Detection and Control of Fusarium oxysporum from Soft Rot in Dendrobium officinale by Loop-Mediated Isothermal Amplification Assays

Xiao, Caiyun; Li, Rongyu

doi:10.3390/biology10111136

Cited by 12 publications

(11 citation statements)

References 60 publications

(58 reference statements)

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“…Based on this, the LAMP technology is a good strategy for the early diagnosis of bacterial diseases, which can prevent plant disease and pathogens during the preliminary stage and aids in health control and yield optimization without depending on the pest and pesticides usage (Le and Vu, 2017 ). Xiao and Li ( 2021 ) detected the soft rot pathogen of Dendrobium officinale by the LAMP method and then tested the control effect of several fungicides. After 14 days of spraying, the synergistic efficacy of the two fungicides (pyraclostrobin and picoxystrobin) reached 82.39%.…”

Section: Discussionmentioning

confidence: 99%

Detection and Control of Pantoea agglomerans Causing Plum Bacterial Shot-Hole Disease by Loop-Mediated Isothermal Amplification Technique

et al. 2022

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Plum bacterial shot-hole caused by Pantoea agglomerans (P. agglomerans) is one of the primary bacterial diseases in plum tree planting areas, resulting in abnormal growth of plum trees and severe economic losses. Early diagnosis of P. agglomerans is crucial to effectively control plant diseases. In this study, loop-mediated isothermal amplification (LAMP) analysis for genome-specific gene sequences was developed for the specific detection of P. agglomerans. We designed the LAMP primers based on the gyrB gene of P. agglomerans. The best reaction system was 0.2 μmol·L−1 for outer primer F3/B3 and 1.6 μmol·L−1 for inner primer FIP/BIP. The LAMP reaction was optimal at 65°C for 60 min based on the color change and gel electrophoresis. This technology distinguished P. agglomerans from other control bacteria. The detection limit of the LAMP technology was 5 fg·μl−1 genomic DNA of P. agglomerans, which is 1,000 times that of the traditional PCR detection method. The LAMP technology could effectively detect the DNA of P. agglomerans from the infected leaves without symptoms after indoor inoculation. Furthermore, the LAMP technology was applied successfully to detect field samples, and the field control effect of 0.3% tetramycin after LAMP detection reached 82.51%, which was 7.90% higher than that of conventional control. The proposed LAMP detection technology in this study offers the advantages of ease of operation, visibility of results, rapidity, accuracy, and high sensitivity, making it suitable for the early diagnosis of plum bacteria shot-hole disease.

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Section: Discussionmentioning

confidence: 99%

Detection and Control of Pantoea agglomerans Causing Plum Bacterial Shot-Hole Disease by Loop-Mediated Isothermal Amplification Technique

et al. 2022

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“…oxysporum , always causes stem rot of D . officinale and has a high incidence of 30% to 50% [ 8 , 40 ]. The medicinal part of D .…”

Section: Discussionmentioning

confidence: 99%

“…With the development of D. officinale industrialization, it is urgent to improve the resistance of tissue culture seedlings by genetic engineering. It is common for D. officinale to be exposed to various pathogens, including fungus, bacteria, and viruses during industrial cultivation [6][7][8][9]. For example, the common and destructive fungal pathogen, F. oxysporum, always causes stem rot of D. officinale and has a high incidence of 30% to 50% [8,40].…”

Section: Nbs-lrr Genes Play Important Roles In D Officinale Immune Sy...mentioning

confidence: 99%

“…Because of the great demand and the lack of wild resources, industrial cultivation of D. officinale has been actively promoted in Anhui, Zhejiang, Jiangsu and Guizhou provinces. However, the invasions of pathogens, such as orchid fleck virus, Dendrobium vein necrosis closterovirus, Fusarium oxysporum and Fusarium kyushuense, have led to production reduction, which resulted in great losses for enterprises [6][7][8][9]. Therefore, it is important to identify disease resistance (R) genes and explore the metabolic pathways of resistance to biotic stress based on the D. officinale genome.…”

Section: Introductionmentioning

confidence: 99%

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Evolution patterns of NBS genes in the genus Dendrobium and NBS-LRR gene expression in D. officinale by salicylic acid treatment

et al. 2022

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Background Dendrobium officinale Kimura et Migo, which contains rich polysaccharides, flavonoids and alkaloids, is a Traditional Chinese Medicine (TCM) with important economic benefits, while various pathogens have brought huge losses to its industrialization. NBS gene family is the largest class of plant disease resistance (R) genes, proteins of which are widely distributed in the upstream and downstream of the plant immune systems and are responsible for receiving infection signals and regulating gene expression respectively. It is of great significance for the subsequent disease resistance breeding of D. officinale to identify NBS genes by using the newly published high-quality chromosome-level D. officinale genome. Results In this study, a total of 655 NBS genes were uncovered from the genomes of D. officinale, D. nobile, D. chrysotoxum, V. planifolia, A. shenzhenica, P. equestris and A. thaliana. The phylogenetic results of CNL-type protein sequences showed that orchid NBS-LRR genes have significantly degenerated on branches a and b. The Dendrobium NBS gene homology analysis showed that the Dendrobium NBS genes have two obvious characteristics: type changing and NB-ARC domain degeneration. Because the NBS-LRR genes have both NB-ARC and LRR domains, 22 D. officinale NBS-LRR genes were used for subsequent analyses, such as gene structures, conserved motifs, cis-elements and functional annotation analyses. All these results suggested that D. officinale NBS-LRR genes take part in the ETI system, plant hormone signal transduction pathway and Ras signaling pathway. Finally, there were 1,677 DEGs identified from the salicylic acid (SA) treatment transcriptome data of D. officinale. Among them, six NBS-LRR genes (Dof013264, Dof020566, Dof019188, Dof019191, Dof020138 and Dof020707) were significantly up-regulated. However, only Dof020138 was closely related to other pathways from the results of WGCNA, such as pathogen identification pathways, MAPK signaling pathways, plant hormone signal transduction pathways, biosynthetic pathways and energy metabolism pathways. Conclusion Our results revealed that the NBS gene degenerations are common in the genus Dendrobium, which is the main reason for the diversity of NBS genes, and the NBS-LRR genes generally take part in D. officinale ETI system and signal transduction pathways. In addition, the D. officinale NBS-LRR gene Dof020138, which may have an important breeding value, is indirectly activated by SA in the ETI system.

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“…When compared with conventional PCR, LAMP offers obvious advantages of visualisation through turbidity or colour changes [ 27 , 34 , 35 ]. LAMP technology is currently widely used for the rapid detection of pathogens such as fungi [ 36 , 37 , 38 , 39 ], bacteria [ 40 , 41 , 42 ] and viruses [ 43 , 44 ].…”

Section: Introductionmentioning

confidence: 99%

First Report of Crown Gall of Kiwifruit (Actinidia deliciosa) Caused by Agrobacterium fabacearum in China and the Establishment of Loop-Mediated Isothermal Amplification Technique

Shi

Zhang

et al. 2021

IJMS

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Kiwifruit is moderately sweet and sour and quite popular among consumers; it has been widely planted in some areas of the world. In 2019, the crown gall disease of kiwifruit was discovered in the main kiwifruit-producing area of Guizhou Province, China. This disease can weaken and eventually cause the death of the tree. The phylogeny, morphological and biological characteristics of the bacteria were described, and were related to diseases. The pathogenicity of this species follows the Koch hypothesis, confirming that A. fabacearum is the pathogen of crown gall disease of kiwifruit in China. In this study, Loop-mediated isothermal amplification (LAMP) analysis for genome-specific gene sequences was developed for the specific detection of A. fabacearum. The detection limit of the LAMP method is 5 × 10−7 ng/μL, which has high sensitivity. At the same time, the amplified product is stained with SYBR Green I after the reaction is completed, so that the amplification can be detected with the naked eye. LAMP analysis detected the presence of A. fabacearum in the roots and soil samples of the infected kiwifruit plant. The proposed LAMP detection technology in this study offers the advantages of ease of operation, visibility of results, rapidity, accuracy and high sensitivity, making it suitable for the early diagnosis of crown gall disease of kiwifruit.

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Detection and Control of Fusarium oxysporum from Soft Rot in Dendrobium officinale by Loop-Mediated Isothermal Amplification Assays

Cited by 12 publications

References 60 publications

Detection and Control of Pantoea agglomerans Causing Plum Bacterial Shot-Hole Disease by Loop-Mediated Isothermal Amplification Technique

Detection and Control of Pantoea agglomerans Causing Plum Bacterial Shot-Hole Disease by Loop-Mediated Isothermal Amplification Technique

Evolution patterns of NBS genes in the genus Dendrobium and NBS-LRR gene expression in D. officinale by salicylic acid treatment

First Report of Crown Gall of Kiwifruit (Actinidia deliciosa) Caused by Agrobacterium fabacearum in China and the Establishment of Loop-Mediated Isothermal Amplification Technique

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