First report of <i>Lasiodiplodia pseudotheobromae</i> causing fruit rot of persimmon in Brazil

Júnior, Antonio Fernandes Nogueira; Santos, R. F.; Pagenotto, Ana Carolina Viviani; Spósito, Marcel Bellato

doi:10.5197/j.2044-0588.2017.036.001

Cited by 12 publications

(7 citation statements)

References 3 publications

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“…Lasiodiplodia pseudotheobromae is better known as a common fungal pathogen that causes diseases on large number of hosts plants in the tropics and subtropics such as on mango which causes dieback disease [17,18], which can cause trunk cankers in Acacia mangium in Venezuela [19], postharvest fruit rot of lemon in Turkey [20], and persimmon rot in Brazil [21]. However, recently, it has been known as an endophytic fungus which residing in various parts of host plants and has been reported to exhibit various pharmaceutical activities such antibacterial [22,23], xanthine oxidase inhibitory [24], fibrinolytic [25], and anticancer [26] activities.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity of an endophytic fungus Lasiodiplodia pseudotheobromae IBRL OS-64 residing in leaves of a medicinal herb, Ocimum sanctum Linn

2019

jabb

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The study was carried out to investigate the antibacterial activity of an endophytic fungal isolate, Lasiodiplodia pseudotheobromae IBRL OS-64 residing in leaves of a medicinal herb, Ocimum sanctum Linn. Qualitative screening of the antimicrobial activity was done using an agar plug assay, and the results showed that the fungal isolate was able to inhibit all the 13 test bacteria. Three Gram-positive bacteria (methicillin-resistant Staphylococcus aureus [MRSA] ATCC 33591, Staphylococcus aureus, and Streptococcus mutans) were the most susceptible species with the inhibition zones of ≥21 mm. The other three (Bacillus cereus ATCC 10876, Bacillus subtilis IBRL A3, and Streptococcus agalactiae) showed the inhibition zones of 11-≤20 mm of diameter. As for Gram-negative bacteria, Yersinia enterocolitica was the most susceptible to the fungal isolate with the size of inhibition zone of ≥21 mm, followed by Klebsiella pneumoniae ATCC 13883, Salmonella typhimurium, and Shigella boydii ATCC 9207 with the inhibition zones of 11-≤20 mm, whereas Escherichia coli IBRL 0157, Proteus mirabilis, and Pseudomonas aeruginosa ATCC 27883 were the least susceptible with the inhibition zones of ≤10 mm. Quantitative screening using disc diffusion assay showed that the fungal ethyl acetate extract prepared from the fermentative broth (extracellular) exhibited better antibacterial activity compared to the methanolic extract prepared from the fungal biomass (intracellular). The results showed that the ethyl acetate extract exhibited antibacterial activity against all the 13 test bacteria with the inhibition zone sizes of 20.0 ± 0.3-31.3 ± 1.2 mm in diameter for Gram-positive bacteria and 10.31 ± 0.6-20.1 ± 0.6 mm in diameter for Gram-negative bacteria. On the other hand, the methanolic extract only inhibited three Gram-positive bacteria (MRSA ATCC 33591, S. aureus, and S. mutans) with the inhibition zones of 9.0 ± 0.6-11.0 ± 0.3 mm in diameter, whereas only one Gram-negative (S. typhimurium) with the inhibition zone size of 13.3 ± 1.5 mm diameter. The minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of the ethyl acetate extract on Gram-positive bacteria were in the range of 62.50-125.00 and 62.50-500.00 µg/mL, respectively, whereas for the Gram-negative bacteria, the MIC and MBC values were 125.00-250.00 and 250.0-1000.00 µg/mL, respectively. On the other hand, the MIC and MBC values for methanolic extract against Gram-positive bacteria were 250.00-500.00 µg/mL and against Gram-negative bacteria were 1000.00 µg/mL, respectively. Both of the extracts exhibited bactericidal effects on test bacteria with the MBC/MIC ratio ≤4. Further, detail of the effects of the ethyl acetate extract on the bacterial cells was observed from the scanning electron microscopy photomicrographs which revealed the severity of the morphological deterioration experienced by the extract-treated cells were beyond repair, and the most possible mode of actions were by interrupting the cell wall biosynthesis and cell membrane permea...

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Section: Introductionmentioning

confidence: 99%

Antibacterial activity of an endophytic fungus Lasiodiplodia pseudotheobromae IBRL OS-64 residing in leaves of a medicinal herb, Ocimum sanctum Linn

2019

jabb

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“…Lasiodiplodia pseudotheobromae is known to be one of the main causes of post-harvest fruit rot in longan fruits in Thailand (Pipattanapuckdee et al, 2019) and damaging persimmons in Brazil before and after harvest (Nogueira J unior et al, 2017). It also causes post-harvest rot in Citrus sp.…”

Section: Reported Evidence Of Impactmentioning

confidence: 99%

Commodity risk assessment of Juglans regia plants from Turkey

Bragard¹,

Dehnen‐Schmutz²,

Serio³

et al. 2021

EFS2

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The European Commission requested the EFSA Panel on Plant Health to prepare and deliver risk assessments for commodities listed in Commission Implementing Regulation (EU) 2018/2019 as 'High risk plants, plant products and other objects'. This Scientific Opinion covers the plant health risks posed by 2-year-old grafted bare rooted plants for planting of Juglans regia imported from Turkey, taking into account the available scientific information, including the technical information provided by Turkey. The relevance of any pest for this Opinion was based on evidence following defined criteria. Two EU quarantine pests, Anoplophora chinensis and Lopholeucaspis japonica, and three pests not regulated in the EU, two insects (Garella musculana, Euzophera semifuneralis) and one fungus (Lasiodiplodia pseudotheobromae), fulfilled all relevant criteria and were selected for further evaluation. For these pests, the risk mitigation measures proposed in the technical dossier from Turkey were evaluated by considering the possible limiting factors. For these pests, an expert judgement was given on the likelihood of pest freedom taking into consideration the risk mitigation measures acting on the pests, including uncertainties associated with the assessment. While the estimated degree of pest freedom varied among pests, Lasiodiplodia pseudotheobromae was the pest most frequently expected on the commodity. The expert knowledge elicitation indicated, with 95% certainty, that 9,554 or more grafted bare rooted plants per 10,000 will be free from Lasiodiplodia pseudotheobromae.

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“…Botryosphaeria dothidea (A14) was isolated from avocado and characterized by Firmino et al (2016). Lasiodiplodia pseudotheobromae (C1) was isolated and characterized for the first time from persimmon by Nogueira Júnior et al (2017). Neofusicoccum parvum (N10) was isolated from guava and previously characterized by Nogueira .…”

Section: Inoculation Procedures and Disease Evaluationmentioning

confidence: 99%

“…Fruit rot caused by Botryosphaeriaceae species has been reported in field and postharvest such as the rot of pitaya (Valencia et al, 2005), jackfruit (Ni et al, 2008), mango (Marques et al, 2013), avocado (Eskalen & McDonald, 2011;Feijo et al, 2019;Ni et al, 2009;Valencia et al, 2019), guava (Cedeno et al, 1998;Nogueira Júnior et al, 2016) and persimmon (Nogueira Júnior et al, 2017;Palou et al, 2013). Symptoms caused by Botryosphaeriaceae species on fruit can be observed in the peduncle (stem end) and in the area surrounding the fruit calyx (stylar end).…”

Section: Introductionmentioning

confidence: 99%

Penetration by Botryosphaeriaceae species in avocado, guava and persimmon fruit during postharvest

Navarro

Molina

Júnior

2021

Journal of Phytopathology

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Botryosphaeriaceae species have a wide host range and a worldwide distribution.These fungal species can colonize several plant organs, such as the trunk, leaves and fruit. Some Botryosphaeriaceae species cause important diseases on persimmon, avocado and guava fruit. However, there is a lack of information regarding the mechanisms of penetration by Botryosphaeriaceae species on these tropical and subtropical fruits.This study aimed to better understand the mechanisms involved in fungal penetration, host specificity and aggressiveness of Botryosphaeria dothidea, Lasiodiplodia pseudotheobromae and Neofusicoccum parvum on avocado (Persea americana), guava (Psidium guajava) and persimmon (Diospyros kaki) fruit. Scanning electron microscopy (SEM) image analysis showed that in avocado fruit, the three studied Botryosphaeriaceae species penetrated through lenticels. In guava fruit, penetration through stomata was verified for Botryosphaeria dothidea and Neofusicoccum parvum. In persimmon fruit, an appressoria-like structure was observed for B. dothidea, which suggests direct penetration. Disease incidence in wounded fruit was 24% higher than in non-wounded fruit. L. pseudotheobromae and N. parvum showed differences in aggressiveness in guava fruit. The longest incubation period was observed for N. parvum inoculated on guava, with an average of 4.5 days, and the shortest incubation period was verified for B. dothidea inoculated on avocado, with an average of 2.8 days. The area under the disease progress curve (AUDPC) did not differ between Botryosphaeriaceae species on avocado, whereas on guava and persimmon fruit, the AUDPC was lower for B. dothidea. The information regarding penetration mechanisms and aggressiveness is important to improve postharvest disease control strategies.

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First report of Lasiodiplodia pseudotheobromae causing fruit rot of persimmon in Brazil

Cited by 12 publications

References 3 publications

Antibacterial activity of an endophytic fungus Lasiodiplodia pseudotheobromae IBRL OS-64 residing in leaves of a medicinal herb, Ocimum sanctum Linn

Antibacterial activity of an endophytic fungus Lasiodiplodia pseudotheobromae IBRL OS-64 residing in leaves of a medicinal herb, Ocimum sanctum Linn

Commodity risk assessment of Juglans regia plants from Turkey

Penetration by Botryosphaeriaceae species in avocado, guava and persimmon fruit during postharvest

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