Prevalence of extended-spectrum β-lactamases in the local farm environment and livestock: challenges to mitigate antimicrobial resistance

Lee, Shin-Young; Mir, Raies A.; Park, Si Hong; Kim, Donghyuk; Kim, Hae Yeong; Boughton, Raoul K.; Morris, J. Glenn; Jeong, Kwangcheol C.

doi:10.1080/1040841x.2020.1715339

Cited by 51 publications

(32 citation statements)

References 133 publications

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“…This finding could be attributed to the overuse or misuse of antimicrobials in animal husbandry [83] and is alarming since products derived from these animals are included in the daily human dietary [58]. Furthermore, MDR E. coli strains could potentially be transmitted from farmed animals to wildlife species or vice versa [84,85]. The magpie strain harbored resistance genes for all the tested antimicrobial classes, a fact that could be ascribed to antibiotic residues and ESBL-producing strains present in the environment due to human and livestock influence [86], as well as to the bird's scavenging behavior.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance Genes in ESBL-Producing Escherichia coli Isolates from Animals in Greece

et al. 2021

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The prevalence of multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Enterobacteriaceae is increasing worldwide. The present study aimed to provide an overview of the multidrug resistance phenotype and genotype of ESBL-producing Escherichia coli (E. coli) isolates of livestock and wild bird origin in Greece. Nineteen phenotypically confirmed ESBL-producing E. coli strains isolated from fecal samples of cattle (n = 7), pigs (n = 11) and a Eurasian magpie that presented resistance to at least one class of non β-lactam antibiotics, were selected and genotypically characterized. A DNA-microarray based assay was used, which allows the detection of various genes associated with antimicrobial resistance. All isolates harbored blaCTX-M-1/15, while blaTEM was co-detected in 13 of them. The AmpC gene blaMIR was additionally detected in one strain. Resistance genes were also reported for aminoglycosides in all 19 isolates, for quinolones in 6, for sulfonamides in 17, for trimethoprim in 14, and for macrolides in 8. The intI1 and/or tnpISEcp1 genes, associated with mobile genetic elements, were identified in all but two isolates. This report describes the first detection of multidrug resistance genes among ESBL-producing E. coli strains retrieved from feces of cattle, pigs, and a wild bird in Greece, underlining their dissemination in diverse ecosystems and emphasizing the need for a One-Health approach when addressing the issue of antimicrobial resistance.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance Genes in ESBL-Producing Escherichia coli Isolates from Animals in Greece

et al. 2021

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“…Notably, these genes were also lower in Li-f and No-f. Recently, E. coli carrying both mcr-1 and ESBL in the plasmid in swine in China have been reported (Malhotra-Kumar et al, 2016;Ji et al, 2019;Lee et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance in Swine Fecal Specimens Across Different Farm Management Systems

et al. 2020

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Antimicrobial use in agricultural animals is known to be associated with increases in antimicrobial resistance. Most prior studies have utilized culture and susceptibility testing of select organisms to document these phenomena. In this study we aimed to detect 66 antimicrobial resistance (AMR) genes for 10 antimicrobial agent classes directly in swine fecal samples using our previously developed antimicrobial resistance TaqMan array card (AMR-TAC) across three different swine farm management systems. This included 38 extensive antimicrobial use (both in treatment and feed), 30 limited antimicrobial use (treatment only), and 30 no antimicrobial use farms. The number of resistance genes detected in extensive antimicrobial use farms was higher than in limited and no antimicrobial use farms (28.2 genes ± 4.2 vs. 24.0 genes ± 4.1 and 22.8 genes ± 3.6, respectively, p < 0.05). A principal component analysis and hierarchical clustering of the AMR gene data showed the extensive use farm samples were disparate from the limited and no antimicrobial use farms. The prevalence of resistance genes in extensive use farms was significantly higher than the other farm categories for 18 resistance genes including bla SHV , bla CTX−M1 group, bla CTX−M9 group, bla VEB , bla CMY2−LAT, aac(6)-lb-cr, qnrB1, gyrA83L-E. coli, armA, rmtB, aac(3)-IIa, mphA, 23S rRNA 2075G-Campylobacter spp., mcr-1, catA1, floR, dfrA5-14, and dfrA17. These genotypic findings were supported by phenotypic susceptibility results on fecal E. coli isolates. To examine the timing of AMR gene abundance in swine farms, we also performed a longitudinal study in pigs. The results showed that AMR prevalence occurred both early, presumably from mothers, as well as after weaning, presumably from the environment. In summary, detection of AMR genes directly in fecal samples can be used to qualitatively and quantitatively monitor AMR in swine farms.

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“…These findings may suggest that IncN plasmids play a key role in β-lactamase gene dissemination in manure and soil bacteria. Beyond their insertion in mobilizable plasmids, ESBL genes are located in insertion sequences, like IS Ecp1 and IS CR1 , which facilitate their spread [ 81 ].…”

Section: Composition Of Manurementioning

confidence: 99%

Manure as a Potential Hotspot for Antibiotic Resistance Dissemination by Horizontal Gene Transfer Events

Lima

Domingues

Silva

2020

Veterinary Sciences

115

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The increasing demand for animal-derived foods has led to intensive and large-scale livestock production with the consequent formation of large amounts of manure. Livestock manure is widely used in agricultural practices as soil fertilizer worldwide. However, several antibiotic residues, antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria are frequently detected in manure and manure-amended soils. This review explores the role of manure in the persistence and dissemination of ARGs in the environment, analyzes the procedures used to decrease antimicrobial resistance in manure and the potential impact of manure application in public health. We highlight that manure shows unique features as a hotspot for antimicrobial gene dissemination by horizontal transfer events: richness in nutrients, a high abundance and diversity of bacteria populations and antibiotic residues that may exert a selective pressure on bacteria and trigger gene mobilization; reduction methodologies are able to reduce the concentrations of some, but not all, antimicrobials and microorganisms. Conjugation events are often seen in the manure environment, even after composting. Antibiotic resistance is considered a growing threat to human, animal and environmental health. Therefore, it is crucial to reduce the amount of antimicrobials and the load of antimicrobial resistant bacteria that end up in soil.

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Prevalence of extended-spectrum β-lactamases in the local farm environment and livestock: challenges to mitigate antimicrobial resistance

Cited by 51 publications

References 133 publications

Antimicrobial Resistance Genes in ESBL-Producing Escherichia coli Isolates from Animals in Greece

Antimicrobial Resistance Genes in ESBL-Producing Escherichia coli Isolates from Animals in Greece

Antimicrobial Resistance in Swine Fecal Specimens Across Different Farm Management Systems

Manure as a Potential Hotspot for Antibiotic Resistance Dissemination by Horizontal Gene Transfer Events

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