First report of Iris yellow spot virus in Costa Rica

Abstract

Iris yellow spot virus (IYSV), an emerging disease of onion crops, was identified by transmission electron microscopy, enzyme linked immunosorbent assay, and reverse transcription polymerase chain reaction in Costa Rica. Onion plants had straw-colored, elongated lesions and tip dieback. Costa Rican IYSV nucleocapsid partial sequences (15 isolates) grouped with isolates from North and Central America and from New Zealand.

Detection of Plantago asiatica mosaic virus in lily hybrid plants (Lilium spp.) in Costa Rica grown from imported bulbs

“…(Kumar et al 2016) with 5000 permutations and the rate variation among sites was modeled with a gamma distribution (shape parameter = 1). Blue font values above the diagonal are standard error estimates Small leaf segments (1 × 2 mm) were harvested around chlorotic and necrotic lesions on leaves of one greenhouse lily sample (T188) and were processed for transmission electron microscopy (TEM) as previously described (Montero-Astúa et al 2017). We detected individual flexuous-rod shaped particles scattered in the cytoplasm (Fig.…”