First Report of Prevalence of CTX-M-15-Producing <i>Escherichia coli</i> O25b/ST131 from Iran

Namaei, Mohammad Hasan; Yousefi, Masoud; Ziaee, Masoud; Salehabadi, Alireza; Ghannadkafi, Malaknaz; Amini, Elham; Askari, Parvin

doi:10.1089/mdr.2016.0272

Cited by 27 publications

(29 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In isolates belonging to CGA, the TMU7 isolate exerted a higher (68.61%) apoptotic activity (p = 0.04). The that 38 out of the 154 B2 group isolates (24.7%) belonged to the ST131 clone and had significantly higher antibiotic resistance than non-ST131 strains (Namaei et al, 2017).…”

Section: Neutrophil Apoptosismentioning

confidence: 93%

Characterization of uropathogenic E. coli O25b‐B2‐ST131, O15:K52:H1, and CGA: Neutrophils apoptosis, serum bactericidal assay, biofilm formation, and virulence typing

Mostafavi

Peerayeh

Mobarez

et al. 2019

Journal Cellular Physiology

View full text Add to dashboard Cite

Pathogenic and drug‐resistant strains of Escherichia coli (E. coli) O25b‐B2‐ST131, O15:H1‐D‐ST393, and CGA (clonal group A) clonal groups have spread worldwide. This study aimed at determining E. coli epidemic clonal groups, their virulence factors, biofilm formation, neutrophils apoptosis, and antimicrobial resistance pattern of uropathogenic E. coli. A total of 95 CTX‐M‐1‐producing E. coli clinical isolates were enrolled. E. coli O25b‐B2‐ST131, CGA, and O15:K52:H1 were identified by serotyping and phylogrouping and allele‐specific polymerase chain reaction‐based assay. Antibiotic susceptibility, biofilm formation, hemolysis, and human serum bactericidal assay were performed. Neutrophil apoptosis was assayed by flow cytometry. Nine E. coli clonal groups including six O25b‐B2‐ST131 strains, two CGA, and one O15:K52:H1‐D‐ST393 strains were detected. One O25b‐B2‐ST131 isolate was a strong biofilm‐producer. Three ST131 isolates had type I fimbriae. Furthermore, all the CGA and O15:K52:H1 and three of ST131 isolates harbored the P fimbriae. The virulence genes ompT, fimH, and traT were detected among all the clonal groups. The apoptosis was induced by O25b‐B2‐ST131, CGA, and O15:K52:H1 E. coli. There was no significant difference regarding apoptosis induction among clonal groups. Furthermore, the presence of the cdt, usp, and vat genes was significantly associated with the apoptosis of neutrophils by O25b‐B2‐ST131, CGA, and O15:K52:H1‐D‐ST393 clonal groups.

show abstract

Section: Neutrophil Apoptosismentioning

confidence: 93%

Characterization of uropathogenic E. coli O25b‐B2‐ST131, O15:K52:H1, and CGA: Neutrophils apoptosis, serum bactericidal assay, biofilm formation, and virulence typing

Mostafavi

Peerayeh

Mobarez

et al. 2019

Journal Cellular Physiology

View full text Add to dashboard Cite

show abstract

“…(continued on next page) Source: ND -Not described isolate source. The virulent and multi-resistant CTX-M-15-producing E. coli O25b-ST131 clone is certainly one of the most well adapted circulant clones among E-ESBL, which is responsible for outbreaks and deaths around the world and is not related only to infectious processes, but is also reported in human intestinal colonization (elderly, adults and children) and animals (terrestrial and aquatic) and environmental contamination (Badran et al, 2016;Brahmi et al, 2015;Dolejska et al, 2011a;Ewers et al, 2010;Gonçalves et al, 2016;Namaei et al, 2017;Naseer et al, 2007;Olesen et al, 2013;Oteo et al, 2009;Owens et al, 2011;Zhong et al, 2015).…”

Section: Ctx-m-1mentioning

confidence: 99%

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

Palmeira

Ferreira

2020

Heliyon

View full text Add to dashboard Cite

Food producing animal is a global challenge in terms of antimicrobial resistance spread. Extended-spectrum betalactamase (ESBL)-producing Enterobacteriaceae are relevant opportunistic pathogens that may spread in many ecological niches of the One Health approach as human, animal and environment due to intestinal selection of antimicrobial resistant commensals in food production animals. Cattle production is a relevant ecological niche for selection of commensal bacteria with antimicrobial resistance from microbiota. Enterobacteriaceae show importance in terms of circulation of resistant-bacteria and antimicrobial resistance genes via food chain creating a resistance reservoir, setting up a threat for colonization of humans and consequent health risk. ESBL-producing Enterobacteriaceae are a threat in terms of human health responsible for life threatening outbreaks and silent enteric colonization of community populations namely the elder population. Food associated colonization is a risk difficult to handle and control. In a time of globalization of food trading, population intestinal colonization is a mirror of food production and in that sense this work aims to make a picture of ESBL-producing Enterobacteriaceae in animal production for food over the world in order to make some light in this reality of selection of resistant threats in food producing animal.

show abstract

“…Escherichia coli ( E. coli ) is one of the most important causes of UTI, whether it is hospital-acquired or community-acquired [ 2 ]. The emergence of E. coli sequence type 131 (ST131) as a multidrug-resistant (MDR) and virulent pathogen represents a major challenge to public health globally [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of E. coli ST131 among Uropathogenic E. coli Isolates from Iraqi Patients in Wasit Province, Iraq

Al-Guranie

Al-Mayahie

2020

International Journal of Microbiology

View full text Add to dashboard Cite

The emergence of Escherichia coli sequence type 131 (E. coli ST131) clone represents a major challenge to public health globally, since this clone is reported as highly virulent and multidrug-resistant, thus making it successfully disseminated worldwide. In Iraq, there is no previous study dealing with this important clone, so this project was suggested to investigate its presence within uropathogenic E. coli (UPEC) from Iraqi patients in Wasit Province. A total of 112 UPEC isolates from cases of acute urinary tract infection (UTI) were analysed for phylogenetic groups by quadruplex PCR; then, these isolates were investigated for E. coli ST131 clone by both conventional and real-time PCR procedures. The antibiotic susceptibility test was performed by the disk diffusion method. The results revealed that, out of 112 UPEC isolates, 38 (33.9%) belonged to phylogroup B2. For conventional PCR, 92.1% (35/38) of B2 E. coli isolates were positive for E. coli ST131, of which 34 were O25b-ST131 strain and 1 was O16-ST131 strain. However, serogroups O25b and O16 represented 17.1% and 2.8%, respectively. By RT-PCR assay, 15.1% (17/112) and 44.7% (17/38) of total and B2 E. coli isolates were confirmed as being E. coli ST131, respectively. The highest resistance rates of E. coli ST131 isolates were against the β-lactams, while low resistance rates were against amikacin, nitrofurantoin, and gentamicin. Fortunately, all isolates were susceptible to carbapenems. Moreover, 52.9% (9 out of 17) of E. coli ST131 isolates were MDR. In conclusion, the presence of E. coli ST131 among UPEC isolates from Iraqi patients is confirmed with high resistance to most antimicrobials included in this study.

show abstract

First Report of Prevalence of CTX-M-15-Producing Escherichia coli O25b/ST131 from Iran

Cited by 27 publications

References 24 publications

Characterization of uropathogenic E. coli O25b‐B2‐ST131, O15:K52:H1, and CGA: Neutrophils apoptosis, serum bactericidal assay, biofilm formation, and virulence typing

Characterization of uropathogenic E. coli O25b‐B2‐ST131, O15:K52:H1, and CGA: Neutrophils apoptosis, serum bactericidal assay, biofilm formation, and virulence typing

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

Prevalence of E. coli ST131 among Uropathogenic E. coli Isolates from Iraqi Patients in Wasit Province, Iraq

Contact Info

Product

Resources

About