First Report of Japanese Pear Black Spot Caused by<i>Alternaria kikuchiana</i>in France

Baudry, A.; Morzières, J. P.; Larue, P.

doi:10.1094/pd-77-0428b

Cited by 17 publications

(4 citation statements)

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“…Alternaria kikuchiana is the pathogen of pear black spot disease. It infects the fruits, leaves and shoots of different varieties of pear trees, causing the leaves of pear trees to fall, early fruit drop, fruit decay and shedding, post-harvest decay, etc [24]. Phomopsis sp.…”

Section: Discussionmentioning

confidence: 99%

Derivatization of Natural Compound β-Pinene Enhances Its In Vitro Antifungal Activity against Plant Pathogens

Shi

Wang

et al. 2019

Molecules

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Background: The development of new antifungal agents has always been a hot research topic in pesticide development. In this study, a series of derivatives of natural compound β-pinene were prepared, and the antifungal activities of these derivatives were evaluated. The purpose of this work is to develop some novel molecules as promising new fungicides. Methods: Through a variety of chemical reactions, β-pinene was transformed into a series of β-pinene-based derivatives containing amide moieties and acylthiourea moieties. The antifungal activities of these derivatives against five plant pathogens including Colletotrichum gloeosporioides, Fusarium proliferatum, Alternaria kikuchiana, Phomopsis sp. and Phytophthora capsici were tested; preliminary structure–activity relationship was discussed. Results: Some derivatives exhibited moderate or significant antifungal activity due to the fusion of the amide moiety or the acylthiourea moiety with the pinane skeleton. The structure–activity relationship analysis showed that the fluorine atom and the strong electron withdrawing nitro group, or trifluoromethyl group on the benzene ring of the derivatives had a significant effect on the improvement of the antifungal activity against Colletotrichum gloeosporioides, Fusarium proliferatum, Alternaria kikuchiana and Phomopsis sp. Meanwhile, the introduction of an ethyl group at the meta-position on the benzene ring of the derivatives could improve the antifungal activity against Phytophthora capsici. Compounds 4e, 4h, 4q, 4r exhibited broad-spectrum antifungal activity against the tested strains. Compound 4o had significant antifungal activity against Phytophthora capsici (IC50 = 0.18 μmol/L). These derivatives were expected to be used as precursor molecules for novel pesticide development in further research.

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Section: Discussionmentioning

confidence: 99%

Derivatization of Natural Compound β-Pinene Enhances Its In Vitro Antifungal Activity against Plant Pathogens

Shi

Wang

et al. 2019

Molecules

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“…Kusaba and Tsuge (1995) went as far as to conclude that the small-spored species are all intraspecific variants of one species, A. alternata. However, species such as A. gaisen and A. yaliinficiens that, prior to 1992 and 1995, respectively, were geographically limited to Asia (Kohmoto et al 1992;Baudry et al 1993;Roberts 2005) are supported in DNA fingerprint analyses and metabolite profiles that have supported morphological segregation of several species-groups and are generally congruent with morphological character states (Roberts et al 2000;Hong et al 2005;Peever et al 1999;Andersen and Thrane 1996;Andersen et al 2001Andersen et al , 2002. We used subtractive hybridization to isolate and characterize sporulation-associated expressed sequence tags (ESTs) produced at the onset of conidial chain formation in response to light exposure, which we determined is required for conidiation in A. gaisen RGR91.0166 but not in A. alternata RGR91.0102 (data not shown).…”

Section: Introductionmentioning

confidence: 99%

Differential gene expression in Alternaria gaisen exposed to dark and light

2011

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Character states during sporulation have been used to segregate and describe many small-spored species of Alternaria, but some are not supported by published phylogenetic analyses. The conidiation response of Alternaria gaisen was characterized by selective subtractive hybridization of cDNA produced from cultures of A. gaisen grown either in total darkness or in total darkness followed by scarification and 24 h exposure to light. Transcripts or their translation products were identified using BLAST. Multiple transcripts with similarity to ORF-1 of the AM-toxin gene were obtained from the light library. L152 is a full reading frame EST in the light library whose ORF translation has similarity to the conserved domain aegerolysin (pfam06355). A set of 11 ex-type or representative isolates including A. alternata, A. gaisen, A. yaliinficiens, A. arborescens, A. tenuissima and A. brassicicola were resolved by UPGMA analysis of a partial genomic sequence (415-425 base pairs) of L152, but were not resolved by a similar analysis of ITS sequences. Furthermore, the resolved lineages of the L152 dataset were reflective of the diversity previously hypothesized by morphological evaluations of sporulation patterns. Although the ITS rDNA sequence region is generally accepted as the most likely candidate for fungal barcoding, the analysis of L152 sequences presented here resolved closely related species or species groups where other loci, including ITS, have not. Based on these results, the sequences of putative aegerolysin homologs were variable, parsimonyinformative and warrant additional analyses with a broader isolate set including related genera and species.

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“…A. gaisen had been reported as a causal agent of black spot disease in Japanese pears (Pyrus pyrifolia) previously discovered only in Japan, Korea, and China (Simmons and Roberts, 1993). However, now, it has been discovered in various hostswintersweet (Chimonanthus praecox), rice (Oryzae sativa), gerbera daisy (Gerbera jamesonii), and watermelon-of many countries including France, Australia, China, Pakistan, and the US (Akhtar et al, 2014;Baudry et al, 1993;Ma et al, 2021;Perveen et al, 2018;Tian et al, 2020). Additional study will be necessary to determine whether A. gaisen can be another predominant species of Alternaria leaf blight of watermelon in Korea.…”

Section: Discussionmentioning

confidence: 99%

Incidence of Alternaria Species Associated with Watermelon Leaf Blight in Korea

Kwon

Jeong

et al. 2021

Plant Pathol J

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Alternaria leaf blight is one of the most common diseases in watermelon worldwide. In Korea, however, the Alternaria species causing the watermelon leaf blight have not been investigated thoroughly. A total of 16 Alternaria isolates was recovered from diseased watermelon leaves with leaf blight symptoms, which were collected from 14 fields in Korea. Analysis of internal transcribed spacer (ITS) region, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and RNA polymerase II second largest subunit (RPB2) were not competent to differentiate the Alternaria isolates. On the contrary, analysis of amplicon size of the histone H3 (HIS3) gene successfully differentiated the isolates into three Alternaria subgroups, and further sequence analysis of them identified three Alternaria spp. Alternaria tenuissima, A. gaisen, and A. alternata. Representative Alternaria isolates from three species induced dark brown leaf spot lesions on detached watermelon leaves, indicating that A. tenuissima, A. gaisen, and A. alternata are all causal agents of Alternaria leaf blight. Our results indicate that the Alternaria species associated watermelon leaf blight in Korea is more complex than reported previously. This is the first report regarding the population structure of Alternaria species causing watermelon leaf blight in Korea.

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First Report of Japanese Pear Black Spot Caused byAlternaria kikuchianain France

Cited by 17 publications

References 0 publications

Derivatization of Natural Compound β-Pinene Enhances Its In Vitro Antifungal Activity against Plant Pathogens

Derivatization of Natural Compound β-Pinene Enhances Its In Vitro Antifungal Activity against Plant Pathogens

Differential gene expression in Alternaria gaisen exposed to dark and light

Incidence of Alternaria Species Associated with Watermelon Leaf Blight in Korea

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