão et al 2017;Schaufler et al 2018;Wasyl et al 2018;Garcês et al 2019). In addition, flies near livestock have been shown to carry ESBL-producing bacteria (dos Santos Alves et al 2018).…”

Section: Wildlife As Potential Reservoirs Of Esblproducing Bacteriamentioning

confidence: 99%

Prevalence of extended-spectrum β-lactamases in the local farm environment and livestock: challenges to mitigate antimicrobial resistance

Lee

Mir

Park

et al. 2020

Critical Reviews in Microbiology

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The effectiveness of antibiotics has been challenged by the increasing frequency of antimicrobial resistance (AR), which has emerged as a major threat to global health. Despite the negative impact of AR on health, there are few effective strategies for reducing AR in food-producing animals. Of the antimicrobial resistant microorganisms (ARMs), extended-spectrum b-lactamases (ESBLs)-producing Enterobacteriaceae are an emerging global threat due to their increasing prevalence in livestock, even in animals raised without antibiotics. Many reviews are available for the positive selection of AR associated with antibiotic use in livestock, but less attention has been given to how other factors including soil, water, manure, wildlife, and farm workers, are associated with the emergence of ESBL-producing bacteria. Understanding of antibiotic resistance genes and bacteria transfer at the interfaces of livestock and other potential reservoirs will provide insights for the development of mitigation strategies for AR.

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First report on extended-spectrum beta-lactamase (ESBL) producing Escherichia coli from European free-tailed bats (Tadarida teniotis) in Portugal: A one-health approach of a hidden contamination problem

Cited by 18 publications

References 47 publications

Characterization of ESBL-Producing Enterobacteria from Fruit Bats in an Unprotected Area of Makokou, Gabon

Characterization of ESBL-Producing Enterobacteria from Fruit Bats in an Unprotected Area of Makokou, Gabon

Prevalence of Extended-Spectrum β-Lactamases in E. coli of Rats in the Region North East of Gabon

Prevalence of extended-spectrum β-lactamases in the local farm environment and livestock: challenges to mitigate antimicrobial resistance

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