First Report of Zucchini tigre mosaic virus Infecting Several Cucurbit Plants in China

Virome Profiling, New Virus Identification and the Prevalence and Distribution of Viruses Infecting Chieh-Qua (Benincasa hispida Cogn. var. chieh-qua How) in China

“…Diverse virus-like symptoms, including mosaic, interveinal chlorosis, mottling and leaf deformation, are frequently observed in chieh-qua; however, there has been limited research on sporadic chieh-qua samples and only four viruses have been reported: zucchini tigre mosaic virus (ZTMV, genus Potyvirus ), zucchini yellow mosaic virus (ZYMV, genus Potyvirus ), papaya ringspot virus (PRSV, genus Potyvirus ) and cucumber mosaic virus (CMV, genus Cucumovirus ) [ 6 , 7 , 8 ]. ZYMV, PRSV and CMV have been frequently identified in cucurbit vegetables in China [ 9 ], whereas ZTMV has only been discovered in China in recent years, with a potential risk to infect more cucurbit crops in the field [ 9 , 10 ]. Recently, Nong [ 11 ] investigated both wax gourd and chieh-qua plants in Guangdong Province, China, and they discovered the above four viruses as well as six other viruses: cucurbit aphid-borne yellows virus (CABYV), melon aphid-borne yellows virus (MABYV), squash leaf curl China virus (SLCCNV), cucumber green mottle mosaic virus (CGMMV), watermelon silver mottle virus (WSMoV) and tobacco stripe virus (TSV).…”

Complete genome sequence and construction of an infectious full-length cDNA clone of zucchini tigre mosaic virus isolate infecting snake gourd

“…However, biological, serological and molecular biology studies suggest that it should be a distinct species within the genus Potyvirus [3]. To date, ZTMV has been reported across three continents, including Asia, Europe, and the Americas, as well as in some Caribbean and Indian Ocean islands [3][4][5][6][7][8][9]. The rst Chinese isolate of ZTMV was reported in 2014, and has since been recognized as a predominant pathogen impacting cucurbit production due to Author contributions Cuiji and Chengrong Nie conceived and designed the experiments.…”

Data_Sheet_1.docx

“…Zucchini tigre mosaic potyvirus has been detected in cucurbit crops grown in Asia, Europe, and America, as well as some Caribbean and Indian Ocean islands (Romay et al, 2014;Xiao et al, 2016;Desbiez et al, 2017;Abdalla and Ali, 2018;Wang et al, 2019); however, to date only seven complete genome sequences of ZTMV are available in GenBank (Figure 3). In China, ZTMV was first reported in pumpkin, zucchini, and cucumber of Yunnan province, located in southwestern China near Myanmar.…”

“…Therefore, based on molecular and biological evidence, ZTMV has been proposed as a distinct potyvirus in the PRSV cluster (Quiot-Douine et al, 1986;Romay et al, 2014). ZTMV has been reported in three continents (Asia, Europe, and America) and in some Caribbean and Indian Ocean islands (Romay et al, 2014;Xiao et al, 2016;Desbiez et al, 2017;Abdalla and Ali, 2018;Wang et al, 2019). Based on phylogenetic analysis of the complete CP nt sequence, ZTMV isolates are classified into three subgroups: Asian, Indian Ocean, and American, related to their geographical origins (Romay et al, 2014).…”

“…Based on phylogenetic analysis of the complete CP nt sequence, ZTMV isolates are classified into three subgroups: Asian, Indian Ocean, and American, related to their geographical origins (Romay et al, 2014). In 2014, the first Chinese isolate of ZTMV was reported in cucurbit plants from Yunnan province of China, which was identified as a major pathogen affecting cucurbit production in Yunnan province due to its wide distribution (Xiao et al, 2016). Moreover, two ZTMV isolates infecting wax gourd (Benincasa hispida) under accession number MN267689 and bitter melon (Momordica charantia) under accession number LC371337 have been detected in Guangdong and Taiwan provinces, respectively.…”