First report of <i>Tomato brown rugose fruit virus</i> on tomato in Turkey

Fidan, Hakan; Sarıkaya, Pelin; Çaliş, Özer

doi:10.5197/j.2044-0588.2019.039.018

Cited by 75 publications

(54 citation statements)

References 2 publications

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“…Tomato brown rugose fruit virus (ToBRFV) is a member of the Tobamovirus genus with a host range including tomato, pepper and some weeds such as nightshades [3]. It was first detected in Israel in 2014 [4] and later in countries in North America [5,6], Asia [7–9] and Europe [10,11]. Within North America, both the United States [12] and Mexico [13] consider ToBRFV to be a regulated pest.…”

Section: Introductionmentioning

confidence: 99%

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection ofTomato brown rugose fruit virus(ToBRFV)

Sarkes

Feindel

et al. 2020

Preprint

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Tomato brown rugose fruit virus (ToBRFV) is a member of Tobamovirus infecting tomato and pepper. Within North America, both the United States and Mexico consider ToBRFV to be a regulated pest. In Canada, the presence of ToBRFV has been reported, but an efficient diagnostic system has not yet been established. Here, we describe the development and assessment of a loop-mediated isothermal amplification (LAMP)-based assay to detect ToBRFV. The LAMP test was efficient and robust, and results could be obtained within 35 min with an available RNA sample. Amplification was possible when either water bath or oven were used to maintain the temperature at isothermal conditions (65°C), and results could be read by visual observation of colour change. Detection limit of the LAMP was eight target RNA molecules. Under the experimental conditions tested, LAMP was as sensitive as qPCR and 100 times more sensitive than the currently used rt-PCR. We recommend this sensitive, efficient LAMP protocol to be used for routine lab testing of ToBRFV.

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Section: Introductionmentioning

confidence: 99%

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection ofTomato brown rugose fruit virus(ToBRFV)

Sarkes

Feindel

et al. 2020

Preprint

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“…To date, the incidence and current distribution of ToBRFV infection have been reported from Jordan (Salem et al , ), Israel (Luria et al , ), Mexico (Cambrón‐Crisantos et al , ), the USA (Chitambar, ; Ling et al , ), Germany (Menzel et al , ), Italy (Panno et al , ), Palestine (Alkowni et al , ), Turkey (Fidan et al , ) and China (Yan et al , ). Likely occurrences have also been reported but not confirmed in Chile, Ethiopia, Sudan and the Netherlands.…”

Section: Emergence and Symptoms Of Tobrfv In Tomato Productionmentioning

confidence: 99%

Tomato brown rugose fruit disease: current distribution, knowledge and future prospects

et al. 2019

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Tomato is the most economically important fruit/vegetable crop grown worldwide. However, viral diseases remain an important factor limiting its productivity, with estimated quantitative and qualitative yield losses in tomato crops often reaching up to 100%. Many viruses infecting tomato have been reported, while new viral diseases have also emerged. The climatic changes the world is experiencing can be a contributing factor to the successful spread of newly emerging viruses, as well as the establishment of disease in areas that were previously either unfavourable or where the disease was absent. Because antiviral products are not available, strategies to mitigate viral diseases rely on genetic resistance/tolerance to infection, control of vectors, improvement in crop hygiene, roguing of infected plants and seed certification. Tomato brown rugose fruit virus (ToBRFV) is an emerging viral threat to tomato productivity and is currently spreading into new areas, which is of great concern to the growing global production in the absence of mitigation measures. This review presents the current knowledge about ToBRFV and future prospects for an improved understanding of the virus, which will be needed to support effective control and mitigation of the impact it is likely to cause.

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“…Tomato brown rugose fruit virus (ToBRFV) is a member of the Tobamovirus genus with a host range including tomato, pepper and some weeds such as nightshades [4]. It was first detected in Israel in 2014 [5] and later in countries in North America [6,7], Asia [8][9][10] and Europe [11,12]. Within North America, both the United States [13] and Mexico [14] consider ToBRFV to be a regulated pest.…”

Section: Introductionmentioning

confidence: 99%

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection of Tomato brown rugose fruit virus (ToBRFV)

Sarkes

Feindel

et al. 2020

PLoS ONE

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Tomato brown rugose fruit virus (ToBRFV) is a member of Tobamovirus infecting tomato and pepper. Within North America, both the United States and Mexico consider ToBRFV to be a regulated pest. In Canada, the presence of ToBRFV has been reported, but an efficient diagnostic system has not yet been established. Here, we describe the development and assessment of a loop-mediated isothermal amplification (LAMP)-based assay to detect ToBRFV. The LAMP test was efficient and robust, and results could be obtained within 35 min with an available RNA sample. Amplification was possible when either water bath or oven were used to maintain the temperature at isothermal conditions (65˚C), and results could be read by visual observation of colour change. Detection limit of the LAMP was eight target RNA molecules. Under the experimental conditions tested, LAMP was as sensitive as qPCR and 100 times more sensitive than the currently used RT-PCR. We recommend this sensitive, efficient LAMP protocol to be used for routine lab testing of ToBRFV.

show abstract

First report of Tomato brown rugose fruit virus on tomato in Turkey

Cited by 75 publications

References 2 publications

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection ofTomato brown rugose fruit virus(ToBRFV)

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection ofTomato brown rugose fruit virus(ToBRFV)

Tomato brown rugose fruit disease: current distribution, knowledge and future prospects

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection of Tomato brown rugose fruit virus (ToBRFV)

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