First Report of <i>Pestalotiopsis microspora</i> as a Causal Agent of Black Spot of Pecan (<i>Carya illinoinensis</i>) in China

Shi, Haoran; Zhang, C. Q.; Shan, Liuying; Xu, Kai; Xu, Jinsong; Qi, Q. Q.; Xu, Z.H.

doi:10.1094/pdis-01-15-0079-pdn

Cited by 10 publications

(10 citation statements)

References 3 publications

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“…46,47 However, previous studies mainly focus on isolation, purification, and identification of the pathogenic fungi of black spot disease, as well as prevention and control. For example, Shi et al 48 showed that the pathogen of black spot of Chaenomeles cathayensis was Pestalotiopsis microspora, and a pathogen of anthracnose (Colletotrichum nymphaeae) was isolated from the fruit of C. cathayensis for the first time. 49 For control of black spot, prochloraz and chlorpromazine are highly toxic to C. fioriniae 11 and therefore can be used in production to control the black spot of C. fioriniae caused by anthracnose.…”

Section: ■ Discussionmentioning

confidence: 99%

“…Genes are deterministic terms of phenotype, and transcriptome sequencing can provide great insights into the functions of pecan black spot disease genes. , However, previous studies mainly focus on isolation, purification, and identification of the pathogenic fungi of black spot disease, as well as prevention and control. For example, Shi et al showed that the pathogen of black spot of Chaenomeles cathayensis was Pestalotiopsis microspora, and a pathogen of anthracnose (Colletotrichum nymphaeae) was isolated from the fruit of C. cathayensis for the first time .…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome Analysis of Resistant and Susceptible Pecan (Carya illinoinensis) Reveals the Mechanism of Resistance to Black Spot Disease (Colletotrichum fioriniae)

Chang

Wang

Zhang

et al. 2023

J. Agric. Food Chem.

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Pecan, Carya illinoinensis (Wangenh.) K. Koch, is an important dried fruit and woody oil tree species grown worldwide. With continuous expansion of pecan cultivation, the frequency and scope of diseases, especially black spot disease, are increasing, damaging trees and reducing yields. In this study, the key factors in resistance to black spot disease (Colletotrichum fioriniae) were investigated between the high-resistance pecan variety “Kanza” and the low-resistance variety “Mahan”. Leaf anatomy and antioxidase activities confirmed much stronger resistance to black spot disease in “Kanza” than in “Mahan”. Transcriptome analysis indicated that the increased expression of genes associated with defense response, oxidation–reduction, and catalytic activity was involved in disease resistance. A connection network identified a highly expressed hub gene CiFSD2 (CIL1242S0042), which might participate in redox reactions to affect disease resistance. Overexpression of CiFSD2 in tobacco inhibited enlargement of necrotic spots and increased disease resistance. Overall, the expression of differentially expressed genes differed in pecan varieties with different levels of resistance to C. fioriniae infection. In addition, the hub genes associated with black spot resistance were identified and the functions clarified. The in-depth understanding of resistance to black spot disease provides new insights for early screening of resistant varieties and molecular-assisted breeding in pecan.

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Section: ■ Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Resistant and Susceptible Pecan (Carya illinoinensis) Reveals the Mechanism of Resistance to Black Spot Disease (Colletotrichum fioriniae)

Chang

Wang

Zhang

et al. 2023

J. Agric. Food Chem.

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“…It originated in North America and has been widely planted in China in recent years, including in Jiangsu and Anhui Provinces [ 1 ]. Fruit black spot (FBS) is a critical pathogen observed in pecan cultivation that impedes plant growth, limits overall crop productivity, and affects seed quality [ 2 ]. At present, reports on pecan– Pestalotiopsis microspora interaction mechanisms are limited.…”

Section: Introductionmentioning

confidence: 99%

“…The yield and quality of pecan are strongly affected by various biotic and abiotic stresses, such as salt [ 3 , 4 ], drought [ 5 ], and pathogens [ 6 ]. Among the wound pathogens, black spot is a fungal disease caused by Pestalotiopsis microspora , which leads to decayed pecan fruits [ 2 ]. In some other plants, such as citrus, this pathogen can produce mechanical pressure against host plant cells and can steal nutrients and water from infected plant tissues, and metabolites including enzymes (cutinase, protease, and cell wall hydrolase) and toxins (peptides, terpenoids, and others proteins) that are harmful to the normal physiological behavior of the host will be released [ 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis to Unravel Potential Pathways and Genes Involved in Pecan (Carya illinoinensis) Resistance to Pestalotiopsis microspora

Chen

Zhang

Zhao

et al. 2022

IJMS

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Fruit black spot (FBS), a fungal disease of pecan (Carya illinoinensis (Wangenh) K. Koch) caused by the pathogen Pestalotiopsis microspora, is a serious disease and poses a critical threat to pecan yield and quality. However, the details of pecan responses to FBS infection at the transcriptional level remain to be elucidated. In present study, we used RNA-Seq to analyze differential gene expression in three pecan cultivars with varied resistance to FBS infection: Xinxuan-4 (X4), Mahan (M), and Wichita (W), which were categorized as having low, mild, and high susceptibility to FBS, respectively. Nine RNA-Seq libraries were constructed, comprising a total of 58.56 Gb of high-quality bases, and 2420, 4380, and 8754 differentially expressed genes (DEGs) with |log2Fold change| ≥ 1 and p-value < 0.05 were identified between M vs. X4, W vs. M, and W vs. X4, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway analyses were performed to further annotate DEGs that were part of specific pathways, which revealed that out of 134 total pathways, MAPK signaling pathway, plant–pathogen interaction, and plant hormone signal transduction were highly enriched. Transcriptomic profiling analysis revealed that 1681 pathogen-related genes (PRGs), including 24 genes encoding WRKY transcription factors, potentially participate in the process of defense against Pestalotiopsis microspora infection in pecan. The correlation of WRKY TFs and PRGs was also performed to reveal the potential interaction networks among disease-resistance/pathogenesis-related genes and WRKY TFs. Expression profiling of nine genes annotated as TIFY, WRKY TF, and disease-resistance protein-related genes was performed using qRT-PCR, and the results were correlated with RNA-Seq data. This study provides valuable information on the molecular basis of pecan–Pestalotiopsis microspora interaction mechanisms and offers a repertoire of candidate genes related to pecan fruit response to FBS infection.

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“…Despite its great harmless to pine wood, the genetic information of M. alternatus has remained largely unknown, the genome size and genome-wide sequencing of M. alternatus have not been reported. The mitogenomes of M. alternatus, Bursaphelenchus mucronatus, and some of the closely related species have been studied [12]. Formerly, scholars have used morphology, anatomy and physiology, and other methods to establish relationships among species for creating classification systems.…”

Section: Introductionmentioning

confidence: 99%

Genome Size Survey of the Japanese Pine Sawyer Beetle, Monochamus alternatus (Coleoptera: Cerambycidae)

Fan

Fang

et al. 2022

CJAST

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Monochamus alternatus is a longicorn beetle, an important stem borer of pine trees in China. In this study, we determined the first accurate genomic survey of M. alternatus by the next-generation sequencing (NGS), which is a relatively new methodology that can ensure the identification of large numbers of simple sequences repeat (SSR) markers, in order to increases the abundance of the Cerambycidae genome information and assist in phylogenetic, molecular systematics and evolutionary studies for Coleoptera. The result showed that the genome size was 871.09 Mb, the GC content of the genome was within normal limits (34.45%), the proportion of repetitive sequences was high (59.40%), and the heterozygosity rate was low (1.04%). The heterozygosity of M. alternatus is higher than 0.5% and the repeat rate is more than 50%. Based on this we inferred the use of Illumina+PacBio in sequencing assembly strategy is highly recommended.

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First Report of Pestalotiopsis microspora as a Causal Agent of Black Spot of Pecan (Carya illinoinensis) in China

Cited by 10 publications

References 3 publications

Transcriptome Analysis of Resistant and Susceptible Pecan (Carya illinoinensis) Reveals the Mechanism of Resistance to Black Spot Disease (Colletotrichum fioriniae)

Transcriptome Analysis of Resistant and Susceptible Pecan (Carya illinoinensis) Reveals the Mechanism of Resistance to Black Spot Disease (Colletotrichum fioriniae)

Transcriptomic Analysis to Unravel Potential Pathways and Genes Involved in Pecan (Carya illinoinensis) Resistance to Pestalotiopsis microspora

Genome Size Survey of the Japanese Pine Sawyer Beetle, Monochamus alternatus (Coleoptera: Cerambycidae)

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