First Report of Xanthomonas euvesicatoria on Tomato in Bulgaria

Kizheva, Yoana; Vancheva-Ebben, Taca; Hristova, Petya; Bogatzevska, Nevena; Moncheva, Penka

doi:10.7546/crabs.2020.01.18

Cited by 4 publications

(8 citation statements)

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“…They differ in their year and location of isolation, pathotype and races (Table 1). All strains were previously identified and characterized (Kizheva et al 2013(Kizheva et al , 2020Bogatzevska and Sotirova, 2002). The following type cultures were used as controls in the host range determination: Xanthomonas euvesicatoria NBIMCC 8731, Xanthomonas perforans NBIMCC 8729, Xanthomonas vesicatoria NBIMCC 2427, Xanthomonas gardneri NBIMCC 8730 and Pseudomonas syringae pv.…”

Section: Bacterial Cultures and Cultivation Conditionsmentioning

confidence: 99%

“…The major bacterial spot causative agents on tomato plants in Bulgaria are X. vesicatora followed by X. gardneri and on pepper plants -X. euvesicatoria (Kizheva et al, 2011(Kizheva et al, , 2013Vancheva et al, 2018). The first report of X. euvesicatoria isolated from infected tomato plants in Bulgaria was made in 2020 (Kizheva et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Broad host range bacteriophages found in rhizosphere soil of a healthy tomato plant in Bulgaria

Kizheva

Eftimova

Rangelov

et al. 2021

Heliyon

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Section: Bacterial Cultures and Cultivation Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Broad host range bacteriophages found in rhizosphere soil of a healthy tomato plant in Bulgaria

Kizheva

Eftimova

Rangelov

et al. 2021

Heliyon

Self Cite

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“…To date, three species ( X. vesicatoria, X. euvesicatoria , and X. gardneri ) that infect tomato and two species ( X. vesicatoria and X. euvesicatoria ) that infect pepper have been described in Bulgaria. The natural population of pathogens is heterogeneous by symptoms, species, phenotypic and genotypic characteristics, pathotype, and races [ 4 , 5 , 6 , 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…The control of the disease and the battle against bacterial spot agents are extremely difficult due to several factors, such as the dynamic changes in the species and race composition; the cyclicity of their natural populations; their symptomatic and symptomless development in hosts and non-hosts; their relationships with the normal microbiota of host plants; their ability to be stored in a hypobiotic state in seeds and to multiply and reproduce again; and the limited effectiveness of the most commonly used chemical control agents, such as copper-based pesticides, due to the emergence and spread of resistant strains [ 1 , 2 , 4 , 5 , 6 , 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and Genotypic Characterization of Newly Isolated Xanthomonas euvesicatoria-Specific Bacteriophages and Evaluation of Their Biocontrol Potential

Kizheva

Urshev²,

Dimitrova

et al. 2023

Plants

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Bacteriophages have greatly engaged the attention of scientists worldwide due to the continuously increasing resistance of phytopathogenic bacteria to commercially used chemical pesticides. However, the knowledge regarding phages is still very insufficient and must be continuously expanded. This paper presents the results of the isolation, characterization, and evaluation of the potential of 11 phage isolates as natural predators of a severe phytopathogenic bacterium—Xanthomonas euvesicatoria. Phages were isolated from the rhizosphere of tomato plants with symptoms of bacterial spot. The plaque morphology of all isolates was determined on a X. euvesicatoria lawn via a plaque assay. Three of the isolates were attributed to the family Myoviridae based on TEM micrographs. All phages showed good long-term viability when stored at 4°C and -20°C. Three of the phage isolates possessed high stability at very low pH values. Fifty-five-day persistence in a soil sample without the presence of the specific host and a lack of lytic activity on beneficial rhizosphere bacteria were found for the phage isolate BsXeu269p/3. The complete genome of the same isolate was sequenced and analyzed, and, for the first time in this paper, we report a circular representation of a linear but circularly permuted phage genome among known X. euvesicatoria phage genomes.

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“…X. euvesicatoria was identified as the dominant species of bacterial spot of pepper plants in Bulgaria and North Macedonia [ 49 , 50 ]. After 2014, the species was also reported as a bacterial spot agent of tomato in Bulgaria [ 51 ]. Analyses using restriction fragment length polymorphisms (RFLP), randomly amplified polymorphic DNA (RAPD) markers and rep-PCR revealed substantial genetic diversity among the Bulgarian and North Macedonian X. euvesicatoria strains [ 52 , 53 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology of Xanthomonas euvesicatoria Strains from the Balkan Peninsula Revealed by a New Multiple-Locus Variable-Number Tandem-Repeat Analysis Scheme

et al. 2021

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Bacterial spot of pepper and tomato is caused by at least three species of Xanthomonas, among them two pathovars of Xanthomonas euvesicatoria, which are responsible for significant yield losses on all continents. In order to trace back the spread of bacterial spot pathogens within and among countries, we developed the first multilocus variable number of tandem repeat analyses (MLVA) scheme for pepper- and tomato-pathogenic strains of X. euvesicatoria. In this work, we assessed the repeat numbers by DNA sequencing of 16 tandem repeat loci and applied this new tool to analyse a representative set of 88 X. euvesicatoria pepper strains from Bulgaria and North Macedonia. The MLVA-16 scheme resulted in a Hunter–Gaston Discriminatory Index (HGDI) score of 0.944 and allowed to resolve 36 MLVA haplotypes (MTs), thus demonstrating its suitability for high-resolution molecular typing. Strains from the different regions of Bulgaria and North Macedonia were found to be widespread in genetically distant clonal complexes or singletons. Sequence types of the variable number of tandem repeats (VNTR) amplicons revealed cases of size homoplasy and suggested the coexistence of different populations and different introduction events. The large geographical distribution of MTs and the existence of epidemiologically closely related strains in different regions and countries suggest long dispersal of strains on pepper in this area.

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First Report of Xanthomonas euvesicatoria on Tomato in Bulgaria

Cited by 4 publications

References 0 publications

Broad host range bacteriophages found in rhizosphere soil of a healthy tomato plant in Bulgaria

Broad host range bacteriophages found in rhizosphere soil of a healthy tomato plant in Bulgaria

Phenotypic and Genotypic Characterization of Newly Isolated Xanthomonas euvesicatoria-Specific Bacteriophages and Evaluation of Their Biocontrol Potential

Molecular Epidemiology of Xanthomonas euvesicatoria Strains from the Balkan Peninsula Revealed by a New Multiple-Locus Variable-Number Tandem-Repeat Analysis Scheme

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