First Report of <i>Didymella lethalis</i> Associated with Roots of Pea, Subterranean Clover, and Winter Vetch in Germany, Switzerland, and Italy

Šišić, Adnan; Baćanović-Šišić, Jelena; Schmidt, Harald; Finckh, Maria R.

doi:10.1094/pdis-05-18-0837-pdn

Cited by 3 publications

(5 citation statements)

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“…Although not evaluated by Davidson et al [22], their primers and probe set would likely crossreact with D. lethalis as the species has an identical ITS sequence with the former two species. In addition to a high level of morphological and genetic similarity, D. pinodella, D. pinodes, and D. lethalis occupy similar ecological niches and can occur together in plant and environmental samples [17,38].…”

Section: Discussionmentioning

confidence: 99%

“…All isolates in the internal University of Kassel culture collection were characterized morphologically, and their taxonomic identity was confirmed by sequencing the ITS, beta tubulin, actin and/or RPB2 genes for Didymella isolates and related species, and the TEF-1 alpha gene region for Fusarium spp. [5,15,17,23,24].…”

Section: Fungal Isolatesmentioning

confidence: 99%

“…Culture-based fungal re-isolations and morphological identifications were performed as described previously [5,17] using four pea and five wheat roots randomly selected from each treatment. The same number of roots representing each treatment was used for DNA extractions and qPCR assay validation in infected plant tissue.…”

Section: Production Of Infected Plant Materialsmentioning

confidence: 99%

“…are relatively slow growing fungi that are commonly overgrown by fast growers, requiring frequent sub-culturing. Furthermore, in addition to D. pinodella, the less frequent, closely related D. pinodes [2] and D. lethalis [17] were also reported as a part of the pea root rot complex, and are likely to co-occur on other grain legumes as well. The three Didymella species share many important cultural and morphological characteristics and discrimination of these pathogens based on the morphology alone can be difficult [18,19].…”

Section: Introductionmentioning

confidence: 99%

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A Novel Real Time PCR Method for the Detection and Quantification of Didymella pinodella in Symptomatic and Asymptomatic Plant Hosts

Šišić

Oberhänsli

Baćanović-Šišić

et al. 2021

JoF

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Didymella pinodella is the major pathogen of the pea root rot complex in Europe. This wide host range pathogen often asymptomatically colonizes its hosts, making the control strategies challenging. We developed a real-time PCR assay for the detection and quantification of D. pinodella based on the TEF-1 alpha gene sequence alignments. The assay was tested for specificity on a 54-isolate panel representing 35 fungal species and further validated in symptomatic and asymptomatic pea and wheat roots from greenhouse tests. The assay was highly consistent across separate qPCR reactions and had a quantification/detection limit of 3.1 pg of target DNA per reaction in plant tissue. Cross-reactions were observed with DNA extracts of five Didymella species. The risk of cross contamination, however, is low as the non-targets have not been associated with pea previously and they were amplified with at least 1000-fold lower sensitivity. Greenhouse inoculation tests revealed a high correlation between the pathogen DNA quantities in pea roots and pea root rot severity and biomass reduction. The assay also detected D. pinodella in asymptomatic wheat roots, which, despite the absence of visible root rot symptoms, caused wheat biomass reduction. This study provides new insights into the complex life style of D. pinodella and can assist in better understanding the pathogen survival and spread in the environment.

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Section: Discussionmentioning

confidence: 99%

Section: Fungal Isolatesmentioning

confidence: 99%

Section: Production Of Infected Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Novel Real Time PCR Method for the Detection and Quantification of Didymella pinodella in Symptomatic and Asymptomatic Plant Hosts

Šišić

Oberhänsli

Baćanović-Šišić

et al. 2021

JoF

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“…Rhizoctonia solani , Thielaviopsis basicola and Didymella spp. (responsible for pea foot rot and ascochyta blight) have also been frequently reported as causal agents of root rot (Chatterton et al, 2019; Oyarzun et al, 1993; Persson et al, 1997; Šišić, Baćanović‐Šišić, Schmidt, & Finckh, 2018). Other fungi, such as Sclerotinia sclerotiorium or Cylindrocarpon spp., have only been found sporadically in infected tissues and have never been described to have a major impact on crop yields (Oyarzun et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Identification, pathogenicity and community dynamics of fungi and oomycetes associated with pea root rot in northern France

et al. 2022

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The pea root rot complex is a major concern for green pea production worldwide. This study aimed at characterizing its composition and dynamics throughout a cropping season in northern France. To this end, fungi and oomycetes were isolated from green pea plant roots with symptoms sampled at the flowering stage in 22 fields in 2017, and at the pea emergence, elongation and flowering stages in two fields in 2018. Out of 646 isolates collected, 317 were identified using molecular markers. Fusarium oxysporum, F. solani and F. redolens were highly predominant. Pathogenicity tests separated the isolates into four aggressiveness groups. F. solani isolates were the most aggressive. Phylogenetic analysis of their TEF1 sequences showed that they mainly belonged to the F. pisi lineage, and that F. oxysporum isolates were genetically close to isolates from the UK that did not belong to the forma specialis pisi. In addition, several Clonostachys rhizophaga isolates are reported for the first time to cause pea root rot. The oomycetes were rarely found and were represented by a few Pythium spp. isolates. Lastly, this study shows that the fungal and oomycete communities associated with pea root rot change during the cropping season. The level of dissimilarity of the root‐rot‐associated communities decreased throughout the cropping season towards a more similar composition at the flowering stage, dominated by F. solani, F. oxysporum and F. redolens. The proportion of nonpathogenic to weakly pathogenic isolates decreased progressively during the growing season in favour of moderately to highly pathogenic isolates.

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Root Pathogens Occurring on Pea (Pisum sativum) and Faba Bean (Vicia faba) in Germany

Šišić

Baćanović-Šišić

Schmidt³

et al. 2020

30th Scientific-Experts Conference of Agriculture and Food Industry

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First Report of Didymella lethalis Associated with Roots of Pea, Subterranean Clover, and Winter Vetch in Germany, Switzerland, and Italy

Cited by 3 publications

References 3 publications

A Novel Real Time PCR Method for the Detection and Quantification of Didymella pinodella in Symptomatic and Asymptomatic Plant Hosts

A Novel Real Time PCR Method for the Detection and Quantification of Didymella pinodella in Symptomatic and Asymptomatic Plant Hosts

Identification, pathogenicity and community dynamics of fungi and oomycetes associated with pea root rot in northern France

Root Pathogens Occurring on Pea (Pisum sativum) and Faba Bean (Vicia faba) in Germany

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