First Report of <i>Diaporthe rudis</i> in Chile Causing Stem-End Rot on ‘Hass’ Avocado Fruit Imported From California

Torres, Carolina A.; Camps, Rocío; Aguirre, R.; Besoaín, Ximena

doi:10.1094/pdis-12-15-1495-pdn

Cited by 11 publications

(8 citation statements)

References 2 publications

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“…Diaporthe spp. such as D. foeniculina, D. sterili and D. radis have been reported from SER of avocado fruits by several researchers previously (Guarnaccia et al, 2016;Torres et al, 2016). Therefore, this is the first record of identification of D. nelumbonis from avocado fruits in Sri Lanka.…”

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confidence: 55%

Morphological and molecular identification of fungal species associated with postharvest stem-end rot disease of avocado in Sri Lanka

et al. 2020

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Avocado (Persia americana) is one of the most popular fruits grown in Sri Lanka. Postharvest infections occur wherever the crops are cultivated. Among them, stem-end rot (SER) is the major disease reported. Several fungal species including Lasiodiplodia and Dothiorella spp. have been reported to be associated with this disease in regions where avocado is grown. The aims of this research were to identify the fungal pathogens associated with the stem-end rot of avocado in Sri Lanka based on morphological characteristics in combination with molecular and phylogenetic analysis. Diseased avocado fruits were collected from local markets and causal agents were isolated. Colony morphology and characteristics of conidia were observed using phase-contrast microscopy. Sequence analysis was performed using internal transcribed spacers (ITS) of the ribosomal DNA followed by phylogenetic analysis. Four endophytic fungal isolates were identified and designated as SER 01, SER 02, SER 03 and SER 04 which were distinguished respectively as Lasiodiplodia theobromae, Lasiodiplodia hormozganensis, Diaporthe nelumbonis and Fusarium oxysporum. To our knowledge, this is the first evidence on the occurrence of L. hormozganensis, D. nelumbonis and F. oxysporum associated with SER of avocado in Sri Lanka. Identified pathogens were proven to be collectively pathogenic to avocado following demonstration of Koch's postulates. The average value of collective disease severity after seven days of inoculation of avocado cv. Pollock was 40% at 28 C and it always fluctuated between 30% and 45%.

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confidence: 55%

Morphological and molecular identification of fungal species associated with postharvest stem-end rot disease of avocado in Sri Lanka

et al. 2020

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“…Furthermore, species of Diaporthe are commonly introduced into new areas as endophytes or latent pathogens along with plant produce. For instance, Torres et al . (2016) reported D. rudis causing stem-end rot in avocados in Chile, which was imported via avocado fruit from California (USA).…”

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confidence: 99%

Diaporthe is paraphyletic

et al. 2017

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Previous studies have shown that our understanding of species diversity within Diaporthe (syn. Phomopsis) is limited. In this study, 49 strains obtained from different countries were subjected to DNA sequence analysis. Based on these results, eight new species names are introduced for lineages represented by multiple strains and distinct morphology. Twelve Phomopsis species previously described from China were subjected to DNA sequence analysis, and confirmed to belong to Diaporthe. The genus Diaporthe is shown to be paraphyletic based on multi-locus (LSU, ITS and TEF1) phylogenetic analysis. Several morphologically distinct genera, namely Mazzantia, Ophiodiaporthe, Pustulomyces, Phaeocytostroma, and Stenocarpella, are embedded within Diaporthe s. lat., indicating divergent morphological evolution. However, splitting Diaporthe into many smaller genera to achieve monophyly is still premature, and further collections and phylogenetic datasets need to be obtained to address this situation.

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“…They are widely distributed and have a broad range of hosts from economically significant agricultural crops to ornamental plants including Camellia , Castanea , Citrus , Glycine , Helianthus , Juglans , Persea , Pyrus , Vaccinium and Vitis ( van Rensburg et al 2006 ; Santos and Phillips 2009 ; Crous et al 2011a , b , 2016 ; Santos et al 2011 ; Thompson et al 2011 ; Grasso et al 2012 ; Huang et al 2013 ; Lombard et al 2014 ; Gao et al 2015 , 2016 , 2017 ; Udayanga et al 2012 , 2015 ; Guarnaccia et al 2016 ; Dissanayake et al 2017 ; Guarnaccia and Crous 2017 ; Fan et al 2018 ; Senanayake et al 2018 ; Guo et al 2020 ). Many Diaporthe species have been reported as destructive plant pathogens, innocuous endophytes and saprobes ( Murali et al 2006 ; Udayanga et al 2012 ; Gomes et al 2013 ; Ménard et al 2014 ; Guarnaccia et al 2016 ; Torres et al 2016 ; Senanayake et al 2018 ). However, the biology and lifestyle of some of them remain unclear ( Vilka and Volkova 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species

Sun¹,

Huang²,

Xia³

et al. 2021

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Diaporthe species have often been reported as plant pathogens, endophytes and saprophytes, commonly isolated from a wide range of infected plant hosts. In the present study, twenty strains obtained from leaf spots of twelve host plants in Yunnan Province of China were isolated. Based on a combination of morphology, culture characteristics and multilocus sequence analysis of the rDNA internal transcribed spacer region (ITS), translation elongation factor 1-α (TEF), β-tubulin (TUB), calmodulin (CAL), and histone (HIS) genes, these strains were identified as eight new species: Diaporthe camelliae-sinensis, D. grandiflori, D. heliconiae, D. heterostemmatis, D. litchii, D. lutescens, D. melastomatis, D. pungensis and two previously described species, D. subclavata and D. tectonendophytica. This study showed high species diversity of Diaporthe in tropical rain forests and its hosts in south-western China.

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First Report of Diaporthe rudis in Chile Causing Stem-End Rot on ‘Hass’ Avocado Fruit Imported From California

Cited by 11 publications

References 2 publications

Morphological and molecular identification of fungal species associated with postharvest stem-end rot disease of avocado in Sri Lanka

Morphological and molecular identification of fungal species associated with postharvest stem-end rot disease of avocado in Sri Lanka

Diaporthe is paraphyletic

Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species

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