First Report of Fusarium Wilt of Blackberry Caused by <i>Fusarium oxysporum</i> f. sp. <i>mori</i> in North Carolina

Pastrana, Ana M.; Cline, W. O.; Wong, T. W.; Watson, Dean C.; Mercier, Jean; Ivors, Kelly; Broome, Janet C.; Quesada‐Ocampo, Lina M.; Gordon, Thomas R.

doi:10.1094/pdis-09-19-1980-pdn

Cited by 5 publications

(3 citation statements)

References 6 publications

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“…The seriously damaged crops reported at home and abroad are eggplant, sugarcane, blackberry, cotton, etc. [ 43 , 44 , 45 , 46 ]. At present, chemical control is also the most common method to control plant diseases.…”

Section: Discussionmentioning

confidence: 99%

“…For the optimization of reagents, a range of reaction temperatures (55, 58, 60, 62, 64, 65, 66, 68, and 70 • C), a range of reaction times (15,30,45,60,75, and 90 min), a range of Mg 2+ concentrations (2, 3, 4, 5, 6, 7, 8, 9, and 10 mM), a range of inner primer concentrations (0.4, 0.8, 1.2, 1.6, and 2 µM), and a range of outer primer concentrations (0.2, 0.4, 0.6, 0.8, and 1 µM) were evaluated under otherwise identical conditions. The LAMP reactions were performed in 200 µL microfuge tubes incubated in a water bath for 60 min at 65 • C. The reactions were halted by immersion at 80 • C for 5 min, at which point reaction products were detected by fluorescent LAMP and electrophoresis, following which LAMP reactions were completed.…”

Section: Optimization Of Lamp Reaction Conditionsmentioning

confidence: 99%

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Detection and Control of Fusarium oxysporum from Soft Rot in Dendrobium officinale by Loop-Mediated Isothermal Amplification Assays

Xiao

2021

Biology

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Soft rot causing Fusarium oxysporum is one of the most destructive diseases of Dendrobium officinale Kimura et Migo in China that reduces D. officinale yield and quality. A key challenge for an integrated management strategy for this disease is the rapid and accurate detection of F. oxysporum on D. officinale. Therefore, a new loop-mediated isothermal amplification (LAMP) assay was developed for this purpose. In this study, the primers were selected and designed using the translation elongation factor-1α (TEF-1α) gene region as the target DNA sequence in order to screen the best system of reaction of LAMP to detect F. oxysporum through optimizing different conditions of the LAMP reaction, including time, temperature, concentrations of MgSO4, and concentrations of inner and outer primers. The optimized system was able to efficiently amplify the target gene at 62 °C for 60 min with 1.2 μM internal primers, 0.4 μM external primers, 7 mM Mg2+, and 5 fg/µL minimum detection concentration of DNA for F. oxysporum. The amplified products could be detected with the naked eye after completion of the reaction with SYBR green I. We were better able to control the effect of soft rot in D. officinale using fungicides following a positive test result. Additionally, the control effect of synergism combinations against soft rot was higher than 75%. Thus, LAMP assays could detect F. oxysporum in infected tissues of D. officinale and soils in field, allowing for early diagnosis of the disease.

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Section: Discussionmentioning

confidence: 99%

Section: Optimization Of Lamp Reaction Conditionsmentioning

confidence: 99%

Detection and Control of Fusarium oxysporum from Soft Rot in Dendrobium officinale by Loop-Mediated Isothermal Amplification Assays

Xiao

2021

Biology

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“…Blackberry cultivation, particularly in the subtropical regions, faces major challenges from disease pressure (Agehara et al, 2020;Marin et al, 2022). These diseases include leaf rust caused by Kuehneola uredinis (Marin et al, 2022), leaf spot caused by Pseudocercospora pancratii (Marin et al, 2023), Fusarium wilt caused by Fusarium oxysporum (Pastrana et al, 2020), downy mildew caused by Peronospora sparsa (Ali et al, 2019), powdery mildew caused by Podosphaera aphanis (Solano-Báez et al, 2022), and orange cane blotch caused by the algae Cephaleuros virescens (Browne et al, 2020). Developing disease-resistant varieties is crucial for helping growers address diseases in a socioeconomically and environmentally friendly manner.…”

Section: Chloroplast Genomementioning

confidence: 99%

A chromosome-scale and haplotype-resolved genome assembly of tetraploid blackberry (RubusL. subgenusRubusWatson)

Paudel

Peng

Parajuli

et al. 2023

Preprint

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Blackberries (Rubus subgenus Rubus) are a major berry crop consumed globally for being a rich source of anthocyanins and antioxidants, and their unique flavor. However, breeding for fruit improvement in blackberry has been significantly hindered by the scarcity of genomic resources and the genetic complexity of traits. The blackberry genome has been particularly challenging to assemble, largely due to its polyploid nature. We present the first chromosome-scale and haplotype-phased genome assembly for the cultivated primocane-fruiting, thornless tetraploid blackberry selection BL1 (Rubus L. subgenus Rubus Watson). The tetraploid genome assembly was generated using the Oxford Nanopore Technology (ONT) and Hi-C scaffolding, comprising 919 Mb placed on 27 pseudochromosomes with an N50 of 35.73 Mb. The assembly covers >92% of the genome length and contains over 98% of complete BUSCOs. Repetitive sequences constitute 57% of the assembly, with the long terminal repeats (LTR) being the most abundant class. A total of 87,968 protein-coding genes were predicted, of which, 82% were functionally annotated. Gene expression analyses identified candidate genes and transcription factors related to thornlessness in blackberries, including MYB16, lysine histidine transporters, PDR1, Caffeoyl-CoA, glycosylphosphatidylinositol-anchored lipid protein transfer 1, DRN, beta-ketoacyl reductase, and homocysteine S-methyltransferase 3. The utility of this genome has been demonstrated in this study by identifying candidate genes related to thornlessness in blackberry. Resequencing of tetraploid blackberry cultivars/selections with different horticultural characteristics revealed genes that could impact fruiting habit and disease resistance/susceptibility. This tetraploid reference genome will serve as a valuable resource to accelerate genetic analysis and breeding of this important berry crop, enabling the development of improved varieties with enhanced traits.

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Fusarium oxysporum (basal rot)

2022

CABI Compendium

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First Report of Fusarium Wilt of Blackberry Caused by Fusarium oxysporum f. sp. mori in North Carolina

Cited by 5 publications

References 6 publications

Detection and Control of Fusarium oxysporum from Soft Rot in Dendrobium officinale by Loop-Mediated Isothermal Amplification Assays

Detection and Control of Fusarium oxysporum from Soft Rot in Dendrobium officinale by Loop-Mediated Isothermal Amplification Assays

A chromosome-scale and haplotype-resolved genome assembly of tetraploid blackberry (RubusL. subgenusRubusWatson)

Fusarium oxysporum (basal rot)

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