Molecular characterization of extensively drug resistant (XDR), extended spectrum beta-lactamases (ESBL) and New Delhi Metallo beta-lactamase-1 (blaNDM1) producing Escherichia coli isolated from a male dog - a case report

Pruthvishree, B.S.; Kumar, O.R. Vinodh; Sivakumar, M.; Tamta, Shikha; Ramanjeneya, Sunitha; Sinha, Dharmendra Kumar; Singh, Bhoj Raj

doi:10.24099/vet.arhiv.160924

Cited by 10 publications

(5 citation statements)

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“…In this study, the ESBL-producing E. coli showed resistance to clinically important antibiotics such as cephalosporins, fluoroquinolones, and aminoglycosides and jeopardize the effective prevention and treatment of various bacterial infections [21]. The present study revealed that all the ESBL-producing E. coli were 100% resistant to cefotaxime and ceftazidime and were concordance with others [11][12][13]. This study reported that the entire ESBL-positive E. coli from piglets as MDR, similarly, in a study from China reported MDR phenotype in total ESBL-producing E. coli isolated from pigs [22].…”

Section: Discussionsupporting

confidence: 81%

“…The emergence and spread of ESBLs producing E. coli in food-producing animals is a major public health issue worldwide [11][12][13][14][15]. Piglets and farm workers harbored ESBL E. coli, the percentage of cefotaxime and ceftazidime resistance is higher among isolates as compared to ciprofloxacin and chloramphenicol indicates the abundant use of beta-lactam and cephalosporin antimicrobials in piglets and human [5,15].…”

Section: Discussionmentioning

confidence: 99%

“…Reports suggest the possibility of transferring ESBL-producing E. coli from farm workers to food animal and vice versa [6][7][8][9][10]. Multidrug-resistant E. coli are reported in food animals and pet animals of India [11][12][13][14][15]. Besides, food animals may act as a reservoir for ESBLproducing strains and foods as a vehicle for the transfer of β-lactam-producing bacteria [16].…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial resistance pattern of extended-spectrum β-lactamase-producing Escherichia coli isolated from fecal samples of piglets and pig farm workers of selected organized farms of India

et al. 2020

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Background and Aim: Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli are gradually increasing worldwide and carry a serious public threat. This study aimed to determine the antimicrobial resistance pattern of ESBL-producing E. coli isolated from fecal samples of piglets and pig farm workers. Materials and Methods: Fecal samples from <3-month-old piglets (n=156) and farm workers (n=21) were processed for the isolation of ESBL-producing E. coli in MacConkey agar added with 1 μg/mL of cefotaxime. E. coli (piglets=124; farm workers=21) were tested for ESBL production by combined disk method and ESBL E-strip test. Each of the ESBL-positive isolate was subjected to antibiotic susceptibility testing. The ESBL-producing E. coli were further processed for genotypic confirmation to CTX-M gene. Results: A total of 55 (44.4%, 55/124) and nine (42.9%, 9/21) ESBL-producing E. coli were isolated from piglets and farm workers, respectively. Antibiotic susceptibility testing of the ESBL-positive E. coli isolates from piglets and farm workers showed 100% resistance to ceftazidime, cefotaxime, cefotaxime/clavulanic acid, ceftazidime/clavulanic acid, and cefpodoxime. A proportion of 100% (55/55) and 88.9% (8/9) ESBL-positive E. coli were multidrug resistance (MDR) in piglets and farm workers, respectively. On genotypic screening of the ESBL E. coli isolated from piglets (n=55), 15 were positive for the blaCTX-M gene and of the nine ESBL E. coli from farm workers, none were positive for the blaCTX-M gene. Conclusion: Although there was no significant difference in isolation of ESBL-producing E. coli between piglets and farm workers, the ESBL-positive E. coli from piglets showed relatively higher MDR than farm workers.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antimicrobial resistance pattern of extended-spectrum β-lactamase-producing Escherichia coli isolated from fecal samples of piglets and pig farm workers of selected organized farms of India

et al. 2020

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“…Several chromosomal and plasmid encoded carbapenemases such as KPC, MBL, Verona integron-encoded MBL (VIM), Imipenem-resistant Pseudomonas (IMP), New Delhi MBL (NDM) and Oxacillinase (OXA) -48, have been documented throughout the world, that could easily be transferred between different enterobacterial species (Stolle et al, 2013;Abraham et al, 2014;Netikul and Kiratisin, 2015). Today, the population of companion animals such as dog and cat is substantially increasing in modern society and the major mechanism of antibiotic resistance among bacterial agents from pets and humans is the exchange of resistance genes (Pruthvishree et al, 2018). Therefore, firm control on infection and surveillance measures combined with prudent use of antibiotics in both human and veterinary practice is essential to reduce the spread of resistance against carbapenems.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic Characterisation of Klebseilla pneumoniae Carbapenemase and Metallo Beta-lactamase in Carbapenem Resistant Gram-negative Bacteria

Thresia¹,

Sankar²,

Joseph³

et al. 2021

IJAR

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Background: Antibiotic resistance is an emerging concern in the therapy of clinical infections worldwide. Previous studies conducted in our laboratory have confirmed an increase in the prevalence of extended spectrum beta-lactamase (ESBL) among the Gram-negative bacterial pathogens associated with dogs, which could act as a potential source for the transfer of these resistant pathogens or their genetic determinants to human. Since carbapenems are the last resort drugs against these resistant pathogens, the study was aimed to isolate and characterise carbapenem resistance among Escherichia coli (E. coli), Klebsiella pneumoniae (K. pneumoniae) and Pseudomonas aeruginosa (P. aeruginosa) associated with common clinical infections in dogs.Methods: A total of 100 samples were collected from lesional skin, urine and anterior vagina of dogs presented to the Veterinary Hospitals of Kerala Veterinary and Animal Sciences University at Mannuthy and Thrissur. The samples were cultured onto Brain Heart Infusion Agar (BHIA), Eosin Methylene Blue (EMB) and Mac Conkey (MAC) for isolation of bacteria. Identification of the isolates was performed based on cultural, morphological and biochemical characteristics. The isolates were subjected to antimicrobial susceptibility test (ABST) against the 12 commonly used beta-lactam and non–beta-lactam group of antibiotics by disc diffusion method and further subjected to screening for ESBL double disc diffusion method. Carbapenem-resistant isolates were subjected to phenotypic confirmatory test for carbapenemase production employing Imipenem-EDTA and Ertapenem-boronic acid minimum inhibitory concentration (MIC) strip method.Result: Forty four Gram-negative bacterial isolates obtained were viz., E. coli (30), K. pneumonia (11) and P. aeruginosa (3) from the 100 samples. Apart from these, other isolates obtained were Staphylococcus spp. (53) and Bacillus spp. (2). All the Gram-negative isolates were subjected to ABST employing 12 common antibiotics belonging to beta-lactam and non-beta-lactam groups. Multidrug resistance (MDR) could be observed in 28 E. coli, 11 K. pneumoniae and three P. aeruginosa isolates. All the 42 MDR isolates showed positive results for ESBL production. A total of 14 isolates out of the 44 Gram-negative bacilli were found to be resistant to carbapenem either to imipenem, meropenem or ertapenem. Among the 14 Gram-negative isolates, nine turned out to be positive for metallo-beta-lactamase (MBL) and none for K. penumoniae carbapenemase (KPC) on phenotypic confirmatory test for detecting major carbapenemase enzymes. The present study documented that Gram- negative bacteria like E. coli, K. pneumoniae and P. aeruginosa isolated from dogs are showing an increase rate of resistance against carbapenems which are the last resort drugs against ESBL producers. Hence, there is an urgent need to curb the irrational and excessive use of antibiotics in veterinary sector.

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“…). Reports were available in India on ESBL producing and carbapenem resistant E. coli harboring virulence genes in animals(Pruthvishree et al 2017;Nirupama et al 2018;Pruthvishree et al 2018). Plasmid replicon typing of the three blaNDM-5gene-positive E. coli revealed that the NDM gene was on Incl1 plasmid in two isolates (SB27/ 17-CRE, SB28/ 12-CRE) and IncF plasmid was on one isolate (SB28/ 16-CRE).…”

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confidence: 99%

Multi-drug Resistant (MDR), Extended Spectrum Beta-lactamase (ESBL) and New Delhi Metallo Beta-lactamase (NDM) Producing Escherichia Coli in Rescued Sloth Bears (Melursus Ursinus), India

Vinodhkumar¹,

Karikalan²,

Ilayaraja³

et al. 2021

Preprint

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The study reports the MDR, ESBL, and NDM producing Escherichia coli (CRE) isolated from the rescued sloth bear (Melursus ursinus), India. The faecal samples of adult rescued sloth bear (n=21) were collected from a rescue center located in India during 2015-2016 and processed for isolation and antibacterial susceptibility pattern of E. coli. 45E. coli isolates were recovered, and on phenotypic screening, 23 were MDR, 17 were ESBL producers, and five were carbapenem-resistant (CR). The MDR isolates carried beta-lactamase, chloramphenicol, aminoglycosides, tetracycline, fluroquinone, and sulphadimidine resistance genes. All the phenotypic ESBL producing isolates had blaCTX-M genes. On genotypic screening, three CRE (60.0 %, 3/5) were positive for blaNDM carbapenemase gene. Efflux pump-mediated carbapenem resistance was noticed in two CRE isolates (40.0 %, 2/5).The CRE also isolates co-harbored AMR genes like blaTEM-1, blaAmpC, qnrA, qnrB, qnrS, tetA, tetB and sulI. Virulence screening of the resistant isolates revealed the presence of Stx1, Stx2, eae, hlyA genes.Plasmid incompatibility (Inc) typesof three NDM positive isolates revealed that two isolates blaNDM-5 gene on Incl1 an one isolate on IncF plasmid. Apart from NDM genes, the plasmids also carried tetracycline, beta-lactamase and quinolone resistance genes. The plasmid multilocus sequence typing (pMLST) of the E. coli Incl1 plasmid showed the Sequence Type (ST) 297.This appears to be the first report of multi-drug resistant, extended spectrum beta-lactamase (ESBL) producing and blaNDM-5geneson Incl1 and IncF plasmids in rescued sloth bear.

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Molecular characterization of extensively drug resistant (XDR), extended spectrum beta-lactamases (ESBL) and New Delhi Metallo beta-lactamase-1 (blaNDM1) producing Escherichia coli isolated from a male dog - a case report

Cited by 10 publications

References 26 publications

Antimicrobial resistance pattern of extended-spectrum β-lactamase-producing Escherichia coli isolated from fecal samples of piglets and pig farm workers of selected organized farms of India

Antimicrobial resistance pattern of extended-spectrum β-lactamase-producing Escherichia coli isolated from fecal samples of piglets and pig farm workers of selected organized farms of India

Phenotypic Characterisation of Klebseilla pneumoniae Carbapenemase and Metallo Beta-lactamase in Carbapenem Resistant Gram-negative Bacteria

Multi-drug Resistant (MDR), Extended Spectrum Beta-lactamase (ESBL) and New Delhi Metallo Beta-lactamase (NDM) Producing Escherichia Coli in Rescued Sloth Bears (Melursus Ursinus), India

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