Root Pathogens Occurring on Pea (Pisum sativum) and Faba Bean (Vicia faba) in Germany

Šišić, Adnan; Baćanović-Šišić, Jelena; Schmidt, Harald; Finckh, Maria R.

doi:10.1007/978-3-030-40049-1_8

Cited by 6 publications

(11 citation statements)

References 15 publications

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Section: Discussionmentioning

confidence: 80%

“…Our recent study showed particular dominance of this species in roots of winter pea varieties in Germany, which were found surprisingly less frequently colonized by Fusarium spp. [14]. We also found D. pinodella in asymptomatic vetch, clover and wheat roots [5,15].…”

Section: Introductionmentioning

confidence: 66%

“…Similar challenges exist with isolation and identification of plant associated Didymella spp. For example, in Europe, D. pinodella is one of the most dominant pathogens associated with root rots of pea and faba bean [2,8,14]. Our recent study showed particular dominance of this species in roots of winter pea varieties in Germany, which were found surprisingly less frequently colonized by Fusarium spp.…”

Section: Introductionmentioning

confidence: 85%

“…This study was prompted by reports which indicated that D. pinodella is an important part of the pea root rot complex in Germany, Denmark, and Sweden [2,8,14,36]. Recently, we also found high abundance of this species in symptomatic and asymptomatic pea roots from France and Hungary (Šišić et al, unpublished data) as well as in asymptomatic faba bean, vetch, clover, and wheat roots grown in different regions of Europe [5,14,15]. Given the limitations of culture based methods often coupled with the labor-intensive PCR amplifications, Sanger sequencing and phylogenetic studies, we designed and validated a novel probe-based qPCR assay for detecting and quantification of this pathogen.…”

Section: Discussionmentioning

confidence: 99%

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A Novel Real Time PCR Method for the Detection and Quantification of Didymella pinodella in Symptomatic and Asymptomatic Plant Hosts

Šišić

Oberhänsli

Baćanović-Šišić

et al. 2021

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Didymella pinodella is the major pathogen of the pea root rot complex in Europe. This wide host range pathogen often asymptomatically colonizes its hosts, making the control strategies challenging. We developed a real-time PCR assay for the detection and quantification of D. pinodella based on the TEF-1 alpha gene sequence alignments. The assay was tested for specificity on a 54-isolate panel representing 35 fungal species and further validated in symptomatic and asymptomatic pea and wheat roots from greenhouse tests. The assay was highly consistent across separate qPCR reactions and had a quantification/detection limit of 3.1 pg of target DNA per reaction in plant tissue. Cross-reactions were observed with DNA extracts of five Didymella species. The risk of cross contamination, however, is low as the non-targets have not been associated with pea previously and they were amplified with at least 1000-fold lower sensitivity. Greenhouse inoculation tests revealed a high correlation between the pathogen DNA quantities in pea roots and pea root rot severity and biomass reduction. The assay also detected D. pinodella in asymptomatic wheat roots, which, despite the absence of visible root rot symptoms, caused wheat biomass reduction. This study provides new insights into the complex life style of D. pinodella and can assist in better understanding the pathogen survival and spread in the environment.

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Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 66%

Section: Introductionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

A Novel Real Time PCR Method for the Detection and Quantification of Didymella pinodella in Symptomatic and Asymptomatic Plant Hosts

Šišić

Oberhänsli

Baćanović-Šišić

et al. 2021

JoF

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“…In most places, A. euteiches, three or four Fusarium species, and occasionally Didymella spp. are among the greatest concerns in pea production (Chatterton et al, 2019;Persson et al, 1997;Šišić et al, 2020). In the UK, the parasitic complex is believed to be restricted to F. oxysporum, F. solani and F. redolens (Jenkins, 2018).…”

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confidence: 99%

Identification, pathogenicity and community dynamics of fungi and oomycetes associated with pea root rot in northern France

et al. 2022

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The pea root rot complex is a major concern for green pea production worldwide. This study aimed at characterizing its composition and dynamics throughout a cropping season in northern France. To this end, fungi and oomycetes were isolated from green pea plant roots with symptoms sampled at the flowering stage in 22 fields in 2017, and at the pea emergence, elongation and flowering stages in two fields in 2018. Out of 646 isolates collected, 317 were identified using molecular markers. Fusarium oxysporum, F. solani and F. redolens were highly predominant. Pathogenicity tests separated the isolates into four aggressiveness groups. F. solani isolates were the most aggressive. Phylogenetic analysis of their TEF1 sequences showed that they mainly belonged to the F. pisi lineage, and that F. oxysporum isolates were genetically close to isolates from the UK that did not belong to the forma specialis pisi. In addition, several Clonostachys rhizophaga isolates are reported for the first time to cause pea root rot. The oomycetes were rarely found and were represented by a few Pythium spp. isolates. Lastly, this study shows that the fungal and oomycete communities associated with pea root rot change during the cropping season. The level of dissimilarity of the root‐rot‐associated communities decreased throughout the cropping season towards a more similar composition at the flowering stage, dominated by F. solani, F. oxysporum and F. redolens. The proportion of nonpathogenic to weakly pathogenic isolates decreased progressively during the growing season in favour of moderately to highly pathogenic isolates.

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Legume root diseases

2024

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Root Pathogens Occurring on Pea (Pisum sativum) and Faba Bean (Vicia faba) in Germany

Cited by 6 publications

References 15 publications

A Novel Real Time PCR Method for the Detection and Quantification of Didymella pinodella in Symptomatic and Asymptomatic Plant Hosts

A Novel Real Time PCR Method for the Detection and Quantification of Didymella pinodella in Symptomatic and Asymptomatic Plant Hosts

Identification, pathogenicity and community dynamics of fungi and oomycetes associated with pea root rot in northern France

Legume root diseases

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