First report of Chilli veinal mottle virus in Naga chilli (Capsicum chinense) in Meghalaya, India

Banerjee, Amrita; Dutta, Ram; Roy, Somnath; Ngachan, S. V.

doi:10.1007/s13337-013-0167-7

Cited by 16 publications

(10 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chilli is reported to be affected by more than 30 economically important viruses by causing 15–50% of yield losses in chilli cultivation 2 , 3 . It includes Cucumber mosaic virus 4 , Chilli leaf curl virus 5 , Tomato spotted wilt virus 6 , Groundnut bud necrosis virus 7 , Capsicum chlorosis virus 8 , Tobacco mosaic virus 9 , Potato virus Y 6 , Tomato leaf curl New Delhi virus 10 and Chilli veinal mottle virus 11 . Among them the cucumo and tospoviruses are the most predominant viruses reported to infect almost all the vegetables crops and pose serious threat to chilli production 12 .…”

Section: Introductionmentioning

confidence: 99%

Co-existence of chlorosis inducing strain of Cucumber mosaic virus with tospoviruses on hot pepper (Capsicum annuum) in India

Vinodhini

Rajendran

Abirami

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Cucumo- and tospoviruses are the most destructive viruses infecting hot pepper (chilli). A diagnostic survey was conducted to assess the prevalence of cucumo and tospoviruses in chilli growing tracts of Tamil Nadu. Infected plants showing mosaic with chlorotic and necrotic rings, veinal necrosis, mosaic mottling, leaf filiformity and malformation were collected. Molecular indexing carried out through reverse transcription polymerase chain reaction (RT-PCR) with coat protein gene specific primer of Cucumber mosaic virus (CMV) and tospovirus degenerate primer corresponding to the L segment (RdRp). Ostensibly, amplifications were observed for both CMV and tospoviruses as sole as well for mixed infections. The sequence analysis indicated that the Capsicum chlorosis virus (CaCV) and Groundnut bud necrosis virus (GBNV) to be involved with CMV in causing combined infections. The co-infection of CMV with CaCV was detected in 10.41% of the symptomatic plant samples and combined infection of CMV with GBNV was recorded in around 6.25% of the symptomatic plants surveyed. The amino acid substitution of Ser129 over conserved Pro129 in coat protein of CMV implies that CMV strain involved in mixed infection as chlorosis inducing strain. Further, the electron microscopy of symptomatic plant samples explicated the presence of isometric particles of CMV and quasi spherical particles of tospoviruses. This is the first molecular evidence for the natural co-existence of chlorosis inducing CMV strain with CaCV and GBNV on hot pepper in India.

show abstract

Section: Introductionmentioning

confidence: 99%

Co-existence of chlorosis inducing strain of Cucumber mosaic virus with tospoviruses on hot pepper (Capsicum annuum) in India

Vinodhini

Rajendran

Abirami

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Biotic constraints particularly symptoms akin to viral infection has long been recorded as a major hurdle to the successful production of these chillies in the region. High incidence of viral infection as recorded through visual observations of symptoms and recent report of ChiVMV infection on Naga chilli from Meghalaya indicated the possibility of its association with chilli decline in the NE region (Banerjee et al 2014). NE region being contagious to the Eastern Asian region provided a strong base for the possible prevalence of pathogenically and genetically diverse ChiVMV isolates.…”

Section: Discussionmentioning

confidence: 96%

“…Based on the sequencing of a genomic fragment, association of chilli leaf curl virus (CLCuV) with Capsicum chinense was also reported (Adluri et al 2016). Recently, first authentic molecular evidence for the infection of Chilli veinal mottle virus (ChiVMV) in Capsicum chinense from low to moderate temperature geographical areas of NE India was provided (Banerjee et al 2014). ChiVMV was suspected to be responsible in bringing havoc in the chilli plantations in Manipur also, where similar low to moderate temperature conditions prevails.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Chilli veinal mottle virus infecting different chilli landraces in North East India indicates the possibility of transboundary movement of virus

et al. 2018

View full text Add to dashboard Cite

Diverse chilli genetic resources of North East India occupy a unique niche in the Nation's chilli gene pool. Widely cultivated chilli landraces (King chilli: , bird eye chilli: and ) of North East India in general and Manipur in particular suffer from decline complex due to high incidence of viral diseases. With this background and the known prominent distribution of (ChiVMV) in Asian region, we studied its prevalence and association with diverse symptoms of chilli landraces. Molecular indexing of samples from 40 chilli plantation groves of Manipur using reverse transcription (RT)-PCR targeting the coat protein (CP) region of ChiVMV genome revealed a high incidence and wide prevalence. Out of a total of 127 chilli samples collected from different groves of Manipur, 81 chilli samples (63.78%) were positive for ChiVMV. Interestingly, ChiVMV infection rate was comparatively higher in the hilly groves (69.23% samples positive) compared to the valley groves (60% samples positive). Present study through the extensive surveys and molecular indexing work, conclusively reported the association of ChiVMV with diverse symptoms like cupping of leaf lamina with mottling, vein banding and puckering in different chilli landraces. Further, five representative ChiVMV isolates sampled from different groves of Manipur upon mechanical inoculation showed significant variation in symptom expression, indicating wide pathogenic diversity among them. Partial coat protein (CP) sequence analysis of five ChiVMV isolates from Manipur although indicated genetic homogeneity among them, but distinctiveness from ChiVMV isolates reported from the other parts of India. Phylogenetic clustering of ChiVMV isolates from Manipur near Chinese isolates rather than other Indian isolates suggested the possibility of transboundary movement.

show abstract

“…Reports are available on the survey of viral disease incidence in Bhutjolokia from Assam based on symptomatology, transmission, host range and serological assays (Baruah et al, 2016;Talukdar et al, 2015Talukdar et al, , 2017. Molecular method have also been used for the detection and confirmation of viruses including ChiVMV (Banerjee et al 2014) in Meghalaya and ChiLCV (Baruah et al 2016) in Assam. Talukdar et al (2015Talukdar et al ( , 2017 reported the viral disease incidence on Bhutjolokia in Assam based on DASELISA to be 52%, 52% and 42% for Cucumovirus, Potyvirus and Tospovirus respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Different researches have shown over time that the crop is susceptible to different viruses which include Cucumber mosaic virus (CMV), Potato virus Y (PVY) (Talukdar et al, 2015;Baruah et al, 2016), Tomato Spotted Wilt Virus (TSWV) (Talukdar et al, 2015), Chilli leaf curl virus (ChLCV) ( Talukdar et al, 2015;Baruah et al, 2016) and Groundnut Bud Necrosis Virus (GBNV) (Baruah et al, 2016). Bhut jolokia has also been found to be susceptible to Chilliveinal mottle virus as reported from Meghalaya (Sanabam et al, 2018;Banerjee et al, 2014). The plants are at times infected by a single virus or collectively by different viruses thereby forming a viral complex (Baruah et al, 2016) which reduces the production and productivity to greater extent.…”

Section: Introductionmentioning

confidence: 96%

Molecular Characterization of Chilli Veinal Mottle Virus Infecting King Chilli (Capsicum Chinense Jacq.) of North East Indian State Assam

BORAH¹,

ROUTHU²,

DUTTA³

et al. 2022

AJMBES

View full text Add to dashboard Cite

King chilli crop of Assam, India infected with Chilliveinal mottle virus (ChiVMV) belongs togenus potyvirus of the family potyviridae suffered from necrosis of leaves, leaf puckering, filiform leavesand eventually plant death. King chilli crop particularly suffers from decline complex due to high incidenceof viral diseases. Prominent distribution of Chilliveinal mottle virus (ChiVMV) in King chilli growingregion of Jorhat and Golaghat districts of Assam state were considered to study the prevalence andassociation with symptoms associated. Molecular indexing of samples from 30 chilli plantation orchards ofJorhat and Golaghat districts using reverse transcription (RT)-PCR targeting the coat protein (CP) region ofChiVMV genome revealed a high incidence and wide prevalence of the virus. Out of a total of 96 chillisamples collected from different orchards of Jorhat and Golaghat districts, 41 chilli samples (42.70%) werepositive for ChiVMV. From Golaghat out of 34 samples 14 were found positive to ChiVMoV (41.17%),whereas in Jorhat out of 62 samples 27 were found positive to the virus (43.54%). Present study through theextensive surveys and molecular indexing work, conclusively reported the association of ChiVMV withdiverse symptoms likemosaic/mottling, vein banding, leaf puckering /rugose/leaf distortion, narrow leaflamina, shoe-string of leaves/leaf curling and stunted growth in King chilli. ChiVMV isolate from Jorhat,Assam were characterized based on sequencing of coat protein (CP) gene and phylogenetic analysis.Sequence analysis of CP gene showed that ChiVMV from Jorhat shared 100% identity with China isolates.This was reported as the first record of ChiVMVincidence in King chillicrop of Assam.

show abstract

First report of Chilli veinal mottle virus in Naga chilli (Capsicum chinense) in Meghalaya, India

Cited by 16 publications

References 6 publications

Co-existence of chlorosis inducing strain of Cucumber mosaic virus with tospoviruses on hot pepper (Capsicum annuum) in India

Co-existence of chlorosis inducing strain of Cucumber mosaic virus with tospoviruses on hot pepper (Capsicum annuum) in India

Genetic diversity of Chilli veinal mottle virus infecting different chilli landraces in North East India indicates the possibility of transboundary movement of virus

Molecular Characterization of Chilli Veinal Mottle Virus Infecting King Chilli (Capsicum Chinense Jacq.) of North East Indian State Assam

Contact Info

Product

Resources

About