First report of Citrus psorosis virus in Japan

Ito, Takao; Furuta, Takane; Namba, Nobuyuki

doi:10.1007/s10327-011-0312-3

Cited by 5 publications

(4 citation statements)

References 7 publications

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“…Psorosis was a serious disease in many countries for several decades (Roistacher 1993). Although eradicated from Europe, it is still important in Argentina and Uruguay, due to its natural spread by an unidentified vector (Beñatena and Portillo 1984), in Texas, USA (Gottwald et al 2005), and more recently, the disease has been reported in countries where psorosis has never been detected before such as Cuba, Egypt, Turkey and Japan (Velazquez et al 2005;Ghazal et al 2008;Kayim 2010;Ito et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Improved Detection of Citrus psorosis virus and Coat Protein‐Derived Transgenes in Citrus Plants: Comparison Between RT‐qPCR and TAS‐ELISA

Francesco

Costa

Plata

et al. 2015

Journal of Phytopathology

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Citrus is one of the most economically important fruit crops in the world. Citrus psorosis is a serious disease affecting mainly oranges and mandarins in Argentina and Uruguay. The causal agent is Citrus psorosis virus (CPsV), an ophiovirus with a tripartite ssRNA genome of negative polarity. The coat protein (CP), the most abundant viral protein in infected plants, has been used to detect CPsV by TAS‐ELISA, but only biological indexing, requiring 1 year, is the current and validated technique for diagnosis of citrus psorosis. In this study, a SYBR Green RT‐qPCR protocol was developed, with primers designed to the most conserved region of the cp gene. We tested their specificity and sensitivity in comparison with TAS‐ELISA. This RT‐qPCR was applied successfully to field samples from Argentina, to a variety of isolates from different countries maintained in the greenhouse, to young seedlings and old trees from a psorosis natural transmission plot, and to transgenic citrus expressing the cp gene of CPsV or a fragment thereof. This method allowed accurate quantification of viral titer and cp gene expression in transgenic plants, which could not be detected previously. The sensitivity and reliability of quantitative CPsV detection were improved with greater speed using commercial reagents, and the sensitivity was three orders of magnitude higher than that of TAS‐ELISA. All these data encourage its validation.

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Section: Introductionmentioning

confidence: 99%

Improved Detection of Citrus psorosis virus and Coat Protein‐Derived Transgenes in Citrus Plants: Comparison Between RT‐qPCR and TAS‐ELISA

Francesco

Costa

Plata

et al. 2015

Journal of Phytopathology

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“…× C. sinensis (L.) Osbeck] × C. reticulata Blanco, in the experimental field of the National Agricultural Research Organization. Neither tree exhibited the bark-scaling symptom typical of CPsV infections (Ito et al 2011). In countries surrounding Japan, CPsV has been detected in China (Zhou 2018).…”

Section: Other Virusesmentioning

confidence: 99%

Citrus Viroids and Minor Citrus Viruses in Japan

Iwanami

2023

JARQ

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Citrus species grown in Japan are affected by several viruses and viroids, including satsuma dwarf virus, citrus vein enation virus, citrus exocortis viroid, and other citrus viroids. Recently, a comprehensive review was made on the occurrence, history, and research of the following three major citrus viruses in Japan: satsuma dwarf virus, citrus tatter leaf virus (apple stem grooving virus), and citrus tristeza virus (Iwanami 2022). As a sequel, this study reviews the occurrence, history, and research development of citrus viroids as well as minor citrus viruses in and around Japan.

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“…Phylum Negarnaviricota, kingdom Orthornavirae Order Serpentovirales The order Serpentovirales consists of one family, Aspiviridae (former Ophioviridae), one genus, Ophiovirus, and seven species (Table 1 and Supplementary Table S1). Ophioviruses infect only plants, and citrus psorosis virus (CPsV) (Ito et al 2011) and Mirafiori lettuce big-vein virus (MiL-BVV) are present worldwide and cause serious problems for citrus and lettuce production, respectively. MiLBVV, lettuce ring necrosis virus, tulip mild mottle mosaic virus (TMMMV), and freesia sneak virus (FreMV) are transmitted by soil-inhabiting fungi of the genus Olpidium.…”

Section: Realm Riboviria Phylum Duplornaviricota Kingdom Orthornavira...mentioning

confidence: 99%

“…The causal agent of the mild mottle mosaic diseases of tulip (TMMMV) was thought to be an ophiovirus (Morikawa et al 1995), and later, its partial genomic sequence was confirmed via RT-PCR (Vaira et al 2003). Along with TMMMV, four other ophioviruses (MiLBVV, CPsV, FreMV, and BlMaV) have been reported in Japan (Isogai et al 2016;Ito et al 2011;Natsuaki et al 2002).…”

Section: Realm Riboviria Phylum Duplornaviricota Kingdom Orthornavira...mentioning

confidence: 99%

Plant viruses and viroids in Japan

et al. 2022

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An increasing number of plant viruses and viroids have been reported from all over the world due largely to metavirogenomics approaches with technological innovation. Herein, the official changes of virus taxonomy, including the establishment of megataxonomy and amendments of the codes of virus classification and nomenclature, recently made by the International Committee on Taxonomy of Viruses were summarized. The continued efforts of the plant virology community of Japan to index all plant viruses and viroids occurring in Japan, which represent 407 viruses, including 303 virus species and 104 unclassified viruses, and 25 viroids, including 20 species and 5 unclassified viroids, as of October 2021, were also introduced. These viruses and viroids are collectively classified into 81 genera within 26 families of 3 kingdoms (Shotokuvirae, Orthornavirae, Pararnavirae) across 2 realms (Monodnaviria and Riboviria). This review also overviewed how Japan’s plant virus/viroid studies have contributed to advance virus/viroid taxonomy.

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First report of Citrus psorosis virus in Japan

Cited by 5 publications

References 7 publications

Improved Detection of Citrus psorosis virus and Coat Protein‐Derived Transgenes in Citrus Plants: Comparison Between RT‐qPCR and TAS‐ELISA

Improved Detection of Citrus psorosis virus and Coat Protein‐Derived Transgenes in Citrus Plants: Comparison Between RT‐qPCR and TAS‐ELISA

Citrus Viroids and Minor Citrus Viruses in Japan

Plant viruses and viroids in Japan

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