Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species

Abstract

Diaporthe species have often been reported as plant pathogens, endophytes and saprophytes, commonly isolated from a wide range of infected plant hosts. In the present study, twenty strains obtained from leaf spots of twelve host plants in Yunnan Province of China were isolated. Based on a combination of morphology, culture characteristics and multilocus sequence analysis of the rDNA internal transcribed spacer region (ITS), translation elongation factor 1-α (TEF), β-tubulin (TUB), calmodulin (CAL), and histone (HIS) genes, these strains were identified as eight new species: Diaporthe camelliae-sinensis, D. grandiflori, D. heliconiae, D. heterostemmatis, D. litchii, D. lutescens, D. melastomatis, D. pungensis and two previously described species, D. subclavata and D. tectonendophytica. This study showed high species diversity of Diaporthe in tropical rain forests and its hosts in south-western China.

Two new species of Diaporthe (Diaporthaceae, Diaporthales) associated with tree cankers in the Netherlands

“…Phylogenetic analyses were conducted based on a combined DNA sequence matrix of five loci ( ITS , cal , his3 , tef1 and tub2 ) reported as useful markers to distinguish species of Diaporthe ( Udayanga et al 2014 , 2015 ; Guarnaccia et al 2017, 2018a, 2018b ; Tibpromma et al 2018 ; Yang et al 2020 ; Dissanayake et al 2020 ; Huang et al 2021 ; Sun et al 2021 , Wang et al 2021 ). The two novel species in this study can be distinguished from the other known species by all genes studied, but most effectively by cal , his3 , tef1 and tub2 .…”

“…The quality of the amplified nucleotide sequences was checked and the sequences assembled using SeqMan v.7.1.0. Reference sequences were retrieved from the National Center for Biotechnology Information ( NCBI ), based on recent publications on the genus Diaporthe (Dissanayake et al 2021; Gao et al 2021 ; Huang et al 2021 ; Sun et al 2021 , Wang et al 2021 ; Yang et al 2021 ). Sequences were aligned using MAFFT v. 6 ( Katoh and Toh 2010 ) and corrected manually using MEGA 7.0.21.…”

Biocontrol Potential and Catabolic Profile of Endophytic Diaporthe eres Strain 1420S from Prunus domestica L. in Poland—A Preliminary Study

“…Diaporthe Nitschke, and its asexual Phomopsis-like morph have been often described as plant pathogens, saprobes, and endophytes in a wide range of plant hosts [10,[12][13][14][15][16][17]. For many years, their identification has been based on morphological features and host-plant associations [12].…”

“…For many years, their identification has been based on morphological features and host-plant associations [12]. Recently, multi-locus DNA data analysis combined with morphological characterization and host associations has been employed for species delimitation in the genus [13,15,[17][18][19][20][21][22][23][24][25][26][27][28][29]. Although several endophytic Diaporthe species have been described as endophytes on woody plants worldwide [3,20,[30][31][32][33][34][35], there is no available information concerning endophytic Diaporthe on Prunus domestica in Poland or worldwide.…”

Characterization of Diaporthe species associated with peach constriction canker, with two novel species from China

“…Therefore, molecular data have been adopted to resolve the circumscription of species of Diaporthe, initially relying on the internal transcribed spacer (ITS) of the ribosomal DNA region (Santos et al 2009; Thompson et al 2011), and recently on multiple loci including ITS, translation elongation factor 1-α (TEF), ß-tubulin (TUB), histone (HIS), and calmodulin (CAL) gene regions (Santos et al 2017). At present, the five-locus dataset (ITS-TEF-CAL-HIS-TUB) has been optimally adopted for the species delimitation by recent authors (Gao et al 2017;Yang et al 2018Yang et al , 2020Crous et al 2020;Guo et al 2020;Hyde et al 2020;Sun et al 2021).…”