The first chromosome analysis and nucleolar organizer region (NOR) pattern of the humpback cardinalfish (Fibramia lateralis) were studied. Samples from 10 male and 10 female fish were collected from the Andaman Sea and Gulf of Thailand. Mitotic chromosome preparations were prepared directly from kidney tissues. Conventional and Ag-NOR staining techniques were applied to stain the chromosomes. The results showed that the diploid chromosome number of F. lateralis was 2n=46, and the fundamental numbers (NF) were 54 in both sexes. The karyotype consisted of 8 large acrocentric, 12 large telocentric, 24 medium telocentric and 2 small telocentric chromosomes. Moreover, the results indicated that the region adjacent to the telomere of the short arm of the second acrocentric chromosome pair showed clearly observable nucleolar organizer regions (NORs). Strange size chromosomes related to sex were not observed. The karyotype formula for F. lateralis is as follows: a t t t 8 12 24 2 2 (diploid) 46=L +L +M +S n
This is the first nucleolar organizer region (NOR) polymorphism and chromosome analysis of Banggai cardinalfish (Pterapogon kauderni Koumans, 1933). Kidney cell samples were taken from 10 male and 10 female fish. Mitotic chromosome preparations were prepared directly from kidney cells. Conventional and Ag-NOR banding techniques were applied to stain the chromosomes. The results showed that the diploid chromosome number of P. kauderni was 2n=46, and the fundamental number (NF) was 92 in both males and females. The types of chromosomes were 6 large acrocentric, 4 medium metacentric, 14 medium submetacentric, and 22 medium acrocentric chromosomes. The results indicated that the short arm subtelomeric of the acrocentric chromosome pair 13 showed clearly observable NORs. This finding exhibited that three NOR polymorphism patterns were found: 1) homomorphic which shows an equal size of both chromosome pair 13 (13a13a), 2) heteromorphic that displays different sizes of NORs of chromosome pair 13 (13a13c) and 3) heteromorphic which is found in only one homologous chromosome pair 13 (13a13b). There was no observation of strange size chromosomes related to sex. The karyotype formula for P. kauderni was: a m sm a 6 4 14 22 2 (diploid) 46 = L + M + M + M n
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