First Report of <i>Pectobacterium parmentieri</i> Causing Stem Rot Disease of Potato in Russia

Ha, Vo Thi Ngoc; Воронина, М. В.; Kabanova, Anastasia P.; Shneider, Mikhail M.; Korzhenkov, Aleksei A.; Toschakov, Stepan V; Miroshnikov, Kirill K.; Miroshnikov, Konstantin A.; Игнатов, А. Н.

doi:10.1094/pdis-11-17-1829-pdn

Cited by 6 publications

(2 citation statements)

References 3 publications

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“…No representatives of Dickeya spp. and P. atrosepticum (Pat), which resulted in severe outbreaks of soft rot in the 2000s, and P. parmentieri (Ppa), which recently emerged as an abundant potato pathogen [24,25], were detected. The diversity of the isolated species was more correlated to the cultivar and seed lot of potato, rather than to geographic location or the particular weather conditions of the growing season.…”

Section: Phytopathogens Causing Soft Rot Of Potato In Northern Moscow Region In 2019mentioning

confidence: 99%

Use of a Specific Phage Cocktail for Soft Rot Control on Ware Potatoes: A Case Study

Bugaeva

Воронина²,

Vasiliev³

et al. 2021

Viruses

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Using bacteriophages (bacterial viruses) to control pathogenic bacteria is a promising approach in horticulture. However, the application of this strategy in real conditions requires compliance with particular technological and environmental restraints. The presented paper concerns the process of phage selection to create a cocktail that is efficient against the circulating causal agents of potato soft rot. The resulting phage cocktail causes a complete lysis of a mixture of circulating pectobacterial strains in vitro. In the context of being used to treat ware potatoes during off-season storage, the protocol of phage application via the humidity maintenance system was designed. The phage cocktail was shown to reduce the population of Pectobacterium spp. 10–12-fold, achieving a population that was below a symptomatic threshold.

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Section: Phytopathogens Causing Soft Rot Of Potato In Northern Moscow Region In 2019mentioning

confidence: 99%

Use of a Specific Phage Cocktail for Soft Rot Control on Ware Potatoes: A Case Study

Bugaeva

Воронина²,

Vasiliev³

et al. 2021

Viruses

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“…Pectobacterium parmentieri , previously known as P. wasabiae, a gram-negative, rod-shaped virulent pectolytic pathogenic bacterium 7 , commonly isolated from potato plants and tubers displaying blackleg and soft rot symptoms, is able to survive under a range of environmental conditions. Latently infected potato seed-tuber and contaminated propagative plant materials contribute to the dispersal of P. parmentieri; pathogen has been identified in several regions of Europe 8 , Canada 9 , United States 10 , New Zealand, China 11 and South Africa 12 .…”

Section: Introductionmentioning

confidence: 99%

Genome-informed loop-mediated isothermal amplification assay for specific detection of Pectobacterium parmentieri in infected potato tissues and soil

Domingo

Pérez

Klair

et al. 2021

Sci Rep

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Pectobacterium parmentieri (formerly Pectobacterium wasabiae), which causes soft rot disease in potatoes, is a newly established species of pectinolytic bacteria within the family Pectobacteriaceae. Despite serious damage caused to the potato industry worldwide, no field-deployable diagnostic tests are available to detect the pathogen in plant samples. In this study, we aimed to develop a reliable, rapid, field-deployable loop-mediated isothermal amplification (LAMP) assay for the specific detection of P. parmentieri. Specific LAMP primers targeting the petF1 gene region, found in P. parmentieri but no other Pectobacterium spp., were designed and validated in silico and in vitro using extensive inclusivity (15 strains of P. parmentieri) and exclusivity (94 strains including all other species in the genus Pectobacterium and host DNA) panels. No false positives or negatives were detected when the assay was tested directly with bacterial colonies, and with infected plant and soil samples. Sensitivity (analytical) assays using serially diluted bacterial cell lysate and purified genomic DNA established the detection limit at 10 CFU/mL and 100 fg (18–20 genome copies), respectively, even in the presence of host crude DNA. Consistent results obtained by multiple users/operators and field tests suggest the assay’s applicability to routine diagnostics, seed certification programs, biosecurity, and epidemiological studies.

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Diseases Caused by Pectobacterium and Dickeya Species Around the World

Wolf

Acuña

Boer

et al. 2021

Plant Diseases Caused by Dickeya and Pectobacterium Species

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Article 25fa states that the author of a short scientific work funded either wholly or partially by Dutch public funds is entitled to make that work publicly available for no consideration following a reasonable period of time after the work was first published, provided that clear reference is made to the source of the first publication of the work.This publication is distributed under The Association of Universities in the Netherlands (VSNU) 'Article 25fa implementation' project. In this project research outputs of researchers employed by Dutch Universities that comply with the legal requirements of Article 25fa of the Dutch Copyright Act are distributed online and free of cost or other barriers in institutional repositories. Research outputs are distributed six months after their first online publication in the original published version and with proper attribution to the source of the original publication.

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First Report of Pectobacterium parmentieri Causing Stem Rot Disease of Potato in Russia

Cited by 6 publications

References 3 publications

Use of a Specific Phage Cocktail for Soft Rot Control on Ware Potatoes: A Case Study

Use of a Specific Phage Cocktail for Soft Rot Control on Ware Potatoes: A Case Study

Genome-informed loop-mediated isothermal amplification assay for specific detection of Pectobacterium parmentieri in infected potato tissues and soil

Diseases Caused by Pectobacterium and Dickeya Species Around the World

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