Broad host range bacteriophages found in rhizosphere soil of a healthy tomato plant in Bulgaria

Kizheva, Yoana; Eftimova, Melani; Rangelov, Radoslav; Micheva, Neli; Urshev, Zoltan; Rasheva, Iliyana Kirilova; Hristova, Petya

doi:10.1016/j.heliyon.2021.e07084

Cited by 10 publications

(13 citation statements)

References 54 publications

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“…The present study focused on the isolation, characterization (phenotypic and genotypic), and evaluation of the potential of newly isolated phages as biocontrol agents targeting the causative agent of spot scab on both pepper and tomato in Bulgaria— X. euvesicatoria . This study was a continuation of our previous work [ 29 ].…”

Section: Discussionsupporting

confidence: 53%

“…In our case, the rhizosphere of the infected tomato plants could also be used as a source for the isolation of XESP. Elsewhere, the rhizosphere soil of healthy tomato plants was reported by our team as a source of isolation for phages infecting X. vesicatoria and X. gardneri [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

“…There have only been a few reports on the isolation, characterization, and biocontrol potential evaluation of phages effective against bacterial-spot-causing xanthomonads: phages infecting Xanthomonas campestris pv. vesicatoria [ 22 , 23 , 24 ]; phages infecting X. euvesicatoria ( Myoviridae phages isolated in Serbia) [ 25 , 26 ]; phages infecting X. vesicatoria (an Inoviridae phage [ 27 ], a Myoviridae phage [ 28 ], and one presumptive Podoviridae phage) [ 29 ]; phages infecting X. perforans (unidentified phages) [ 30 ]; and phages infecting X. gardneri (the only report is for one broad-host-range (polyvalent) phage) [ 29 ]. Moreover, according to GenBank database, there is too scarce data on the genome organization of Xanthomonas phages, with only 134 fully sequenced genomes compared to 133 for Escherichia coli phages, 543 for Acinetobacter phages, 231 for Erwinia phages, etc.…”

Section: Introductionmentioning

confidence: 99%

“…For example, the tolerance of the X. euvesicatoria phage KΦ1 to different pH values, storage temperatures, and UV radiation in vitro has been reported [ 26 ]. The same characteristics have been studied for broad-host-range bacteriophages effective against X. euvesicatoria , X. vesicatoria , and X. gardneri [ 29 ]. Perhaps among the less studied characteristics of phages are their survival in the soil after application and their impact on the rhizosphere microbiome of the plant, excluding a few reports [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

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Phenotypic and Genotypic Characterization of Newly Isolated Xanthomonas euvesicatoria-Specific Bacteriophages and Evaluation of Their Biocontrol Potential

Kizheva

Urshev²,

Dimitrova

et al. 2023

Plants

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Bacteriophages have greatly engaged the attention of scientists worldwide due to the continuously increasing resistance of phytopathogenic bacteria to commercially used chemical pesticides. However, the knowledge regarding phages is still very insufficient and must be continuously expanded. This paper presents the results of the isolation, characterization, and evaluation of the potential of 11 phage isolates as natural predators of a severe phytopathogenic bacterium—Xanthomonas euvesicatoria. Phages were isolated from the rhizosphere of tomato plants with symptoms of bacterial spot. The plaque morphology of all isolates was determined on a X. euvesicatoria lawn via a plaque assay. Three of the isolates were attributed to the family Myoviridae based on TEM micrographs. All phages showed good long-term viability when stored at 4°C and -20°C. Three of the phage isolates possessed high stability at very low pH values. Fifty-five-day persistence in a soil sample without the presence of the specific host and a lack of lytic activity on beneficial rhizosphere bacteria were found for the phage isolate BsXeu269p/3. The complete genome of the same isolate was sequenced and analyzed, and, for the first time in this paper, we report a circular representation of a linear but circularly permuted phage genome among known X. euvesicatoria phage genomes.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Phenotypic and Genotypic Characterization of Newly Isolated Xanthomonas euvesicatoria-Specific Bacteriophages and Evaluation of Their Biocontrol Potential

Kizheva

Urshev²,

Dimitrova

et al. 2023

Plants

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“…At first, we attempted to isolate phages from soil collected at healthy groves. Although the isolation of Xanthomonas phages from soil collected in healthy orchards has been reported [ 41 ], our attempts with this kind of sample were not successful. We also tried to isolate phages by mixing the soil samples with NB medium and filtering the samples before inoculating them with Xac 306, but this approach was also unproductive.…”

Section: Discussionmentioning

confidence: 93%

Isolation and characterization of vB_XciM_LucasX, a new jumbo phage that infects Xanthomonas citri and Xanthomonas fuscans

et al. 2022

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Citrus canker is one of the main bacterial diseases that affect citrus crops and is caused by Xanthomonas citri which affects all citrus species worldwide. New strategies to control citrus canker are necessary and the use of bacteriophages as biocontrol agent could be an alternative. Phages that infect Xanthomonas species have been studied, such as XacN1, a myovirus that infects X. citri. Here we report the isolation and characterization of a new jumbo phage, vb_XciM_LucasX, which infects X. citri and X. fuscans. Transmission electron microscopy allowed classification of LucasX in the Myoviridae family, which was corroborated by its genomic sequencing, annotation, and proteome clustering. LucasX has a 305,651 bp-long dsDNA genome. ORF prediction and annotation revealed 157 genes encoding putative structural proteins such as capsid and tail related proteins and phage assembly associated proteins, however, for most of the structural proteins it was not possible assign specific functions. Its genome encodes several proteins related to DNA replication and nucleotide metabolism, five putative RNA polymerases, at least one homing endonuclease mobile element, a terminase large subunit (TerL), an endolysin and many proteins classified as beneficial to the host. Proteome clustering and phylogeny analyses showed that LucasX is a new jumbo phage having as its closest neighbor the Xanthomonas jumbo phage Xoo-sp14. LucasX presented a burst size of 40 PFU/infected cell of X. citri 306, was completely inactivated at temperatures above 50°C, presented survival lower than 25% after 80 s of exposition to artificial UV light and had practically no tolerance to concentrations above 2.5 g/L NaCl or 40% ethanol. LucasX presented optimum pH at 7 and a broad range of Xanthomonas hosts, infecting twenty-one of the twenty-three strains tested. Finally, the LucasX yield was dependent on the host strain utilized, resulting one order of magnitude higher in X. fuscans C 752 than in X. citri 306, which points out to the possibility of phage yield improvement, an usual challenge for biocontrol purposes.

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Importance of Microbial Inoculants in Maintaining Plant Growth and Productivity

Bhutani,

Mehra,

Mishra

et al. 2024

Microbial Inoculants

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Broad host range bacteriophages found in rhizosphere soil of a healthy tomato plant in Bulgaria

Cited by 10 publications

References 54 publications

Phenotypic and Genotypic Characterization of Newly Isolated Xanthomonas euvesicatoria-Specific Bacteriophages and Evaluation of Their Biocontrol Potential

Phenotypic and Genotypic Characterization of Newly Isolated Xanthomonas euvesicatoria-Specific Bacteriophages and Evaluation of Their Biocontrol Potential

Isolation and characterization of vB_XciM_LucasX, a new jumbo phage that infects Xanthomonas citri and Xanthomonas fuscans

Importance of Microbial Inoculants in Maintaining Plant Growth and Productivity

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