First report of <i>Tomato brown rugose fruit virus</i> in tomato in the United Kingdom

Skelton, A.; Buxton‐Kirk, A.; Ward, R.; Harju, V.; Frew, L.; Fowkes, A.; Long, M.; Negus, A.; Forde, S.; Adams, Ian; Pufal, Hollie; McGreig, Sam; Weekes, Rebecca; Fox, Adrian

doi:10.5197/j.2044-0588.2019.040.012

Cited by 60 publications

(45 citation statements)

References 4 publications

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“…described and used for Sanger sequence based ToBRFV verification [2,3,10,11,13,16,37]. Public sequences obtained with Sanger sequencing cover either the 5' end of the small replicase subunit, the 3' end of the small replicase subunit, the 3' end of RdRp, the partial MP or partial CP (S4 Fig).…”

Section: Plos Onementioning

confidence: 99%

“…Seed transmission of ToBRFV is presumed [7], and bumblebees (Bombus terrestris) have been reported as vectors for ToBRFV and potentially contribute to the spread of the disease [8]. Following the initial findings in Jordan and Israel, the virus was reported from the State of Palestine [9], Mexico [10], the United States of America [11], Germany [12], and Italy [13] in 2018, and Turkey [14], China [15], the United Kingdom [16], the Netherlands [17], Greece [18], and Spain [19] in 2019. In 2020, ToBRFV outbreaks were reported from France [20] and Egypt [21].…”

Section: Introductionmentioning

confidence: 99%

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Real-time tracking of Tomato brown rugose fruit virus (ToBRFV) outbreaks in the Netherlands using Nextstrain

Vossenberg¹,

Visser²,

Bruinsma³

et al. 2020

PLoS ONE

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Tomato brown rugose fruit virus (ToBRFV) is a Tobamovirus that was first observed in 2014 and 2015 on tomato plants in Israel and Jordan respectively. Since the first description, the virus has been reported from all continents except Oceania and Antarctica, and has been found infecting both tomato and pepper crops. In October 2019, the Dutch National Plant Protection Organization received a ToBRFV infected tomato sample as part of a generic survey targeting tomato pests. Presence of the virus was verified using Illumina sequencing. A follow-up survey was initiated to determine the extent of ToBRFV presence in the Dutch tomato horticulture and identify possible linkages between ToBRFV genotypes, companies and epidemiological traits. Nextstrain was used to visualize these potential connections. By November 2019, 68 companies had been visited of which 17 companies were found to be infected. The 50 ToBRFV genomes from these outbreak locations group in three main clusters, which are hypothesized to represent three original sources. No correlation was found between genotypes, companies and epidemiological traits, and the source(s) of the Dutch ToBRFV outbreak remain unknown. This paper describes a Nextstrain build containing ToBRFV genomes up to and including November 2019. Sharing data with this interactive online tool will enable the plant virology field to better understand and communicate the diversity and spread of this new virus. Organizations are invited to share data or materials for inclusion in the Nextstrain build, which can be accessed at https://nextstrain.nrcnvwa.nl/ ToBRFV/20191231.

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Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Real-time tracking of Tomato brown rugose fruit virus (ToBRFV) outbreaks in the Netherlands using Nextstrain

Vossenberg¹,

Visser²,

Bruinsma³

et al. 2020

PLoS ONE

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“…ToBRFV, in genus Tobamovirus and family Virgaviridae , was first identified in the Middle East ( 2 , 4 ). ToBRFV has since been reported in a number of countries in Asia ( 5 , 6 ), Europe ( 7 – 11 ), and North America ( 12 – 14 ) and is likely to spread to other countries. To curb virus spread, the U.S. Department of Agriculture issued a federal order in 2019 for ToBRFV ( 15 ).…”

Section: Announcementmentioning

confidence: 99%

Complete Genome Sequence of a Tomato Brown Rugose Fruit Virus Isolated in the United States

Chanda

Rivera

Nunziata

et al. 2020

Microbiol Resour Announc

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“…La muestra dio positivo para ToBRFV y PepMV, pero negativo para virus del mosaico del tomate, el resultado de ToBRFV fue confirmado por RT-PCR convencional con el empleo de cebadores específicos ToBRFV-F (5-AATGTCCATGTTTGTTACGCC-3) y ToBRFV-R (5-CGAATGTGATTTAAAACTGTGAAT-3) (Alkowni et al, 2019) y con cebadores genéricos de tobamovirus 514 Tombamo-s1 / 515 Tobamo-as1 (Menzel et al, 2019). La secuenciación de ToBRFV (MN182533) tenía entre 99,7 y 99,9 % de identidad con genomas de aislados de ToBRFV de Israel (KX619418.1), Jordania (KT383474.1), Alemania (MK133095.1, MK133093.1), México (MK319944.1) y Palestina (MK165457.1) (Skelton et al, 2019). En el Cuadro 2 se muestran los informes de la presencia del ToBRFV a partir de de su aparición en Israel y Jordania.…”

Section: Primer Reporte Y Propagación En El Mundounclassified

“…Posterior a la detección del virus de la fruta rugosa marrón del tomate (ToBRFV) en Israel y Jordania, la presencia de este virus se reportó en julio de 2018 en Westphalia, Alemania (Menzel et al, 2019), y Baja California, México (Camacho-Beltrán et al, 2019), en septiembre de 2018 en California, Estados Unidos (Ling et al, 2019) y en Michoacán, México (Cambrón-Crisantos et al, 2018); y en octubre de 2018 en Sicilia, Italia (Panno et al, 2019). Para el año de 2019 se reportó en Antalya, Turquía (Fidan et al, 2019); Shandong, China (Yan et al, 2019) y en sur de Inglaterra (Skelton et al, 2019).…”

Section: Introductionunclassified

Métodos de identificación del virus de la fruta rugosa marrón del tomate (ToBRFV) en México

et al. 2020

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Introducción. El virus de la fruta rugosa marrón del tomate (ToBRFV) se reportó por primera vez en Israel y Jordania en 2014, causa daños en cultivos de jitomate en agricultura protegida. Objetivo. Recopilar información de la distribución conocida, situación actual y métodos de detección del ToBRFV. Desarrollo. Entre 2018 y 2019 se confirmó la presencia del virus en México, Estados Unidos, China, Alemania, Turquía, Reino Unido e Italia. Para la detección del virus se ha empleado microscopía electrónica y pruebas serológicas, pero estas no pueden diferenciar el ToBRFV de otros tobamovirus, la técnica de diagnóstico más eficaz para la detección son oligonucleótidos específicos desarrollados a partir de los aislados de Israel y Jordania. Los protocolos de higiene son el paso más importante en la prevención de las infecciones. Conclusión. Los esfuerzos para controlar el ToBRFV se centran actualmente en el uso de estrictas prácticas de sanidad y detección en semilla y planta, mediante técnicas moleculares de reacción en cadena de polimerasa con transcriptasa inversa (RT-PCR) con oligonucleótidos específicos, para evitar falsos positivos.

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First report of Tomato brown rugose fruit virus in tomato in the United Kingdom

Cited by 60 publications

References 4 publications

Real-time tracking of Tomato brown rugose fruit virus (ToBRFV) outbreaks in the Netherlands using Nextstrain

Real-time tracking of Tomato brown rugose fruit virus (ToBRFV) outbreaks in the Netherlands using Nextstrain

Complete Genome Sequence of a Tomato Brown Rugose Fruit Virus Isolated in the United States

Métodos de identificación del virus de la fruta rugosa marrón del tomate (ToBRFV) en México

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