First Report of <i>Fusarium oxysporum</i> Isolate gx3 Causing Sugarcane Pokkah Boeng in Guangxi of China

Bao, Yixue; Sun, H. J.; Li, Y. F.; Duan, Zhenzhen; McCord, Per; Cui, Yuting; Zhang, M. Q.

doi:10.1094/pdis-01-16-0080-pdn

Cited by 12 publications

(8 citation statements)

References 4 publications

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“…The seriously damaged crops reported at home and abroad are eggplant, sugarcane, blackberry, cotton, etc. [ 43 , 44 , 45 , 46 ]. At present, chemical control is also the most common method to control plant diseases.…”

Section: Discussionmentioning

confidence: 99%

Detection and Control of Fusarium oxysporum from Soft Rot in Dendrobium officinale by Loop-Mediated Isothermal Amplification Assays

Xiao

2021

Biology

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Soft rot causing Fusarium oxysporum is one of the most destructive diseases of Dendrobium officinale Kimura et Migo in China that reduces D. officinale yield and quality. A key challenge for an integrated management strategy for this disease is the rapid and accurate detection of F. oxysporum on D. officinale. Therefore, a new loop-mediated isothermal amplification (LAMP) assay was developed for this purpose. In this study, the primers were selected and designed using the translation elongation factor-1α (TEF-1α) gene region as the target DNA sequence in order to screen the best system of reaction of LAMP to detect F. oxysporum through optimizing different conditions of the LAMP reaction, including time, temperature, concentrations of MgSO4, and concentrations of inner and outer primers. The optimized system was able to efficiently amplify the target gene at 62 °C for 60 min with 1.2 μM internal primers, 0.4 μM external primers, 7 mM Mg2+, and 5 fg/µL minimum detection concentration of DNA for F. oxysporum. The amplified products could be detected with the naked eye after completion of the reaction with SYBR green I. We were better able to control the effect of soft rot in D. officinale using fungicides following a positive test result. Additionally, the control effect of synergism combinations against soft rot was higher than 75%. Thus, LAMP assays could detect F. oxysporum in infected tissues of D. officinale and soils in field, allowing for early diagnosis of the disease.

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Section: Discussionmentioning

confidence: 99%

Detection and Control of Fusarium oxysporum from Soft Rot in Dendrobium officinale by Loop-Mediated Isothermal Amplification Assays

Xiao

2021

Biology

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“…During the last 100 years, the country has witnessed epidemics of various diseases like smut, pokkah boeng, red rot, wilt, and yellow leaf. The damage caused to sugarcane during each epidemic depends upon the nature of disease and spread of the susceptible varieties [24,25]. The incidence of diseases is increasing at an alarming rate, and the yield is declining every year.…”

Section: Comprehensive Control Of Diseases Pests and Weedsmentioning

confidence: 99%

Sugarcane Production in China

Govindaraju¹

2018

Sugarcane - Technology and Research

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Sugarcane production in China has a prolonged history since fourth century BC. At present, China is the world's third largest sugar producing country after Brazil and India. During the past decade, more than 90% of the sugar production was contributed by sugarcane. Guangxi is the dominant sugarcane and sugar producer, accounting for 65% of sugar production in China. China's sugarcane production faced serious problems in the past several years, especially the rapid increase in the labor cost because of the manual harvest. Now, China requires changing their sugarcane practice from manual to mechanical in order to catch up with the international trends in worldwide industry. Many other challenges and constraints are becoming severe, including abiotic and biotic stress, cost escalation, over fertilization, poor ratooning, and single cultivar. New technologies will be applied to sugarcane production, including omics-based breeding, best management practices, and so on.

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“…Sugarcane Sugarcane pokkah boengis named according to the symptoms of deformed or twisted tips (Wang, 2022), and the currently reported Sugarcane pokkah boengpathogens include Fusarium verticillioides Fusarium proliferatum Fusarium oxysporum Fusarium andiyazi Fusarium subglutinans Fusarium incarnatum Fusarium sacchari Fusarium fujikuroi Fusarium commune Fusarium miscanthi Fusarium nisikadoi (Mohammadi et Wang et al,2018). In China, Bao et al (2016) reported the sugarcane Sugarcane pokkah boengpathogen F. proliferatum for the rst time, Bao et al (2020) reported the sugarcane Sugarcane pokkah boengpathogens F. andiyazi and F. sacchari for the rst time, Wang et al (2018) reported the sugarcane Sugarcane pokkah boengpathogen F. commune for the rst time, and Wang (2022) reported the sugarcane pokkah boeng pathogen F. commune for the rst time. Wang (2022) reported the sugarcane Sugarcane pokkah boengpathogens F. miscanthi and F. nisikadoi.…”

Section: Introductionmentioning

confidence: 99%

Multi-gene identification and pathogenicity analysis of sugarcane pokkah boeng in Yunnan Province

Wang,

Yin,

et al. 2024

Preprint

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Sugarcane pokkah boeng disease is a fungal disease caused by Fusarium oxysporum. Eighty-seven samples of sugarcane pokkah boeng disease were isolated from Kaiyuan, Jinggu, Menglian, Yongde and Zhenkang sugarcane districts in Yunnan, and PCR amplification of the isolated Fusarium species was carried out by utilizing the sequences of the TEF, RPB2 and TUB2genes, and the results showed clear and bright bands consistent with the size of the target bands. The products were sequenced and analyzed by BLAST on NCBI website, and the sequences of strains with high homology were obtained to construct a phylogenetic tree with them. Combined with field symptom diagnosis, molecular characterization and morphological observation, five pathogenic bacteria causing sugarcane pokkah boeng were identified, namely Fusarium incarnatum、Fusarium andiyazi、Fusarium sacchari、Fusariumproliferatum and Fusarium verticillioides. F. incarnatum、 F. andiyazi and F. sacchari are reported for the first time in Yunnan sugarcane area. The mycelial morphology and conidial size of the five pathogenic bacteria did not differ much, and it was difficult to identify the causal agent of sugarcane tip rot by the difference in conidial size. The results of pathogenicity analysis by isolated inoculation showed that all five pathogens were able to infect and cause Sugarcane pokkah boengsymptoms in sugarcane, among which F. proliferatum was the most pathogenic, and the length and expansion of the spots were significantly higher than the others; on the contrary, F. verticillioides was the weakest pathogen. This study further clarified the types of F. verticillioides pathogens and their pathogenicity in Yunnan cane area, which provides scientific basis for the prevention and control of sugarcane pokkah boeng.

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First Report of Fusarium oxysporum Isolate gx3 Causing Sugarcane Pokkah Boeng in Guangxi of China

Cited by 12 publications

References 4 publications

Detection and Control of Fusarium oxysporum from Soft Rot in Dendrobium officinale by Loop-Mediated Isothermal Amplification Assays

Detection and Control of Fusarium oxysporum from Soft Rot in Dendrobium officinale by Loop-Mediated Isothermal Amplification Assays

Sugarcane Production in China

Multi-gene identification and pathogenicity analysis of sugarcane pokkah boeng in Yunnan Province

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