First report on classical and molecular cytogenetics of archerfish, Toxotes chatareus (Perciformes: Toxotidae)

Supiwong, Weerayuth; Jiwyam, Wirat; Sreeputhorn, K.; Maneechot, Nuntiya; Bertollo, Luiz Antônio Carlos; Cioffi, Marcelo de Bello; Getlekha, Nuntaporn; Tanomtong, Alongklod

doi:10.1007/s13237-017-0216-5

Cited by 9 publications

(13 citation statements)

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“…lucius (Cioffi et al 2015) but they differ from other Channa that they are distributed throughout the chromosome without high accommodations in the regions related with NOR positions. Moreover, the patterns are also different from T. chatareus (Supiwong et al 2017) and Asian swamp eel, M. albus (Supiwong et al 2019) and the wolf fish (Hoplias malabaricus) in which 12 different microsatellite repeats, including d(CA)15, d(GA)15, d(CAA)10, and d(CGG)10 repeats showed strong hybridization signals at subtelomeric and heterochromatic regions of several autosomes, with a varied amount of signal on the sex chromosomes (Cioffi et al 2011). From the previous studies, it is believed that microsatellites have specific zones as heterochromatin (telomeres, centromeres, and in the sex chromosomes) of fish genomes (Cioffi and Bertollo 2012).…”

Section: Discussionmentioning

confidence: 83%

“…Comparisons with other families revealed that the d(CA)15 microsatellite repeats mapping are similar to the nine species of the Bagridae family including Hemibagrus filamentus, H. spilopterus, H. wyckii, H. wyckioides, Mystus atrifasciatus, M. multiradiatus, M. mysticetus, M. bocourti, andPseudomystus siamensis (Supiwong et al 2013a, 2014b), Clarias batrachus and C. macrocephalus (Maneechot et al 2016), Toxotes chatareus (Supiwong et al 2017). This pattern can be found in non-Thai fish species.…”

Section: Discussionmentioning

confidence: 88%

“…The post hybridization wash was carried out with 1×SSC for 5 min at 65°C. A final wash was performed at room temperature in 4×SSCT for 5 min (Supiwong et al 2017(Supiwong et al 2019. Finally, the slides were counterstained with DAPI and mounted in an antifade solution (Vectashield from Vector laboratories), and analyzed in an epifluorescence microscope Olympus BX50 (Olympus Corporation, Ishikawa, Japan).…”

Section: Fish Techniquementioning

confidence: 99%

“…However, this kind of study is scarcely applied in Thai freshwater fish. Exceptions are studies in Bagrid catfishes (Supiwong et al 2013(Supiwong et al , 2014a, Channids (Cioffi et al 2015), three Clarias species (Maneechot et al 2016), Toxotes chatareus (Hamilton, 1822) (Supiwong et al 2017), two cyprinid fishes (Saenjundaeng et al 2018), Asian swamp eel, Monopterus albus (Zuiew, 1793) (Supiwong et al 2019) and silulid catfishes (Ditcharoen et al 2019). The present study is the first report on chromosomal characteristics in P. cochinchinensis using conventional staining, Ag-NOR banding, and FISH technique, being the basis for further study by advanced genetics and for future comparative approaches concerning systematics and evolutionary relationships of buffer-fishes.…”

Section: Introductionmentioning

confidence: 99%

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First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866)

et al. 2020

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Abstract. Pissaparn M, Phimphan S, Chaiyasan P, Tanoamtong A, Liehr T, Suwannapoom C, Reungsing M, Supiwong W. 2020. First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866). Biodiversitas 21: 4309-4316. Here first analysis of chromosomes and nucleolar organizer region (NOR) pattern in pufferfish Pao cochinchinensis (Steindachner, 1866) was undertaken. Chromosomal preparations were obtained from kidney of P. cochinchinensis from Chi River basin in Thailand. Chromosomal characteristics were analyzed by Giemsa staining, Ag-NOR banding as well as fluorescence in situ hybridization (FISH) using microsatellites d(CA)15 and d(CGG)10 probes. P. cochinchinensis had 2n = 40 with the fundamental number (NF) 74, both in male and female. The karyotype exhibited 12 metacentric (m), 10 submetacentric (sm), 12 acrocentric (a) and 6 telocentric (t) chromosomes. No differentiated heteromorphic sex chromosomes were observed. NORs were located on short arms adjacent to telomere of the metacentric chromosome pair 4, which coincide with signals of d(CGG)10 probe. FISH with d(CGG)10 sequences were also displayed at the telomeres of most other chromosomes, whereas d(CA)15 repeats highly accumulated throughout almost all entire chromosomes except for centromeric regions. The results of conventional Giemsa staining presented the differentiation even the same genus. The localization of NORs on one pair of chromosomes only is a common characteristic found in many fish groups as well as other vertebrates. Mapping of two distinct microsatellites demonstrated the remarkable chromosomal diversification that characterizes evolution in the genus Pao. Both, conventional and molecular cytogenetics are excellent tools to study, and better understand chromosomal evolution, as well as to uncover biodiversity among fishes.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 88%

Section: Fish Techniquementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866)

et al. 2020

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“…Among Carangaria several families, like Centropomidae, Carangidae, Sphyraenidae, Coryphaenidae, Toxotidae, and Rachycentridae, have karyotypes mainly composed of 2n=48 chromosomes (Accioly et al, 2012;Costa et al, 2015;Soares et al, 2017;Supiwong et al, 2017;Borges et al, 2019;Motta-Neto et al, 2019), while Pleuronectiformes (mainly Pleuronectodei), have chromosome numbers ranging from 2n=26 to 2n=48 (Azevedo et al, 2007;Arai, 2011). Most of the karyotype variations are due to pericentric inversions and Robertsonian translocations, which promote an increase of the NF (number of chromosome arms) and reduction of the diploid number, as well as increasing polymorphisms in a species, providing variation for natural selection and can even lead to speciation.…”

Section: Discussionmentioning

confidence: 99%

Research Article The unusual high number of chromosomes signals rare multiple fission events in the Polynemidae (Carangaria, Teleostei)

Molina¹,

Cioffi²,

Soares³

et al. 2021

Genet. Mol. Res.

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Polynemidae, commonly known as threadfins, are important fishing and aquaculture species. This is a peculiar fish group that is morphologically characterized by long tactile filaments of the pectoral fins. Although inserted in the Percomorpha, its relationships with other groups of this clade are uncertain. Cytogenetic investigations have helped clarify various evolutionary aspects of marine fish groups, including stock delimitation, speciation, taxonomy, hybridization, phylogenetic relationship, sex chromosomes, and genome changes. However, the chromosomal characteristics of threadfins are still unknown. We investigated the chromosomal features of two cryptic species, Polydactylus virginicus and P. oligodon found in Northeast Brazilian coastal waters (Tropical Southwestern Atlantic region). Cytogenetic data were obtained by conventional staining (Giemsa and fluorochrome staining, C-banding and Ag-NORs techniques) and molecular cytogenetic analyses through fluorescent in situ hybridization using rDNA probes (18S and 5S rDNA). The two species have similar karyotypes at the macro and microstructural levels. An unexpected high diploid number was found, with 2n=58 acrocentric chromosomes, including four ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 20 (1): gmr18701 W.F. Molina et al. 2 microchromosome pairs. The expanded karyotypes are a synapomorphy for these species, likely resulting from sharing at least five chromosome fissions from a basal karyotype with 2n=48. Besides the macrostructural karyotype similarities, the Ag-NOR/GCrich/18S rDNA (chromosome 19) and 5S rDNA (chromosome 20) loci have an identical organization in apparently homeolog chromosomes of these species, suggesting the conservation of large syntenic chromosomal regions. We conclude that although chromosome fissions are very rare events among other Carangaria fish, they played an important disruptive role in the evolution of some species of Polynemidae.

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Comparative cytogenomic analysis of Cardinal fishes (Perciformes, Apogonidae) from Thailand

Kasiroek

Phimphan

Pinthong

et al. 2021

Nucleus

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First report on classical and molecular cytogenetics of archerfish, Toxotes chatareus (Perciformes: Toxotidae)

Cited by 9 publications

References 49 publications

First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866)

First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866)

Research Article The unusual high number of chromosomes signals rare multiple fission events in the Polynemidae (Carangaria, Teleostei)

Comparative cytogenomic analysis of Cardinal fishes (Perciformes, Apogonidae) from Thailand

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