Characterization of a novel Tombusviridae species isolated from Paris polyphylla var. yunnanensis

Chen, Lu; Anane, Rex Frimpong; Wang, Zhe; Chen, Zeli; Gao, Like; Geyi, Wen; Zhao, Mingfu

doi:10.1007/s00705-021-05191-y

Cited by 9 publications

(8 citation statements)

References 18 publications

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“…Total nucleic acids were isolated from leaves of the samples using a CTAB-based method ( Li et al, 2008 ) for determination of infection rate of each of ten viruses infecting Paris spp. These viruses included seven viruses (ParPV-3, ParPV-4, ParNV-1, HCRV, LycMoV, PMNV, and ParV1) identified by HTS in this study and three viruses (ChiVMV, PMMoV and PPVX) that were not found in this study but reported previously ( Wen et al, 2019 ; Chen et al, 2021 ; Yang et al, 2022 ). All the samples (20 HTS and 103 additional samples) collected from the four counties were tested individually for each of the target viruses.…”

Section: Methodssupporting

confidence: 60%

“…The spherical particles were measured to be 25–30 nm in diameter ( Figure 2A ), and filamentous particles were 700 ~ 800 nm in length ( Figure 2B ). The results indicated that viruses with spherical shape, besides viruses with filamentous shape ( Dong et al, 2007 ; Lan et al, 2018 ; Wen et al, 2019 ; Chen et al, 2020 , 2021 ; Yang et al, 2022 ), were associated with the viral diseases of P. yunnanensis .…”

Section: Resultsmentioning

confidence: 99%

“…However, virus-like diseases have become serious problems in the crop only after 40 years in cultivation. Several viruses have been previously found in the diseased P. yunnanensis ( Dong et al, 2007 ; Lan et al, 2018 ; Wen et al, 2019 ; Chen et al, 2020 , 2021 ; Yang et al, 2022 ). Aim to insight into pathogens of the viral diseases of various types in P. yunnanensis , morphology, virome analysis and virus surveys were conducted to study species, infection rates and distribution of the viruses in diseased plants in the four growth counties, Mangshi, Lijiang, Tengchong and Lushui, in this study.…”

Section: Discussionmentioning

confidence: 97%

“…PMMoV was detected by RT-PCR. Among them, ParPV-3, ParPV-4 and ParNV-1 are novel, HCRV and LycMoV are first found in P. yunnanensis , while PMNV, ParV1 and PMMoV are known viruses ( Lan et al, 2018 ; Chen et al, 2020 , 2021 ). The HTS and RT-PCR results confirmed the presence of multiple viruses in the diseased plants ( Tables 1 , 2 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, cultivated plants are prone to infection and accumulation of viruses due to high-density planting in the fields and long growth period of 7–10 years. Six viruses, Paris polyphylla virus X (PPVX, genus Potexvirus , family Betaflexiviridae ) ( Dong et al, 2007 ), Paris mosaic necrosis virus (PMNV, genus Potyvirus , Family Potyviridae ) ( Lan et al, 2018 ), Paris virus 1 (ParV1, genus Potyvirus ) ( Chen et al, 2020 ), chilli veinal mottle virus (ChiVMV, genus Potyvirus ) ( Yang et al, 2022 ), pepper mild mottle virus (PMMoV, genus Tobamovirus , family Virgaviridae ) ( Wen et al, 2019 ) and Paris virus 2 (ParV2, genus Tobamovirus ) ( Chen et al, 2021 ), have recently been reported to infect and cause diseases of foliar mottle, necrotic, mosaic, chlorosis and plant stunting in P. yunnanensis .…”

Section: Introductionmentioning

confidence: 99%

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High-throughput sequencing reveals the presence of novel and known viruses in diseased Paris yunnanensis

Lan

Yang

et al. 2022

Front. Microbiol.

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Paris spp. are important medicinal plant and main raw material for many Chinese patent medicines, but viral diseases have became serious problems in cultivation of this group of important medicinal plants in China. In this study, eight viruses were identified in the diseased plants of Paris yunnanensis by high-throughput sequencing (HTS) and RT–PCR. These viruses include three novel viruses (two potyviruses and one nepovirus), Hippeastrum chlorotic ringspot virus (HCRV), Lychnis mottle virus (LycMoV), Paris mosaic necrosis virus (PMNV), Paris virus 1 and pepper mild mottle virus. The three new viruses were tentatively named Paris potyvirus 3 (ParPV-3), Paris potyvirus 4 (ParPV-4), Paris nepovirus 1 (ParNV-1) and their complete genome sequences were determined. Sequence analyses showed ParPV-3 and ParPV-4 shared the highest amino acid (aa) sequence identities of 54.3% to each other and 53.0–57.8% to other known potyviruses. ParNV-1 had aa sequence identities of 28.8–63.7% at protease-polymerase (Pro-Pol) with other nepoviruses. Phylogenetic analyses further support that the three viruses are new members of their corresponding genera. Analyses of the partial sequences of HCRV and LycMoV infecting P. yunnanensis revealed they diverged from existing isolates by aa sequence identities of 97.1% at glycoprotein precursor of HCRV and 93.3% at polyprotein of LycMoV. These two viruses are reported for the first time in Paris spp. A total of 123 field samples collected from P. yunnanensis in four counties of Yunnan, Southwest China were tested by RT–PCR for detecting each of the eight viruses. Results showed that nearly half of the samples were positive for at least one of the eight viruses. Two potyviruses, ParPV-3 (26.8%) and PMNV (24.4%), were predominant and widely distributed in the fields, while other viruses occurred in low rates and/or had limited distribution. This study insights into the virome infecting P. yunnanensis and provides valuable information for diagnosis and control of viral diseases in P. yunnanensis.

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Section: Methodssupporting

confidence: 60%