First Report of Pectobacterium aroidearum and Pectobacterium carotovorum subsp. brasiliensis Causing Soft Rot of Cucurbita pepo in Brazil

Moraes, Alessandra Jackeline Guedes de; Souza, Elineide Barbosa de; Mariano, R. L. R.; Silva, Adriano Márcio Freire; Lima, Nelson; Peixoto, Ana Rosa; Gama, Marco Aurélio Siqueira da

doi:10.1094/pdis-08-16-1168-pdn

Cited by 27 publications

(17 citation statements)

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“…Of these, Pectobacterium carotovorum subsp. brasiliense is an emerging SRE with global distribution (van der Merwe et al, 2010; Panda et al, 2012; Onkendi and Moleleki, 2014; Moraes et al, 2017; Ma et al, 2018; Voronina et al, 2019). Previous studies have shown that Pcb strain PBR1692 (referred to henceforth as Pcb 1692) is able to inhibit growth of Pectobacterium carotovorum subsp.…”

Section: Introductionmentioning

confidence: 99%

The Impact of Type VI Secretion System, Bacteriocins and Antibiotics on Bacterial Competition of Pectobacterium carotovorum subsp. brasiliense and the Regulation of Carbapenem Biosynthesis by Iron and the Ferric-Uptake Regulator

et al. 2019

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The complexity of plant microbial communities provides a rich model for investigating biochemical and regulatory strategies involved in interbacterial competition. Within these niches, the soft rot Enterobacteriaceae (SRE) represents an emerging group of plant–pathogens causing soft rot/blackleg diseases resulting in economic losses worldwide in a variety of crops. A preliminary screening using next-generation sequencing of 16S rRNA comparatively analyzing healthy and diseased potato tubers, identified several taxa from Proteobacteria to Firmicutes as potential potato endophytes/plant pathogens. Subsequent to this, a range of molecular and computational techniques were used to determine the contribution of antimicrobial factors such as bacteriocins, carbapenem and type VI secretion system (T6SS), found in an aggressive SRE (Pectobacterium carotovorum subsp. brasiliense strain PBR1692 – Pcb1692) against these endophytes/plant pathogens. The results showed growth inhibition of several Proteobacteria by Pcb1692 depends either on carbapenem or pyocin production. Whereas for targeted Firmicutes, only the Pcb1692 pyocin seems to play a role in growth inhibition. Furthermore, production of carbapenem by Pcb1692 was observably dependent on the presence of environmental iron and oxygen. Additionally, upon deletion of fur, slyA and expI regulators, carbapenem production ceased, implying a complex regulatory mechanism involving these three genes. Finally, the results demonstrated that although T6SS confers no relevant advantage during in vitro competition, a significant attenuation in competition by the mutant strain lacking a functional T6SS was observed in planta.IMPORTANCESoft rot Enterobacteriaceae (SRE) represents important phytopathogens causing soft rot/blackleg diseases in a variety of crops leading to huge economic losses worldwide. These pathogens have been isolated alongside other bacteria from different environments such as potato tubers, stems, roots and from the soil. In these environments, SREs coexist with other bacteria where they have to compete for scarce nutrients and other resources. In this report, we show that Pectobacterium carotovorum subsp. brasiliense strain PBR1692 – Pcb1692, which represents one of the SREs, inhibits growth of several different bacteria by producing different antimicrobial compounds. These antimicrobial compounds can be secreted inside or outside the plant host, allowing Pcb1692 to effectively colonize different types of ecological niches. By analyzing the genome sequences of several SREs, we show that other SREs likely deploy similar antimicrobials to target other bacteria.

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Section: Introductionmentioning

confidence: 99%

The Impact of Type VI Secretion System, Bacteriocins and Antibiotics on Bacterial Competition of Pectobacterium carotovorum subsp. brasiliense and the Regulation of Carbapenem Biosynthesis by Iron and the Ferric-Uptake Regulator

et al. 2019

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“…P. aroidearum has been described as a plant pathogen with a preference for monocotyledonous plants (Nabhan et al 2013). However, in recent years it has been observed causing soft rot in different dicotyledonous plants, such as zucchini (Moraes et al 2017), lettuce, Chinese cabbage (Barroso et al 2019) and, in the present study, pepper fruits. Thus, to our knowledge, this is the first report of P. aroidearum causing soft rot in pepper fruits in Brazil.…”

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confidence: 46%

First Report of Pectobacterium aroidearum Causing Soft Rot of Pepper (Capsicum annuum) Fruits in Brazil

et al. 2020

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“…'aroideae' probably correspond to Pectobacterium carotovorum subsp. carotovorum and not Pectobacterium aroidearum, which is primarily a pathogen of monocotyledonous hosts (Nabhan et al 2013) and most recently has been detected from potatoes (Moretti et al 2016) and zucchini (Moraes et al 2017). However, its presence has not been confirmed in phylogenetic analysis (data not shown).…”

Section: Discussionmentioning

confidence: 94%

Pectobacterium and Dickeya species detected in vegetables in Northern Ireland

et al. 2019

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Destructive soft rot Pectobacteriaceae affect a number of vegetable crops and cause high economic loses in the field and storage. The diversity of Pectobacterium and Dickeya causing soft rot of vegetables in Northern Ireland is unknown. This study provides details of Pectobacterium and Dickeya spp. detected in vegetables from several locations in Northern I r e l a n d i n t h e y e a r s 2 0 1 5-2 0 1 7. S o f t r o t Pectobacteriaceae were identified based on DNA sequences. Thirty three strains were selected for further phylogenetic analysis based on the recA gene region. Results from the testing of over 3456 potato samples for plant health statutory purposes in the years 2005-2017 demonstrated that Dickeya spp. is not the major pathogen causing soft rot or blackleg in Northern Ireland. The most predominant species causing soft rot of vegetables in Northern Ireland were Pectobacterium atrosepticum and Pectobacterium carotovorum subsp. carotovorum. Pectobacterium atrosepticum was also detected on hosts other than potato. Testing of bacteria isolated from carrots led to the detection of P. carotovorum and Dickeya sp. This is the first study to provide knowledge about Pectobacterium and Dickeya spp. diversity causing soft rot of vegetables in Northern Ireland confirmed by real-time PCR and DNA sequences. This is also the first report of the detection of D. aquatica from a source other than water.

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First Report of Pectobacterium aroidearum and Pectobacterium carotovorum subsp. brasiliensis Causing Soft Rot of Cucurbita pepo in Brazil

Cited by 27 publications

References 2 publications

The Impact of Type VI Secretion System, Bacteriocins and Antibiotics on Bacterial Competition of Pectobacterium carotovorum subsp. brasiliense and the Regulation of Carbapenem Biosynthesis by Iron and the Ferric-Uptake Regulator

The Impact of Type VI Secretion System, Bacteriocins and Antibiotics on Bacterial Competition of Pectobacterium carotovorum subsp. brasiliense and the Regulation of Carbapenem Biosynthesis by Iron and the Ferric-Uptake Regulator

First Report of Pectobacterium aroidearum Causing Soft Rot of Pepper (Capsicum annuum) Fruits in Brazil

Pectobacterium and Dickeya species detected in vegetables in Northern Ireland

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