First Report of Pepper Mild Mottle Virus in <i>Paris polyphylla</i> var. <i>yunnanensis</i> in China

Geyi, Wen; Yang, Ling; Anane, Rex Frimpong; Chen, Z. L.; Yang, Ying; Chen, L.; Sun, Y. X.; Zhao, Mingfu

doi:10.1094/pdis-03-19-0445-pdn

Cited by 21 publications

(16 citation statements)

References 4 publications

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“…Reports indicate that Paris mosaic necrosis virus (PMNV), Paris polyphylla virus X (PPVX), Pepper mild mottle virus (PMMoV), Cowpea aphid borne mosaic virus (CABMV), and Paris virus 1 (ParV1) infect and cause viral diseases in Paris polyphylla var. yunnanensis, leading to signi cant losses in plant yield [1][2][3][4][5][6] .…”

Section: Introductionmentioning

confidence: 99%

Characterization of a novel Tombusviridae species isolated from Paris polyphylla var. yunnanensis

et al. 2021

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A novel virus, Paris virus 2 (ParV2), was isolated from diseased Paris polyphylla var. yunnanensis, and its complete genome sequence was determined and analyzed. ParV2 is a positive-sense single-stranded RNA (+ssRNA) virus with a genome size of 4118 nucleotides. The ParV2 genome contains six putative open reading frames (ORFs) that encode proteins with predicted molecular weights of 40.14, 100.26, 7.31, 7.85, 26.09, and 8.77 kDa. The rst ORF (ORF1) of ParV2 encodes a putative protein of 40.14 kDa (p40, nt: 20-1096), whiles the second ORF (ORF2, 888 aa) containing the GDD motif encodes the highly conserved RNA-dependent RNA polymerase protein (RdRP, nt:20-2683, p100, 100.26 kDa) of viruses in the family Tombusviridae. Multiple sequence alignments analysis showed that the complete genome sequence of ParV2 shares 31.7-55.5% nucleotide sequence identities with viruses in the family Tombusviridae. Ginger chlorotic eck-associated tombusvirus (GCFaV-1,Accession No.QKE30557) had the highest sequence identity (55.5%) with ParV2, and also shares 59.2% RdRp and 34.9% CP amino acid sequence identity with GCFaV-1.Sequence comparisons and phylogenetic analysis of RdRp suggested that ParV2 is a novel member of the family Tombusviridae, and its closest known relative is GCFaV-1.

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Section: Introductionmentioning

confidence: 99%

Characterization of a novel Tombusviridae species isolated from Paris polyphylla var. yunnanensis

et al. 2021

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“…At present, four viruses have been reported to infect P. polyphylla var. yunnanensis, and these include the putative potexvirus Paris polyphylla virus X, the two potyviruses (Paris mosaic necrosis virus and Paris virus 1) and the tobamovirus Pepper mild mottle virus [5][6][7][8]. In this study, the complete genome sequence of a novel virus was identi ed in total RNAs extracted from diseased leaves of P. polyphylla var.…”

Section: Introductionmentioning

confidence: 99%

Complete genome sequence analysis of a novel bunyavirus isolated from Paris polyphylla var. yunnanensis

Chen

Anane

Wang

et al. 2021

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A novel negative-stranded (ns) RNA virus tentatively named “Yunnan manyleaf paris rhizome negative-stranded virus 1” (YMPrNSV1), was isolated from a Paris polyphylla var. yunnanensis plant exhibiting leaf chlorosis and mosaic symptoms in Yunnan. Its complete genome sequence was determined using Illumina and Sanger sequencing. The genomes composed of three RNA segments (L, M and S) with each one containing a single open reading frame. Based on sequence identity and the presence of typical bunya-like domains/motifs, the proteins encoded by YMPrNSV1 were predicted to be: RNA-dependent RNA polymerase (RdRp), putative movement protein (MP), and nucleocapsid protein (NP). Sequence comparison analyses showed that the RdRp, MP and NP of YMPrNSV1 are highly similar to those of watermelon crinkle leaf-associated virus 2 (WCLaV-2), with 69.1%, 50.4% and 60.9% amino acid sequence identities respectively. Phylogenetic analysis based on deduced amino acid sequences of RdRp and NP suggested that YMPrNSV1 clustered with coguviruses in a clade, and that WCLaV-2 is the known closely related species to YMPrNSV1. Base on the above results, YMPrNSV1 should be regarded as a new member of genera Coguvirus, within the family Phenuiviridae.

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“…However, virus disease became daily serious with its extension of planting years and expansion of planting area. several viral pathogens, including Paris polyphylla virus X (PPVX) [2], Paris mosaic necrosis virus (PMNV) [3], pepper mild mottle virus (PMMoV) [4], Paris virus 1 (ParV1) [5], Paris virus 2 (ParV2) [6] and chilli veinal mottle virus [7] had been reported infecting the cultivation plants of P. yunnanensis and caused production declining. This paper investigated the viral disease of P. yunnanensis in Yunnan province, determined and characterized the complete genome sequence of a novel potyvirus from plants with leaf mottle.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of a novel potyvirus infecting Paris yunnanensis

Lan

Cao

et al. 2021

Preprint

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The complete genomic sequence of a novel potyvirus from Paris yunnanensis was determined by high-throughput sequencing then confirmed by Sanger sequencing. Its genomic RNA consists 9600 nucleotides (nt) excluding the 3’-terminal poly (A) tail, containing a typical large open reading frame (ORF) of potyviruses and encoding a putative polyprotein of 3098 amino acids (aa). Pairwise comparison analysis showed the virus shares sequence identity with other members of Potyvirus was 53.0–57.8% at genome sequence level, and 39.3–51.2% at polyprotein sequence level. Phylogenetic analysis indicated that the virus was clustered as a single clade within the genus Potyvirus both using nt and aa level. These results suggest that the virus should be considered as a distinct species within the genus Potyvirus, and it was tentatively named as “Paris mottle virus” (PaMoV).

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First Report of Pepper Mild Mottle Virus in Paris polyphylla var. yunnanensis in China

Cited by 21 publications

References 4 publications

Characterization of a novel Tombusviridae species isolated from Paris polyphylla var. yunnanensis

Characterization of a novel Tombusviridae species isolated from Paris polyphylla var. yunnanensis

Complete genome sequence analysis of a novel bunyavirus isolated from Paris polyphylla var. yunnanensis

Identification and characterization of a novel potyvirus infecting Paris yunnanensis

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