First records of Pythium aquatile and P. macrosporum isolated from soils in Japan

Uzuhashi, Shihomi; Tojo, Motoaki; Kobayashi, Sakura; Tokura, Katsuyuki; Kakishima, Makoto

doi:10.1007/s10267-008-0416-6

Cited by 16 publications

(12 citation statements)

References 13 publications

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“…Morphological identification of these oomycetes was based on van der Plaats-Niterink (1981) and Erwin and Ribeiro (1996). To confirm morphological identification further, we determined the rDNA ITS sequences of the isolates by a method described by Uzuhashi et al (2008). The species identity of isolates was estimated by conducting a BLAST analysis for each sequence.…”

Section: Identification Of Pythium and Phytophthora Isolatesmentioning

confidence: 99%

Association of Pythium and Phytophthora with Pre-emergence Seedling Damping-off of Soybean Grown in a FieldConverted from a Paddy Field in Japan

Kato

Minamida

Tojo

et al. 2013

Plant Production Science

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In Japan, soybean is usually cultivated in fields that have been converted from rice paddies, and poor seedling establishment due to pre-emergence seedling damping-off is often observed during the rainy season. In this study, the factors that cause the damping-off in flooded soil were investigated under high soil moisture conditions in a greenhouse and in agricultural fields. In sterilized soil sampled from a soybean field, seedlings emerged well under 48-hr flooded conditions. In unsterilized soil, soybean seeds treated with the fungicide, mancozeb +metalaxyl exhibited much higher emergence rates even under 48-hr flooded conditions than the seeds treated with oxytetracycline +streptomycin, benomyl, or flutolanil. Pythium, Phytophthora, Mucorales, Trichoderma, Geotrichum-like microorganisms, and some fungi producing conidia in a false head, were isolated from decayed seedlings. Of the isolated microorganisms, oomycete microorganisms, Pythium helicoides, other Pythium sp., and Phytophthora sp. were pathogenic to soybeans under flooded conditions. As the length of the flooding period increased, pre-emergence seedling rot also increased. However, the pathogenic oomycetes rarely caused pre-emergence seedling rot under conditions without flooding. Furthermore, under flooded conditions, the damage caused by these pathogens was reduced by treating the seeds with mancozeb+metalaxyl. These results indicate that oomycete microorganisms are involved in pre-emergence seedling damping-off under flooded soil conditions.

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Section: Identification Of Pythium and Phytophthora Isolatesmentioning

confidence: 99%

Association of Pythium and Phytophthora with Pre-emergence Seedling Damping-off of Soybean Grown in a FieldConverted from a Paddy Field in Japan

Kato

Minamida

Tojo

et al. 2013

Plant Production Science

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“…Pythium macrosporum has been isolated in several countries, including Canada, Germany, Japan, the Netherlands and the United States (Allain-Boule et al 2004;Uzuhashi et al 2008;Van der Plaäts-Niterink 1981;Van Os et al 1999;Westover and Bever 2001), and is known to cause root rots in flower bulbs (Westover and Bever 2001), grasses (Van Os et al 1999), and carrot (Allain-Boule et al 2004). This species was detected in diseased soybean roots using a Pythium DNA array hybridization method during an extensive survey for root rot pathogens in commercial fields of soybean in eastern Ontario and Quebec (Barasubiye et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity of Pythium species causing seed rot and damping-off in soybean under controlled conditions

Wei

Xue

Cober

et al. 2012

phyto

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Montréal. Il a pour mission la promotion et la valorisation de la recherche. Érudit offre des services d'édition numérique de documents scientifiques depuis 1998.Pour communiquer avec les responsables d'Érudit : info@erudit.org Article "Pathogenicity of Pythium species causing seed rot and damping-off in soybean under controlled conditions" Lai Wei, Allen G. Xue, Elroy R. Cober, Carolyn Babcock, Jinxiu Zhang, Shuzhen Zhang, Wenbin Li, Junjiang Wu et Lijun Liu Phytoprotection, vol. 91, 2010Phytoprotection, vol. 91, -2011 Pour citer cet article, utiliser l'information suivante : Pythium species cause seed rot (SR) and damping-off (DO) in soybean worldwide. In a previous study, a number of Pythium species were isolated from infected soybean plants across Ontario and Quebec, but their comparative pathogenicities to soybean were not examined. In the present research, 24 isolates from eight Pythium spp. were evaluated for their pathogenicity in causing soybean SR and DO in a greenhouse environment. The effect of temperature on the ability of these isolates to cause SR was also studied. There were significant differences among the eight Pythium spp. for both SR and DO. When tested at 25°C, Pythium ultimum was the most pathogenic species, causing 97.0% SR and 46.4% DO, on average, in the two soybean cultivars used. Pythium aphanidermatum was the second most pathogenic species, resulting in 88.5% SR and 41.8% DO. The two species resulted in significantly greater SR and DO than the other six species tested and were considered highly pathogenic. Of the two cultivars used in these trials, 'Beechwood' was significantly more susceptible than 'Nattawa' to both SR and DO. Temperature had a significant influence on SR caused by Pythium spp. At all four temperatures tested (4°C, 12°C, 20°C and 28°C), P. ultimum was highly pathogenic, while P. arrenomanes, P. coloratum and P. dissotocum were the least pathogenic. The interactions between temperature and Pythium spp. were more pronounced for P. aphanidermatum, which showed an increased percentage of SR with an increase in temperature, and for P. irregulare, P. macrosporum and P. sylvaticum, which showed a decreased percentage of SR with an increase in temperature.Keywords: damping-off, Glycine max, pathogenicity, Pythium spp., seed rot, soybean.[Pouvoir pathogène d'espèces de Pythium provoquant la pourriture de racine et la fonte des semis chez la fève de soja en conditions contrôlées] Les espèces de Pythium provoquent la pourriture de racine (PR) et la fonte des semis (FS) chez la fève de soja dans le monde entier. Dans une étude précédente, des espèces de Pythium ont été isolées à partir de plants de fève de soja infectés en Ontario et au Québec, mais leur pouvoir pathogène n'a pas été évalué. Dans la présente recherche, le pouvoir pathogène de 24 isolats de huit espèces de Pythium a été évalué relativement à leur capacité de provoquer la PR et la FS dans des serres; l'effet de la température sur leur capacité de provoquer la PR a également été étudié. Il y avait des différences...

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“…The nucleotide sequences of the ITS region including 5.8S rDNA of isolates UZ041 and UZ415 were determined as described previously (Uzuhashi et al 2008). Both sequences were 875 bp long, and they were identical each other.…”

Section: Sequence Analysismentioning

confidence: 99%

Characterization of Pythium nunn newly recorded in Japan and its antagonistic activity against P. ultimum var. ultimum

et al. 2010

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Two isolates of Pythium nunn, newly recorded in Japan, were obtained from soils in Nagano and Fukuoka prefectures, characterized, and tested for antagonistic activity against P. ultimum var. ultimum. The morphology of both isolates corresponded with those of the original description of P. nunn. The rDNA-ITS sequences of the two isolates were identical to each other and had a high similarity with the sequences of the type strain of P. nunn. The two P. nunn isolates were mycoparasitic toward P. ultimum var. ultimum and suppressed damping off of cucumber seedlings caused by the pathogen at an early stage of plant growth.

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First records of Pythium aquatile and P. macrosporum isolated from soils in Japan

Abstract: Pythium aquatile and P. macrosporum were isolated from the soil of a cultivated fi eld in Gunma Prefecture and a forest in Nagano Prefecture for the fi rst time in Japan. Their morphological characteristics are described, and their pathogenicity and taxonomy are discussed.

Cited by 16 publications

References 13 publications

Association of Pythium and Phytophthora with Pre-emergence Seedling Damping-off of Soybean Grown in a FieldConverted from a Paddy Field in Japan

Association of Pythium and Phytophthora with Pre-emergence Seedling Damping-off of Soybean Grown in a FieldConverted from a Paddy Field in Japan

Pathogenicity of Pythium species causing seed rot and damping-off in soybean under controlled conditions

Characterization of Pythium nunn newly recorded in Japan and its antagonistic activity against P. ultimum var. ultimum

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