First Report of Fish Pasteurellosis from Malta

Bakopoulos, Vasileios; Peric, Zarco; Rodger, H. D.; Adams, Alexandra; Richards, R. H.

doi:10.1577/1548-8667(1997)009<0026:frofpf>2.3.co;2

Cited by 34 publications

(22 citation statements)

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“…Susceptibility to photobacteriosis has been reported in newly hatched sea bream larvae causing mortalities of 50e100% [1]. In the present study, brood fish were immunised twice before spawning.…”

Section: Discussionmentioning

confidence: 86%

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Maternal transfer of humoral specific and non-specific immune parameters to sea bream (Sparus aurata) larvae

Hanif

Bakopoulos

Dimitriadis

2004

Fish & Shellfish Immunology

151

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Section: Discussionmentioning

confidence: 86%

“…piscicida (Phdp) (formerly known as Pasteurella piscicida) causes serious economic losses in various wild and farmed fish species [1]. Fish larvae do not have the ability to develop specific immunity during the early stages of development [2].…”

Section: Introductionmentioning

confidence: 99%

Maternal transfer of humoral specific and non-specific immune parameters to sea bream (Sparus aurata) larvae

Hanif

Bakopoulos

Dimitriadis

2004

Fish & Shellfish Immunology

151

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“…piscicida has been detected by different methods , Bakopoulos et al 1995, 1997b, Thyssen et al 2000. With some of these methods the Japanese isolates were found to differ from the Mediterranean/European isolates (Bakopoulos et al 1997a, Magariños et al 1997, Thyssen et al 2000.…”

Section: Discussionmentioning

confidence: 99%

“…Sea bream Sparus aurata and sea bass Dicentrarchus labrax farms in particular have consequently suffered severe economic losses in several countries around the Mediterranean basin. The disease has been reported in Spain (Toranzo et al 1991), France (Baudin-Laurencin et al 1991), Italy (Ceschia et al 1991), Greece (Ghittino et al 1993), Turkey (Candan et al 1996), Malta (Bakopoulos et al 1997b) and Israel (A. Colorni unpubl. data).…”

Section: Introductionmentioning

confidence: 99%

Parasites of wild sea bass Dicentrarchus labrax from Norway

Kvitt

Ucko²,

Colorni³

et al. 2002

Dis. Aquat. Org.

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A PCR protocol for the rapid diagnosis of fish 'pasteurellosis' based on 16S rRNA gene sequences was developed. The procedure combines low annealing temperature that detects low titers of Photobacterium damselae but also related species, and high annealing temperature for the specific identification of P. damselae directly from infected fish. The PCR protocol was validated on 19 piscine isolates of P. damselae ssp. piscicida from different geographic regions (Japan, Italy, Spain, Greece and Israel), on spontaneously infected sea bream Sparus aurata and sea bass Dicentrarchus labrax, and on closely related American Type Culture Collection (ATCC) reference strains. PCR using high annealing temperature (64°C) discriminated between P. damselae and closely related reference strains, including P. histaminum. Sixteen isolates of P. damselae ssp. piscicida, 2 P. damselae ssp. piscicida reference strains and 1 P. damselae ssp. damselae reference strain were subjected to Amplified Fragment Length Polymorphism (AFLP) analysis, and a similarity matrix was produced. Accordingly, the Japanese isolates of P. damselae ssp. piscicida were distinguished from the Mediterranean/European isolates at a cut-off value of 83% similarity. A further subclustering at a cut-off value of 97% allowed discrimination between the Israeli P. damselae ssp. piscicida isolates and the other Mediterranean/European isolates. The combination of PCR direct amplification and AFLP provides a 2-step procedure, where P. damselae is rapidly identified at genus level on the basis of its 16S rRNA gene sequence and then grouped into distinct clusters on the basis of AFLP polymorphisms. The first step of direct amplification is highly sensitive and has immediate practical consequences, offering fish farmers a rapid diagnosis, while the AFLP is more specific and detects intraspecific variation which, in our study, also reflected geographic correspondence. Because of its superior discriminative properties, AFLP can be an important tool for epidemiological and taxonomic studies of this highly homogeneous genus. DISEASES OF AQUATIC ORGANISMS

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“…The IgM levels of Larval extracts of fish were assessed with ELISA by the method of Bakopoulos, Peric [22] with some modification. 50 μL of serial dilutions of the sera (1:50) were added to the appropriate micro titer plate wells with a HRP-conjugated IgM and antibody preparation specific for IgM and incubated.…”

Section: Enzyme Linked Immunosorbent Assay (Elisa) For Determination mentioning

confidence: 99%

Influence of Maternal and Larval Immunisation against <i>Lactococcus garviae</i> Infection in Rainbow Trout <i>Oncorhynchus mykiss</i> (Walaum) Lysozyme Activity and IgM Level

Akbary¹,

Mirvaghefi²,

Akhlaghi³

et al. 2015

OJAS

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How to cite this paper: Akbary, P., Mirvaghefi, A.R., Akhlaghi, M. and Fereidouni, M.S. (2015) AbstractThis study evaluated efficacy of maternal and larval immunisation against Lactococcus garviae infection and on the lysozyme and immunoglobulin (IgM) levels in rainbow trout Oncorhynchus mykiss (Walaum). Forty-eight-day-old larvae (mean weight 96 mg) originating from injected weekly with letrozole and immunised, only immunised and non-immunised parents were experimentally infected with the L. garvieae, and the mortality rate was recorded daily. Larvae were vaccinated by immersion at 58 days post hatch with live L. garvieae (10 9 cells/mL) for 15 min. Every third day post larvae vaccination, two larvae from each group were collected for analysis lysozyme (by a method based on the ability of lysozyme to lyse the bacterium Micrococcus lysodeikticus) and IgM (by enzyme-linked immunosorbent assay (ELISA)) parameters. Vaccinated and control larvae were tested for protection against L. garvieae 30 days post larvae immunization when the larvae were 88 days old. Larvae were challenged by bath exposure with live L. garvieae (10 9 cells/mL) for 2 min and monitored for mortality for at least 10 days following challenge. The challenge experiment with L. garvieae showed a significant reduction in larvae from immunised (54.44% ± 0.64%) and injected weekly with letrozole and immunised fish (52.96% ± 0.97%) compared to larvae from control fish (62.96% ± 2.22%). Vaccinated larvae originated from injected weekly with letrozole and immunised parents showed significantly higher lysozyme activity compared to other fish groups. Vaccinated larvae showed significantly less mortality compared to controls. The rela-* Corresponding author.

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First Report of Fish Pasteurellosis from Malta

Cited by 34 publications

References 0 publications

Maternal transfer of humoral specific and non-specific immune parameters to sea bream (Sparus aurata) larvae

Maternal transfer of humoral specific and non-specific immune parameters to sea bream (Sparus aurata) larvae

Parasites of wild sea bass Dicentrarchus labrax from Norway

Influence of Maternal and Larval Immunisation against <i>Lactococcus garviae</i> Infection in Rainbow Trout <i>Oncorhynchus mykiss</i> (Walaum) Lysozyme Activity and IgM Level

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First Report of Fish Pasteurellosis from Malta

Cited by 34 publications

References 0 publications

Maternal transfer of humoral specific and non-specific immune parameters to sea bream (Sparus aurata) larvae

Maternal transfer of humoral specific and non-specific immune parameters to sea bream (Sparus aurata) larvae

Parasites of wild sea bass Dicentrarchus labrax from Norway

Influence of Maternal and Larval Immunisation against &lt;i&gt;Lactococcus garviae&lt;/i&gt; Infection in Rainbow Trout &lt;i&gt;Oncorhynchus mykiss&lt;/i&gt; (Walaum) Lysozyme Activity and IgM Level

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Influence of Maternal and Larval Immunisation against <i>Lactococcus garviae</i> Infection in Rainbow Trout <i>Oncorhynchus mykiss</i> (Walaum) Lysozyme Activity and IgM Level