Detection of Plantago asiatica mosaic virus in lily hybrid plants (Lilium spp.) in Costa Rica grown from imported bulbs

Montero-Astúa, Mauricio; Garita, Laura; Vásquez, Elena Ramos; Moreira, Lisela

doi:10.1007/s13314-017-0281-2

Cited by 6 publications

(4 citation statements)

References 27 publications

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“…To control plant viral diseases and produce healthy plants free of viruses, the indexing process in lilies focuses on both axillary and apical meristems of the parent plant, the resulting plant, as well as the plant coming from the seed [30,31]. Viruses can be transmitted through the parent plant or the seed itself to the new plant, making indexing essential.…”

Section: Methods To Eliminate Viruses From Lily Tissuesmentioning

confidence: 99%

“…This is particularly crucial for viruses that can be vertically transmitted from the parent plant to the seed. The specific indexing practices may vary based on the type of virus, the plant species, and the desired level of assurance for producing healthy plants free of viruses [30][31][32]. Additionally, producing healthy seeds that have been indexed and shown to be free of known viruses is a common method for controlling plant viral diseases.…”

Section: Methods To Eliminate Viruses From Lily Tissuesmentioning

confidence: 99%

“…Indexing methods involve selecting a representative subset of seeds from the batch for testing, typically based on statistical principles to ensure accuracy. Additionally, indexing includes the use of resistant indicator plants or acquiring seed, cuttings, or plants from certified virus-free sources [30][31][32][33]. Furthermore, ongoing investigations are exploring the elimination of lily virus through tissue culture-based methods (Table 1).…”

Section: Methods To Eliminate Viruses From Lily Tissuesmentioning

confidence: 99%

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Advancements in Lily Viruses Management: Challenges and Solutions in Elimination and Detection

Gong

Dusengemungu

et al. 2023

Horticulturae

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Lilies are important crops that are commonly used as cut flowers (Lilium spp.) and edible bulb crops (Lilium davidii var. unicolor). However, virus infections can significantly impact the quantity and quality of lily production. Various methods have been developed to eliminate viruses in lilies, including in vitro culture and virus detection techniques. Meristem culture is the most effective method, which can be combined with other techniques such as thermotherapy and chemotherapy. Nonetheless, virus elimination is affected by several factors, including cultivar, explants used, virus type, and duration of treatments. Efficient diagnostic methods, such as serological and molecular techniques, have been developed to detect viral infections in lilies, including enzyme-linked immunosorbent assay (ELISA) and real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR). However, cross-contamination and multiple-virus contamination can lead to unreliable results, and more sophisticated protocols and systems have been developed to address these issues. The objective of this review is to provide a comprehensive overview of the development of lily virus eradication, detection strategies, challenges, and solutions associated with these procedures, and how more sophisticated approaches such as multiplex RT-PCR, indirect ELISA (ID-ELISA), immunocapture RT-PCR (IC-RT-PCR), and immunochromatographic test strips (ICSs) can alleviate some of these setbacks.

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Section: Methods To Eliminate Viruses From Lily Tissuesmentioning

confidence: 99%

Section: Methods To Eliminate Viruses From Lily Tissuesmentioning

confidence: 99%

Section: Methods To Eliminate Viruses From Lily Tissuesmentioning

confidence: 99%

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Advancements in Lily Viruses Management: Challenges and Solutions in Elimination and Detection

Gong

Dusengemungu

et al. 2023

Horticulturae

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“…This was reported first in the Netherlands (EPPO, 2011) and in Chile in 2013 (Vidal et al, 2016). However, soon after its first known occurrence in commercial lily hybrids, PlAMV was detected in commercial lily stocks in many other countries in Europe and around the world (Anderson et al, 2013; Chen et al, 2013; Hammond et al, 2015; Harju et al, 2018; Kim et al, 2015; Li et al, 2017; Montero‐Astúa et al, 2017; Pájtli et al, 2015; Parrella et al, 2015; Xu et al, 2017). Interestingly, the isolates from commercial lily products (“European‐like” isolates) have extremely closely related sequences, quite distinct from those obtained from edible lilies in Japan (Hammond & Reinsel, 2018; Komatsu et al, 2017; Ozeki et al, 2006).…”

Section: Host Range Transmission and Symptomsmentioning

confidence: 99%

Plantago asiatica mosaic virus: An emerging plant virus causing necrosis in lilies and a new model RNA virus for molecular research

Komatsu

2022

Molecular Plant Pathology

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Taxonomy: Plantago asiatica mosaic virus belongs to the genus Potexvirus in the family Alphaflexiviridae of the order Tymovirales. Virion and genome properties: Plantago asiatica mosaic virus (PlAMV) has flexuous virions of approximately 490-530 nm in length and 10-15 nm in width. The genome of PlAMV consists of a single-stranded, positive-sense RNA of approximately 6.13 kb. It contains five open reading frames (ORFs 1-5), encoding a putative viral polymerase (RdRp), movement proteins (triple gene block proteins, TGBp1-3), and coat protein (CP), respectively. Host range: PlAMV has an exceptionally wide host range and has been isolated from various wild plants, including Plantago asiatica, Nandina domestica, Rehmannia glutinosa, and other weed plants. Experimentally PlAMV can infect many plant species including Nicotiana benthamiana and Arabidopsis thaliana. It also infects ornamental lilies and frequently causes severe necrotic symptoms. However, host range varies depending on isolates, which show significant biological diversity within the species. Genome diversity: PlAMV can be separated into five clades based on phylogenetic analyses; nucleotide identities are significantly low between isolates in the different clades. Transmission: PlAMV is not reported to be transmitted by biological vectors. Virions of PlAMV are quite stable and it can be transmitted efficiently by mechanical contact. Disease symptoms: PlAMV causes red-rusted systemic necrosis in ornamental lilies, but it shows much weaker, if any, symptoms in wild plants such as P. asiatica. Control: Control of the disease caused by PlAMV is based mainly on rapid diagnosis and elimination of the infected bulbs or plants.

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Lilium spp. (Lilium longiflorum, L. lancifolium, L. tsingtauense)

Sastry¹,

Mandal²,

Scott³

et al. 2019

Encyclopedia of Plant Viruses and Viroids

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Detection of Plantago asiatica mosaic virus in lily hybrid plants (Lilium spp.) in Costa Rica grown from imported bulbs

Cited by 6 publications

References 27 publications

Advancements in Lily Viruses Management: Challenges and Solutions in Elimination and Detection

Advancements in Lily Viruses Management: Challenges and Solutions in Elimination and Detection

Plantago asiatica mosaic virus: An emerging plant virus causing necrosis in lilies and a new model RNA virus for molecular research

Lilium spp. (Lilium longiflorum, L. lancifolium, L. tsingtauense)

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