First report of extensively-drug-resistant Proteus mirabilis isolate carrying plasmid-mediated blaNDM-1 in a Tunisian intensive care unit

Kanzari, Lamia; Ferjani, Sana; Saïdani, Mabrouka; Hamzaoui, Zeineb; Jendoubi, Ali; Harbaoui, Sarra; Ferjani, Asma; Rehaiem, Amel; Boubaker, Ilhem Boutiba Ben; Slim, Aminé

doi:10.1016/j.ijantimicag.2018.06.009

Cited by 19 publications

(17 citation statements)

References 19 publications

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“…In proximity, the second cassette carried -aph(3 )-VI -ISAba125 -bla NDM-1 -∆qacE -sul1 -array flanked by the ISAba14 next to the aph(3 )-VI gene. The bla NDM-1 integration in this cassette is driven by ISAba125, as reported elsewhere [3]. The last cassette, harbouring tetB gene and its regulators (tetC and tetR), was flanked by an ISVsa5 (Figure 2).…”

Section: Resultsmentioning

confidence: 89%

“…The Gram-negative rod Proteus mirabilis is a leading cause of urinary tract infections (UTIs), particularly in patients suffering from complicated or catheter associated UTIs [1]. Because of the pathogen's intrinsic resistance to polymyxin, nitrofurantoin, and tigecycline [2], the additional acquisition of antimicrobial resistance genes represents an epidemiological and therapeutic threat [3]. Multi-drug resistance (MDR) profiles are constantly increasing worldwide in P. mirabilis, mainly due to the production of extended-spectrum β-lactamases (ESBLs), AmpC β-lactamases, and/or carbapenemases [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

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Complete Genome and Plasmids Sequences of a Clinical Proteus mirabilis Isolate Producing Plasmid Mediated NDM-1 From Italy

et al. 2020

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Background: The spread of carbapenemase genes, such as blaNDM-1, in Proteus mirabilis poses a public health threat. The aim of the study was to characterize the genome and plasmids sequences of an NDM-1-positive strain (IBCRE14), which was isolated in 2019 from a catheterized patient hospitalized in Italy. Methods: Whole genome sequencing (WGS) of IBCRE14 was performed on extracted genomic DNA using Sequel I platform. Genome assembly was performed using “Microbial Assembly”. Genomic analysis was conducted by uploading the contigs to ResFinder and PlasmidFinder databases from the Center for Genomic Epidemiology. Results: IBCRE14 had a genome size of 4,018,329 bp and harboured genes coding for resistance to aminoglycosides (aadA1), phenicol (cat), tetracycline (tetJ), and trimethoprim (dfrA1). A large plasmid (pIB_NDM_1) harboured antibiotic resistance genes against sulphonamide (sul1), trimethoprim (dfrA14), tetracycline (tetB), rifampicin (arr-2), aminoglycosides (aadA1, aph3-VI), and beta-lactams (blaOXA-10, blaNDM-1). Furthermore, a small plasmid (pIB_COL3M) harboured a qnrD1 gene coding for quinolone resistance. Conclusion: The ability to conjugate and the presence of a composite antibiotic resistance island suggests that pIB_NDM_1 could both acquire more resistance genes and easily disseminate. To our knowledge, this is the first report on an untypable plasmid harbouring blaNDM-1 in P. mirabilis, in Italy.

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Section: Resultsmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Complete Genome and Plasmids Sequences of a Clinical Proteus mirabilis Isolate Producing Plasmid Mediated NDM-1 From Italy

et al. 2020

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“…However, recent studies reported the detection of NDM-1 in Acinetobacter baumannii and Proteus mirabilis. 25,26 Concerning class D carbapenemases, we have noted the circulation of four major subfamilies in Tunisia, namely; OXA-48-like, OXA-23-like, OXA-51-like, and OXA-58-like (Table 1). Besides, three OXA-48-like variants (OXA-48, OXA-204, and OXA-232) were found in Enterobacteriaceae, most commonly Klebsiella pneumoniae.…”

Section: Carbapenemase Producing Gram-negative Bacteria From Hospitalsmentioning

confidence: 99%

“…48 Few antibiotics were destined for the treatment of these severe infections; polymyxin B (colimycine), polymyxin E (colistin), tigecycline, fosfomycin and some selected aminoglycosides that could be used in combination with carbapenems. 49 The clinical treatment of some carbapenemase producers seems to be extremely difficult, quoting the example of P. mirabilis which is intrinsically resistant to colistin and tigecycline; 26,50 emergence of such isolates constitutes a real threat.…”

Section: Treatmentsmentioning

confidence: 99%

<p>Carbapenemase Producing Gram-Negative Bacteria in Tunisia: History of Thirteen Years of Challenge</p>

Dziri

Salabi

et al. 2020

IDR

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The wide spread of multidrug-resistant bacteria, particularly carbapenem-resistant Gram-negative bacteria (CR-GNB), constitutes a major public health threat worldwide, owing to the limited therapeutic options. This review will describe and uncover the Tunisian experience in the challenge against carbapenem resistance. Indeed, we illuminate on the dissemination of CR-GNB in different hospitals, animals, and other natural environments in this country. We resumed the different carbapenemase variants detected from various bacterial species and mapped their regional distribution, basing on Tunisian published data during a period extended from 2006, the date of its first description in Tunisia, to February 2019. We also resumed the different mobile genetic elements implicated in their dissemination. This review shows that the majority of the research reports focused in the north and the coastal cities in spite of the fact that KPC and IMP carbapenemases were uncommonly detected in our country. However, VIM, NDM-1, and OXA-48 enzymes were usually reported with the predominance of OXA-48 among Enterobacteriaceae. Furthermore, OXA-23, OXA-51, and OXA-58 carbapenemases constituted the main mechanism conferring carbapenem resistance among Acinetobacter baumannii in Tunisia. Collaborative efforts and raising awareness of the threat of antibiotic resistance are required in order to minimize the spread of multidrug-resistant bacteria.

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“…In this context, Hamzaoui et al describe the role of association of OmpK35 and OmpK36 alteration and bla ESBL and/or bla AmpC in conferring carbapenem resistance among non-carbapenemase-producing Klebsiella pneumoniae [5] . The most recent metallo-β-lactamase, NDM-1, is particularly described in this Special Issue by Kanzari et al who describe the first report of an extensively drug-resistant Proteus mirabilis isolate carrying plasmid-mediated bla NDM-1 [6] and by Maamar et al who describe the incidence of NDM-1-and OXA-23 among clinical isolates of Acinetobacter baumannii [7] . In addition, Jaidane et al present a metagenomic analysis of the whole genome of NDM-1-producing ST85 A. baumannii isolates from Tunisia.…”

Section: Recent Advances In Antimicrobial and Bacterial Resistancementioning

confidence: 99%

Recent advances in antimicrobial and bacterial resistance

Chouchani¹,

Rolain

Ghrairi

2018

International Journal of Antimicrobial Agents

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HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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First report of extensively-drug-resistant Proteus mirabilis isolate carrying plasmid-mediated blaNDM-1 in a Tunisian intensive care unit

Cited by 19 publications

References 19 publications

Complete Genome and Plasmids Sequences of a Clinical Proteus mirabilis Isolate Producing Plasmid Mediated NDM-1 From Italy

Complete Genome and Plasmids Sequences of a Clinical Proteus mirabilis Isolate Producing Plasmid Mediated NDM-1 From Italy

<p>Carbapenemase Producing Gram-Negative Bacteria in Tunisia: History of Thirteen Years of Challenge</p>

Recent advances in antimicrobial and bacterial resistance

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