Pectobacterium and Dickeya species detected in vegetables in Northern Ireland

Abstract

Destructive soft rot Pectobacteriaceae affect a number of vegetable crops and cause high economic loses in the field and storage. The diversity of Pectobacterium and Dickeya causing soft rot of vegetables in Northern Ireland is unknown. This study provides details of Pectobacterium and Dickeya spp. detected in vegetables from several locations in Northern I r e l a n d i n t h e y e a r s 2 0 1 5-2 0 1 7. S o f t r o t Pectobacteriaceae were identified based on DNA sequences. Thirty three strains were selected for further phylogenetic analysis based on the recA gene region. Results from the testing of over 3456 potato samples for plant health statutory purposes in the years 2005-2017 demonstrated that Dickeya spp. is not the major pathogen causing soft rot or blackleg in Northern Ireland. The most predominant species causing soft rot of vegetables in Northern Ireland were Pectobacterium atrosepticum and Pectobacterium carotovorum subsp. carotovorum. Pectobacterium atrosepticum was also detected on hosts other than potato. Testing of bacteria isolated from carrots led to the detection of P. carotovorum and Dickeya sp. This is the first study to provide knowledge about Pectobacterium and Dickeya spp. diversity causing soft rot of vegetables in Northern Ireland confirmed by real-time PCR and DNA sequences. This is also the first report of the detection of D. aquatica from a source other than water.

Comparative genomics and pangenome-oriented studies reveal high homogeneity of the agronomically relevant enterobacterial plant pathogen Dickeya solani

“…The presence of D. solani strains was reported in Europe and beyond, e.g. in the Netherlands [19], Belgium [40], Israel [35], Turkey [41], Finland [28], Norway [42], Portugal [31], Czech Republic [43], Denmark [43], United Kingdom [44], Northern Ireland [45], Greece [46], France [47], Switzerland [48], Spain [49], Slovenia [50], Georgia [51], Russia [52], Germany [32], Brazil [53] and China [54]. Notably, the tested isolates originated from a limited number of plants including potato [27,28,35], hyacinth [23] and iris [19], which might be related to previous assumptions on strict linkage between highly specialized pathogens of clonal origin and their host [19,55].…”

DEVELOPMENT OF NEW qPCR-BASED IDENTIFICATION SYSTEMS FOR NON-QUARANTINE POTATO (Solanum tuberosum L.) PATHO-GENS DISTRIBUTED IN THE TERRITORY OF RUSSIA

“…(минимальная чувствительность -0,01 нг/мкл специфической ДНК) (36), однако они не были адаптированы в формат количественной ПЦР. Праймеры, позволяющие специфично различать виды рода Dickeya, были описаны в 2013 году (37) и успешно использованы при выявлении патогенов в овощных культурах на территории Северной Ирландии (38). Для такой тест-системы показана очень высокая чувствительность, составившая около 0,05 пг ДНК на реакцию (около 10 копий плазмидной ДНК).…”

РАЗРАБОТКА НОВЫХ СИСТЕМ ПЦР-ИДЕНТИФИКАЦИИ НЕКАРАНТИННЫХ ПАТОГЕНОВ КАРТОФЕЛЯ (Solanum tuberosum L.), РАСПРОСТРАНЕННЫХ НА ТЕРРИТОРИИ РОССИИ

“…(минимальная чувствительность -0,01 нг/мкл специфической ДНК) (36), однако они не были адаптированы в формат количественной ПЦР. Праймеры, позволяющие специфично различать виды рода Dickeya, были описаны в 2013 году (37) и успешно использованы при выявлении патогенов в овощных культурах на территории Северной Ирландии (38). Для такой тест-системы показана очень высокая чувствительность, составившая около 0,05 пг ДНК на реакцию (около 10 копий плазмидной ДНК).…”