Pathogenicity of Pythium species causing seed rot and damping-off in soybean under controlled conditions

Wei, Lai; Xue, Allen; Cober, Elroy R.; Babcock, C. E.; Zhang, Jinxiu; Zhang, Shuzhen; Li, Wenbin; Wu, Jianzhong; Liu, Lijun

doi:10.7202/1008539ar

Cited by 32 publications

(14 citation statements)

References 27 publications

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“…Pythium sylvaticum was the most prevalent species detected across the midwestern United States on diseased soybean seedlings ( Broders et al, 2007 ; Wei et al, 2010 ; Jiang et al, 2012 ; Rojas et al, 2017 ). We used this species to test for resistance in three of our four populations (POP1, POP3, and POP4).…”

Section: Discussionmentioning

confidence: 99%

Identification of Quantitative Disease Resistance Loci Toward Four Pythium Species in Soybean

et al. 2021

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In this study, four recombinant inbred line (RIL) soybean populations were screened for their response to infection by Pythium sylvaticum, Pythium irregulare, Pythium oopapillum, and Pythium torulosum. The parents, PI 424237A, PI 424237B, PI 408097, and PI 408029, had higher levels of resistance to these species in a preliminary screening and were crossed with “Williams,” a susceptible cultivar. A modified seed rot assay was used to evaluate RIL populations for their response to specific Pythium species selected for a particular population based on preliminary screenings. Over 2500 single-nucleotide polymorphism (SNP) markers were used to construct chromosomal maps to identify regions associated with resistance to Pythium species. Several minor and large effect quantitative disease resistance loci (QDRL) were identified including one large effect QDRL on chromosome 8 in the population of PI 408097 × Williams. It was identified by two different disease reaction traits in P. sylvaticum, P. irregulare, and P. torulosum. Another large effect QDRL was identified on chromosome 6 in the population of PI 408029 × Williams, and conferred resistance to P. sylvaticum and P. irregulare. These large effect QDRL will contribute toward the development of improved soybean cultivars with higher levels of resistance to these common soil-borne pathogens.

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Section: Discussionmentioning

confidence: 99%

Identification of Quantitative Disease Resistance Loci Toward Four Pythium Species in Soybean

et al. 2021

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“…Among the tested isolates, GZ-11 isolate was found to be the highest pathogenic, causing seed rot and seedling damping-off at rate 100%, and selected to be identified. Wei et al (2011) in study with eight Pythium spp reported that Pythium ultimum was the most pathogenic species resulting in 97% seed rot and 46.4% damping-off on soybean. Rossman et al (2017) observed variations in the virulence of the Pythium isolates in causing seed rot and pre-emergence damping-off of dry bean.…”

Section: Pathogenicity Of Pythium Sppmentioning

confidence: 98%

Morphological Characterization and Pathogenicity of pythium sp. Infecting Cucumber Seedlings in Greenhouses

Abo-Zaid

Bashir

Hafez

2020

Alexandria Science Exchange Journal

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Greenhouse-grown cucumber seedlings exhibiting typical symptoms of Pythium damping-off and root rot were collected. The causal agents were isolate on Pythium selective medium; nystatin, ampicillin, rifampicin, and miconazole (NARM) in potato dextrose agar (PDA). Four different isolates was isolated and their pathogenicity was tested using cotyledon and young seedlings assays. Results of these isolates showed variation in their ability to infect cucumber seedling. Isolate "GZ-11" was highly pathogenic, caused 100 % pre-and post-emergence damping-off. The isolate was identified to the species level based on morphological characters along with sequences of the internal transcribed spacer (ITS) of the ribosomal DNA and showed 99% similarity to Pythium spinosum (Synonym of Globisporangium spinosum). Aggressiveness of P. spinosum was investigated at two levels of inoculum concentrations (10 and 20 g/kg soil). Results reported that P. spinosum was highly aggressive and significantly reduced cucumber seed germination compared to the control.

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“…Because longer growing seasons directly correlate with increased soybean yields, early planting is the current preferred practice ( Rowntree et al, 2013 ). However, cold soils, coupled with an unseasonably wet planting season, increases the risk of oomycete-mediated root rot seedling loss ( Broders et al, 2007 ; Wei et al, 2011 ; Zitnick-Anderson and Nelson, 2015 ). Direct sampling of infected plants found that a wide range of pathogens were involved.…”

Section: Introductionmentioning

confidence: 99%

“…Some of these Pythium species such as P. irregulare, P. sylvaticum, P. torulosum , and P. macrosporum were cold adapted and most aggressive at soil temperatures below 20°C. Other species such as P. ultimum infect different hosts at temperatures ranging from 12 to 25°C ( Wei et al, 2011 ), while P. aphanidermatum cause soybean seedling rot only at temperatures of 20–25°C ( Rojas et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Aquatic Pseudomonads Inhibit Oomycete Plant Pathogens of Glycine max

Wagner¹,

Norris²,

Chatterjee³

et al. 2018

Front. Microbiol.

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Seedling root rot of soybeans caused by the host-specific pathogen Phytophthora sojae, and a large number of Pythium species, is an economically important disease across the Midwest United States that negatively impacts soybean yields. Research on biocontrol strategies for crop pathogens has focused on compounds produced by microbes from soil, however, recent studies suggest that aquatic bacteria express distinct compounds that efficiently inhibit a wide range of pathogens. Based on these observations, we hypothesized that freshwater strains of pseudomonads might be producing novel antagonistic compounds that inhibit the growth of oomycetes. To test this prediction, we utilized a collection of 330 Pseudomonas strains isolated from soil and freshwater habitats, and determined their activity against a panel of five oomycetes: Phytophthora sojae, Pythium heterothalicum, Pythium irregulare, Pythium sylvaticum, and Pythium ultimum, all of which are pathogenic on soybeans. Among the bacterial strains, 118 exhibited antagonistic activity against at least one oomycete species, and 16 strains were inhibitory to all pathogens. Antagonistic activity toward oomycetes was significantly more common for aquatic isolates than for soil isolates. One water-derived strain, 06C 126, was predicted to express a siderophore and exhibited diverse antagonistic profiles when tested on nutrient rich and iron depleted media suggesting that more than one compound was produced that effectively inhibited oomycetes. These results support the concept that aquatic strains are an efficient source of compounds that inhibit pathogens. We outline a strategy to identify other strains that express unique compounds that may be useful biocontrol agents.

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Pathogenicity of Pythium species causing seed rot and damping-off in soybean under controlled conditions

Cited by 32 publications

References 27 publications

Identification of Quantitative Disease Resistance Loci Toward Four Pythium Species in Soybean

Identification of Quantitative Disease Resistance Loci Toward Four Pythium Species in Soybean

Morphological Characterization and Pathogenicity of pythium sp. Infecting Cucumber Seedlings in Greenhouses

Aquatic Pseudomonads Inhibit Oomycete Plant Pathogens of Glycine max

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