First Report of Boscalid Resistant <i>Botrytis cinerea</i> Isolates Carrying the Mutations H272R, H272Y, P225L, and P225H from Table Grape in Chile

Esterio, M.; Araneda, M. J.; Román, Andrea; Pizarro, Lorena; Copier, Ch.; Auger, J.

doi:10.1094/pdis-12-14-1257-pdn

Cited by 13 publications

(7 citation statements)

References 3 publications

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“…Esterio et al (2015) reported the detection of the H272R/Y/L and P255L/H point mutations in table grapes at frequencies of 52.8%, 35.42%, 2.1%, 6.25% and 4.16%, respectively. The introduction of new SDHI fungicides in Chile, such as fluopyram, may modify the structure of local B. cinerea populations.…”

Section: Discussionmentioning

confidence: 99%

“…During the last decade, pyrimethanil, azoxystrobin, boscalid, fenhexamid and fludioxonil fungicides have been used extensively to control gray mold disease in table grapes in Chile, and fungicide resistance of B. cinerea has been reported (Esterio et al, 2007(Esterio et al, , 2012(Esterio et al, , 2015Latorre et al, 2002;Piqueras et al, 2014). We hypothesized that the frequency of multiple-resistance in B. cinerea populations would increase; thus, it would be crucial to monitor such frequency in table grape orchards to evaluate the risk of infection for specific areas and growing conditions and elaborate appropriate anti-resistance strategies.…”

Section: Introductionmentioning

confidence: 99%

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Frequency of fungicide-resistant Botrytis cinerea populations isolated from ‘Thompson Seedless’ table grapes in the Central Valley of Chile

Grez¹,

Copier²,

Román³

et al. 2017

Ciencia e investigación agraria

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were sensitive to all fungicides tested; from north to south, the frequency of sensitive isolates in the regions of Valparaiso, Metropolitana and O'Higgins ranged from 48.15% to 21.1% and 5.88%, respectively. Four hundred and twenty isolates (79%) showed resistance to single or multiple fungicides, 134 (25.4%) were simultaneously resistant to azoxystrobin and pyrimethanil. No fludioxonil-resistant isolates were found, indicating that fludioxonil has great potential for gray mold control in table grapes in Chile. From sixty randomly selected B. cinerea isolates, only the azoxystrobin-resistant isolates carried the G143A point mutation; according to the cytochrome b (cyt b) gene structure, the third intron Bcbi-143/144 was only detected in the azoxystrobin-sensitive isolates. The H272R and H272Y point mutations in the succinate dehydrogenase subunit B (sdhB) gene were associated only with the boscalid-resistant isolates. The F412S and F412V point mutations were found in the sequenced erg27 gene of randomly selected fenhexamid-resistant isolates. These results contribute to the knowledge of B. cinerea fungicide resistance for table grape vine crops in Central Chile, particularly for the development of multiple-resistance and the associated resistance mechanisms of azoxystrobin, boscalid and fenhexamid-resistant isolate populations. Antiresistance strategies are discussed in a general manner.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Frequency of fungicide-resistant Botrytis cinerea populations isolated from ‘Thompson Seedless’ table grapes in the Central Valley of Chile

Grez¹,

Copier²,

Román³

et al. 2017

Ciencia e investigación agraria

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“…In this context, fungicides are the most common control method for these pathogens [10]; indeed, table grape and wine sectors represent 50% of the total market value for fungicides [10]. However, fungicide resistance is a growing problem [11][12][13][14][15]. Botrytis management's overall expenses, including cultural measures, botrycides, broad-spectrum fungicides, and biocontrols, easily reach €1 billion/year in all countries; product and quality loss in the market chain, however, is probably much higher [10].…”

Section: Introductionmentioning

confidence: 99%

Volatile Organic Compounds (VOCs) Produced by Gluconobacter cerinus and Hanseniaspora osmophila Displaying Control Effect against Table Grape-Rot Pathogens

et al. 2021

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Table grapes (Vitis vinifera) are affected by botrytis bunch rot and summer bunch rot, the latter a complex disease caused by Botrytis cinerea, Aspergillus spp., Penicillium expansum and Rhizopus stolonifer. To search for biocontrol alternatives, a new bioproduct composed of Gluconobacter cerinus and Hanseniaspora osmophila, a consortium called PUCV-VBL, was developed for the control of fungal rots in table grapes. Since this consortium presents new biocontrol species, the effect of their VOCs (volatile organic compounds) was evaluated under in vitro and in vivo conditions. The VOCs produced by the PUCV-VBL consortium showed the highest mycelial inhibition against Botrytis cinerea (86%). Furthermore, H. osmophila was able to inhibit sporulation of A. tubingensis and P. expansum. VOCs’ effect in vivo was evaluated using berries from Red Globe, Thompson Seedless and Crimson Seedless grapes cultivars, demonstrating a mycelial inhibition by VOCs greater than 70% for all evaluated fungal species. The VOC identification of the PUCV-VBL consortium was analyzed by solid-phase microextraction coupled to gas chromatography-mass spectrometry (SPME-GCMS). A total 26 compounds were identified, including 1-butanol 3-methyl, propanoic acid ethyl ester, ethyl acetate, phenylethyl alcohol, isobutyl acetate and hexanoic acid ethyl ester. Our results show that VOCs are an important mode of action of the PUCV-VBL biological consortium.

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“…Other mutations such as H272Y or N230I were found in lower frequencies, while P225H was detected in a very low frequency in the population originating from stone fruit rootstock seedlings. P225H mutation is a rather rare mutation that, till now, has been reported only in B. cinerea originating from stone fruit rootstock seedlings in Greece (Konstantinou et al, 2014) and from table grapes in Chile (Esterio et al, 2015). In most of the monitoring studies published previously these mutations are rare or in lower frequencies compared to the frequency of H272R mutation.…”

Section: Discussionmentioning

confidence: 99%

“…Sequence analysis of the four sdh subunits in SDHI-resistant strains revealed that resistance was associated with nine target site mutations located in the sdh B subunit and one mutation in the sdh D subunit. In the sdh B subunit mutations associated with resistance to SDHIs have been detected at codons 225, 230, and 272 (Leroux et al, 2010; Veloukas et al, 2011; De Miccolis Angelini et al, 2014a; Esterio et al, 2015). At codon 225 the proline can be substituted by phenylalanine (P225F), leucine (P225L), threonine (P225T), or histidine (P225H).…”

Section: Introductionmentioning

confidence: 99%

Detection of sdhB Gene Mutations in SDHI-Resistant Isolates of Botrytis cinerea Using High Resolution Melting (HRM) Analysis

2016

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Botrytis cinerea, is a high risk pathogen for fungicide resistance development. Pathogen’ resistance to SDHIs is associated with several mutations in sdh gene. The diversity of mutations and their differential effect on cross-resistance patterns among SDHIs and the fitness of resistant strains necessitate the availability of a tool for their rapid identification. This study was initiated to develop and validate a high-resolution melting (HRM) analysis for the identification of P225H/F/L//T, N230I, and H272L/R/Y mutations. Based on the sequence of sdhB subunit of resistant and sensitive isolates, a universal primer pair was designed. The specificity of the HRM analysis primers was verified to ensure against the cross-reaction with other fungal species and its sensitivity was evaluated using concentrations of known amounts of mutant’s DNA. The melting curve analysis generated nine distinct curve profiles, enabling the discrimination of all the four mutations located at codon 225, the N230I mutation, the three mutations located in codon 272, and the non-mutated isolates (isolates of wild-type sensitivity). Similar results were obtained when DNA was extracted directly from artificially inoculated strawberry fruit. The method was validated by monitoring the presence of sdhB mutations in samples of naturally infected strawberry fruits and stone fruit rootstock seedling plants showing damping-off symptoms. HRM analysis data were compared with a standard PIRA–PCR technique and an absolute agreement was observed suggesting that in both populations the H272R mutation was the predominant one, while H272Y, N230I, and P225H were detected in lower frequencies. The results of the study suggest that HRM analysis can be a useful tool for sensate, accurate, and rapid identification of several sdhB mutations in B. cinerea and it is expected to contribute in routine fungicide resistance monitoring or assessments of the effectiveness of anti-resistance strategies implemented in crops heavily treated with botryticides.

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First Report of Boscalid Resistant Botrytis cinerea Isolates Carrying the Mutations H272R, H272Y, P225L, and P225H from Table Grape in Chile

Abstract: Artículo de publicación ISISin acceso a texto complet

Cited by 13 publications

References 3 publications

Frequency of fungicide-resistant Botrytis cinerea populations isolated from ‘Thompson Seedless’ table grapes in the Central Valley of Chile

Frequency of fungicide-resistant Botrytis cinerea populations isolated from ‘Thompson Seedless’ table grapes in the Central Valley of Chile

Volatile Organic Compounds (VOCs) Produced by Gluconobacter cerinus and Hanseniaspora osmophila Displaying Control Effect against Table Grape-Rot Pathogens

Detection of sdhB Gene Mutations in SDHI-Resistant Isolates of Botrytis cinerea Using High Resolution Melting (HRM) Analysis

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