Use of a Specific Phage Cocktail for Soft Rot Control on Ware Potatoes: A Case Study

Bugaeva, Eugenia N; Воронина, М. В.; Vasiliev, Dmitry M; Lukianova, Anna A.; Landyshev, Nikolay N.; Игнатов, А. Н.; Miroshnikov, Konstantin A.

doi:10.3390/v13061095

Cited by 10 publications

(6 citation statements)

References 32 publications

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“…Even the taxons established long ago (e.g., P. atrosepticum within SRP) may contain “orphan” strain groups with phage susceptibility different from most other representatives of this species. In the case of P. versatile , the strains similar to F131 are the examples of such “orphan” strains, which are, nevertheless, abundant among SRPs circulating in Russia [ 72 ]. Therefore, the phages like Possum infecting such strains should be included to the prospective cocktails for phage control of SRP despite a narrow host range.…”

Section: Discussionmentioning

confidence: 99%

Pectobacterium versatile Bacteriophage Possum: A Complex Polysaccharide-Deacetylating Tail Fiber as a Tool for Host Recognition in Pectobacterial Schitoviridae

Lukianova

Evseev

Shneider

et al. 2022

IJMS

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Novel, closely related phages Possum and Horatius infect Pectobacterium versatile, a phytopathogen causing soft rot in potatoes and other essential plants. Their properties and genomic composition define them as N4-like bacteriophages of the genus Cbunavirus, a part of a recently formed family Schitoviridae. It is proposed that the adsorption apparatus of these phages consists of tail fibers connected to the virion through an adapter protein. Tail fibers possess an enzymatic domain. Phage Possum uses it to deacetylate O-polysaccharide on the surface of the host strain to provide viral attachment. Such an infection mechanism is supposed to be common for all Cbunavirus phages and this feature should be considered when designing cocktails for phage control of soft rot.

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Section: Discussionmentioning

confidence: 99%

Pectobacterium versatile Bacteriophage Possum: A Complex Polysaccharide-Deacetylating Tail Fiber as a Tool for Host Recognition in Pectobacterial Schitoviridae

Lukianova

Evseev

Shneider

et al. 2022

IJMS

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“…This work reports that, starting from almost negligible values, the population of Ppa grew fast at room temperature and reached ~10 6 cfu/mL, resulting in tissue rotting in a few days. On the other hand, undamaged potato tubers with a latent SRP population 10 4– 10 6 cfu/mL on the skin revealed no signs of soft rot being stored in proper warehouse conditions (4–7 °C) [ 40 ]. Therefore, the monitoring of the bacterial insemination of the tubers may help to estimate the risk of soft rot development in the stored tubers and to reveal the dangerous threshold for each particular SRP species.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Real-Time PCR Assay for the Detection of Pectobacterium parmentieri, a Causal Agent of Potato Soft Rot

Lukianova

Evseev

Stakheev

et al. 2021

Plants

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Pectobacterium parmentieri is a plant-pathogenic bacterium, recently attributed as a separate species, which infects potatoes, causing soft rot in tubers. The distribution of P. parmentieri seems to be global, although the bacterium tends to be accommodated to moderate climates. Fast and accurate detection systems for this pathogen are needed to study its biology and to identify latent infection in potatoes and other plant hosts. The current paper reports on the development of a specific and sensitive detection protocol based on a real-time PCR with a TaqMan probe for P. parmentieri, and its evaluation. In sensitivity assays, the detection threshold of this protocol was 102 cfu/mL on pure bacterial cultures and 102–103 cfu/mL on plant material. The specificity of the protocol was evaluated against P. parmentieri and more than 100 strains of potato-associated species of Pectobacterium and Dickeya. No cross-reaction with the non-target bacterial species, or loss of sensitivity, was observed. This specific and sensitive diagnostic tool may reveal a wider distribution and host range for P. parmentieri and will expand knowledge of the life cycle and environmental preferences of this pathogen.

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“…carotovorum and Dickeya spp. [60,[82][83][84][85]. Potato common scab (PCS) refers to soil-borne diseases that do not respond well to chemical control and require treatments that disrupt the integrity of the ecosystem [86].…”

Section: Impact Of Bacterial and Fungal Pathogens On Potato Microbiomementioning

confidence: 99%

Potato Microbiome: Relationship with Environmental Factors and Approaches for Microbiome Modulation

Petrushin,

Filinova,

Gutnik

2024

IJMS

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Every land plant exists in a close relationship with microbial communities of several niches: rhizosphere, endosphere, phyllosphere, etc. The growth and yield of potato—a critical food crop worldwide—highly depend on the diversity and structure of the bacterial and fungal communities with which the potato plant coexists. The potato plant has a specific part, tubers, and the soil near the tubers as a sub-compartment is usually called the “geocaulosphere”, which is associated with the storage process and tare soil microbiome. Specific microbes can help the plant to adapt to particular environmental conditions and resist pathogens. There are a number of approaches to modulate the microbiome that provide organisms with desired features during inoculation. The mechanisms of plant–bacterial communication remain understudied, and for further engineering of microbiomes with particular features, the knowledge on the potato microbiome should be summarized. The most recent approaches to microbiome engineering include the construction of a synthetic microbial community or management of the plant microbiome using genome engineering. In this review, the various factors that determine the microbiome of potato and approaches that allow us to mitigate the negative impact of drought and pathogens are surveyed.

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Use of a Specific Phage Cocktail for Soft Rot Control on Ware Potatoes: A Case Study

Cited by 10 publications

References 32 publications

Pectobacterium versatile Bacteriophage Possum: A Complex Polysaccharide-Deacetylating Tail Fiber as a Tool for Host Recognition in Pectobacterial Schitoviridae

Pectobacterium versatile Bacteriophage Possum: A Complex Polysaccharide-Deacetylating Tail Fiber as a Tool for Host Recognition in Pectobacterial Schitoviridae

Quantitative Real-Time PCR Assay for the Detection of Pectobacterium parmentieri, a Causal Agent of Potato Soft Rot

Potato Microbiome: Relationship with Environmental Factors and Approaches for Microbiome Modulation

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