First report of NDM-5-producing Escherichia coli ST1284 isolated from dog in Bejaia, Algeria

Yousfi, Massilia; Mairi, Assia; Bakour, Sofiane; Touati, Abdelaziz; Hassissen, L.; Hadjadj, Linda; Rolain, Jean‐Marc

doi:10.1016/j.nmni.2015.09.002

Cited by 43 publications

(29 citation statements)

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“…StOLLe et al (2013) studied carbapenemase producing Escherichia coli in dogs harbouring blaCtXM-1, blaOXa-2 and blaTEM-1 genes. YOUSFI et al (2015) reported NDM-5 producing E. coli in a three-year-old German shepherd dog. haraDa et al (2012) screened fecal samples of apparently healthy pups below two months of age from Japanese kennels, with no history of antimicrobial use, and they were found to harbor MDr E. coli isolates, including eSBL producers.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of extensively drug resistant (XDR), extended spectrum beta-lactamases (ESBL) and New Delhi Metallo beta-lactamase-1 (blaNDM1) producing Escherichia coli isolated from a male dog - a case report

et al. 2018

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Molecular characterization of extensively drug resistant (XDR), extended spectrum beta-lactamases (ESBL)and New Delhi Metallo beta-lactamase-1 (blaNDM1) producing Escherichia coli isolated from a male dog -a case report. Vet. arhiv 88, 139-148, 2018.aBSTRaCT In this article, we report the molecular characterization of extensively drug resistant (XDr), extended spectrum, class C beta-lactamases and NDM-1 carbapenemase producing E. coli, isolated from the scrotal fluid of a 3-year-old male dog. In an antibiotic susceptibility test the E. coli isolate was susceptible only to tigecycline and resistant to all clinically applicable antibiotics tested in the study. the minimum inhibitory concentration (MIC) of meropenem, cefotaxime and cefepime was 256, 128 and 64 µg/mL, respectively. On genotypic screening by PCr, the isolate was positive for blaNDM, blaCtX-M, blaampC, blateM and sul1 genes. the isolate was a eSBL, ampC and metalo beta-lactamase producer. On molecular pathotyping, the isolate harbored the Shiga toxin producing gene (Stx2). the extensively drug resistant, carbapenem resistant and eSBL producing E. coli constitutes a major public health concern, since there is a great chance of dissemination of resistance genes to humans due to the close association of humans and companion animals. to the best of our knowledge, this is the first report of blaNDM1 isolated from a dog in India.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of extensively drug resistant (XDR), extended spectrum beta-lactamases (ESBL) and New Delhi Metallo beta-lactamase-1 (blaNDM1) producing Escherichia coli isolated from a male dog - a case report

et al. 2018

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“…However, the pilgrims acquired not only Acinetobacter species but also E. coli isolates with bla NDM-5 . The bla NDM-5 resistance gene has been reported in E. coli and Klebsiella pneumoniae isolates from hospitalized patients in several countries, including Denmark, South Korea, India, Algeria, the United Kingdom, Japan, and Spain (32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44), and from a dog in Algeria (45). One of our E. coli isolates possessed an ST2659 sequence previously recovered between January 2012 and February 2013 from urine and blood specimens from patients in the University Hospital of Annaba (east Algeria) (34).…”

Section: Discussionmentioning

confidence: 99%

Acquisition of a High Diversity of Bacteria during the Hajj Pilgrimage, Including Acinetobacter baumannii with bla _OXA-72 and Escherichia coli with bla _NDM-5 Carbapenemase Genes

Leangapichart

Gautret

Griffiths

et al. 2016

Antimicrob Agents Chemother

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bPilgrims returning from the Hajj (pilgrimage to Mecca) can be carriers of multidrug-resistant bacteria (MDR). Pharyngeal and rectal swab samples were collected from 98 pilgrims before and after they traveled to the Hajj in 2014 to investigate the acquisition of MDR bacteria. The bacterial diversity in pharyngeal swab samples was assessed by culture with selective media. There was a significantly higher diversity of bacteria in samples collected after the return from the Hajj than in those collected before (P ‫؍‬ 0.0008). Surprisingly, Acinetobacter baumannii strains were isolated from 16 pharyngeal swab samples (1 sample taken The Hajj (pilgrimage to Mecca) is the world's largest annual mass gathering which brings together millions of people from many countries to perform rituals in Saudi Arabia. Interaction and close contact are important factors for the transmission and dissemination of infectious diseases among pilgrims. Several airborne infectious diseases have been reported during the Hajj, including meningitis, flu, and tuberculosis (1). Common respiratory pathogens have been reported among pilgrims with clinical pneumonia, including Haemophilus influenzae, Streptococcus pneumoniae, Moraxella catarrhalis, Mycobacterium tuberculosis, Klebsiella spp., Pseudomonas aeruginosa, Staphylococcus aureus, and Acinetobacter baumannii (2, 3) Moreover, high rates of acquisition of rhinovirus and Streptococcus pneumoniae as well as coronavirus E229 by pilgrims were reported during the 2013 Hajj (4). With the global spread of antibiotic-resistant bacteria, international travelers are potentially at risk of acquiring multidrugresistant (MDR) bacteria and antibiotic resistance (AR) genes and of transferring these to other people when they return to their home countries (5, 6). This has been well documented, with reports describing the acquisition of NDM-1-producing bacteria in travelers returning from the Indian subcontinent to the United Kingdom (7) and KPC-producing bacteria in French patients who had traveled to the United States (8). We recently reported the acquisition of colistin-resistant Salmonella enterica serotype Newport in rectal swab samples taken from French pilgrims during the 2013 Hajj (6, 9). Acinetobacter species, particularly MDR A. baumannii strains, are primarily associated with hospital-acquired pneumonia. However, several studies have reported cases of community-acquired A. baumannii pneumonia, such as in Australia during the rainy season, for which the source and mode of transmission remain unknown (10). We aimed to determine, using pharyngeal and rectal swabs, whether pilgrims might have acquired MDR bacteria during the 2014 Hajj. Here we report for the first time the acquisition of cephalosporin-and imipenem-resistant A. baumannii and Escherichia coli among pilgrims returning from Saudi Arabia. MATERIALS AND METHODSStudy design. Data were obtained between 19 September and 12 October 2014 (24 days) from a cohort of pilgrims that was traveling from France to Mecca in Saudi Arabia with a specialize...

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“…The NDM-5-encoding gene bla was first identified in an Escherichia coli strain recovered from a patient in the United Kingdom in 2011 (3). Since then, bla was identified in many countries, such as Algeria (4)(5)(6), the United States (7), Australia (8), China (9)(10)(11)(12), Denmark (13), Japan (14), India (15), and the United Kingdom (3). The widespread occurrence of NDM-5 in recent years should arouse our attention.…”

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confidence: 99%

Various Sequence Types of Escherichia coli Isolates Coharboring bla _NDM-5 and mcr-1 Genes from a Commercial Swine Farm in China

Kong

Lei

et al. 2017

Antimicrob Agents Chemother

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Sixteen different sequence types (STs) of Escherichia coli isolates from a commercial swine farm in China were confirmed to coharbor the carbapenem resistance gene bla and the colistin resistance gene mcr-1. Whole-genome sequencing revealed that bla NDM-5 and mcr-1 were located on a 46-kb IncX3 plasmid and a 32-kb IncX4 plasmid, respectively. The two plasmids can transfer together with a low fitness cost, which might explain the presence of various STs of E. coli coharboring bla NDM-5 and mcr-1.KEYWORDS Escherichia coli, carbapenems, bla NDM-5 , colistin, mcr-1, fitness cost C arbapenemase-producing Enterobacteriaceae has become a major public health threat around the world (1). The recently identified carbapenemase New Delhi metallo-␤-lactamase confers resistance to all ␤-lactam antimicrobials except monobactam (2). The NDM-5-encoding gene bla NDM-5 was first identified in an Escherichia coli strain recovered from a patient in the United Kingdom in 2011 (3). Since then, bla was identified in many countries, such as Algeria (4-6), the United States (7), Australia (8), China (9-12), Denmark (13), Japan (14), India (15), and the United Kingdom (3). The widespread occurrence of NDM-5 in recent years should arouse our attention. Colistin is a critically important medication for humans in the treatment of carbapenemaseproducing Enterobacteriaceae, and it has been widely used in veterinary medicine in China (16,17). The first plasmid-mediated colistin resistance gene, mcr-1, was reported in E. coli in 2015 (18). In a short period, colistin-resistant E. coli carrying the mcr-1 gene were reported worldwide (19,20). Recently, mcr-1 was reported to coexist with bla NDM (21-23) and bla CTX-M (24), which brought great challenges for the treatment of bacterial infection. In the present study, we are the first to report the presence of isolates of various sequence types of E. coli coharboring bla NDM-5 and mcr-1 genes from a commercial pig farm in China.A total of 105 anal swabs samples from swine were collected from a commercial pig farm on 1 October 2015 in Sichuan province. E. coli strains were selected by eosinmethylene blue agar, and only 1 isolate was picked up from each sample. All 105 isolates were identified by BD Phoenix 100 diagnostic systems (Sparks, MD). Sixty-four strains were nonsusceptible to imipenem and polymyxin B, identified by the agar dilution method according to Clinical and Laboratory Standards Institute guidelines (25). Isolates were divided into 16 different clones by pulsed-field gel electrophoresis after XbaI digestion according to the standard PulseNet conditions (26) (Fig. 1). Phy-

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First report of NDM-5-producing Escherichia coli ST1284 isolated from dog in Bejaia, Algeria

Cited by 43 publications

References 9 publications

Molecular characterization of extensively drug resistant (XDR), extended spectrum beta-lactamases (ESBL) and New Delhi Metallo beta-lactamase-1 (blaNDM1) producing Escherichia coli isolated from a male dog - a case report

Molecular characterization of extensively drug resistant (XDR), extended spectrum beta-lactamases (ESBL) and New Delhi Metallo beta-lactamase-1 (blaNDM1) producing Escherichia coli isolated from a male dog - a case report

Acquisition of a High Diversity of Bacteria during the Hajj Pilgrimage, Including Acinetobacter baumannii with bla _OXA-72 and Escherichia coli with bla _NDM-5 Carbapenemase Genes

Various Sequence Types of Escherichia coli Isolates Coharboring bla _NDM-5 and mcr-1 Genes from a Commercial Swine Farm in China

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First report of NDM-5-producing Escherichia coli ST1284 isolated from dog in Bejaia, Algeria

Cited by 43 publications

References 9 publications

Molecular characterization of extensively drug resistant (XDR), extended spectrum beta-lactamases (ESBL) and New Delhi Metallo beta-lactamase-1 (blaNDM1) producing Escherichia coli isolated from a male dog - a case report

Molecular characterization of extensively drug resistant (XDR), extended spectrum beta-lactamases (ESBL) and New Delhi Metallo beta-lactamase-1 (blaNDM1) producing Escherichia coli isolated from a male dog - a case report

Acquisition of a High Diversity of Bacteria during the Hajj Pilgrimage, Including Acinetobacter baumannii with bla OXA-72 and Escherichia coli with bla NDM-5 Carbapenemase Genes

Various Sequence Types of Escherichia coli Isolates Coharboring bla NDM-5 and mcr-1 Genes from a Commercial Swine Farm in China

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Acquisition of a High Diversity of Bacteria during the Hajj Pilgrimage, Including Acinetobacter baumannii with bla _OXA-72 and Escherichia coli with bla _NDM-5 Carbapenemase Genes

Various Sequence Types of Escherichia coli Isolates Coharboring bla _NDM-5 and mcr-1 Genes from a Commercial Swine Farm in China